Cupric Yersiniabactin Is a Virulence-Associated Superoxide Dismutase Mimic

Chaturvedi, Kaveri S.; Hung, Chia-Suei; Giblin, Daryl; Urushidani, Saki; Austin, Anthony M.; Dinauer, Mary C.; Henderson, Jeffrey P.

doi:10.1021/cb400658k

Cited by 94 publications

(115 citation statements)

References 72 publications

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“…These results suggest that the early stages of ascending UTI are competitive and favor isolates with specific siderophore profiles. In this and other studies, Ybt, a siderophore encoded by the Yersinia high pathogenicity island (HPI), was associated with urinary tract virulence (7,(13)(14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 64%

Enterobacteria secrete an inhibitor of Pseudomonas virulence during clinical bacteriuria

Ohlemacher¹,

Giblin²,

d’Avignon³

et al. 2017

Journal of Clinical Investigation

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Section: Introductionmentioning

confidence: 64%

Enterobacteria secrete an inhibitor of Pseudomonas virulence during clinical bacteriuria

Ohlemacher¹,

Giblin²,

d’Avignon³

et al. 2017

Journal of Clinical Investigation

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“…These studies establish that the sulfide-regulated repressor SqrR allows R. capsulatus to adapt to changes in sulfide availability via thiol persulfidation chemistry on SqrR, which we hypothesize involves the intermediacy of RSS in cells. The functional characteristics of SqrR appear largely analogous to those of CstR in S. aureus (26), which also regulates an H 2 S oxidation and detoxification system (46), despite vastly distinct structural scaffolds (47). Further elucidation of the functional role(s) of persulfide trafficking and sulfide homeostasis in R. capsulatus promises a better understanding of how these processes impact photosynthesis in this industrially important bacterium.…”

Section: Discussionmentioning

confidence: 99%

Sulfide-responsive transcriptional repressor SqrR functions as a master regulator of sulfide-dependent photosynthesis

Shimizu

Shen

Fang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

113

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Sulfide was used as an electron donor early in the evolution of photosynthesis, with many extant photosynthetic bacteria still capable of using sulfur compounds such as hydrogen sulfide (H 2 S) as a photosynthetic electron donor. Although enzymes involved in H 2 S oxidation have been characterized, mechanisms of regulation of sulfide-dependent photosynthesis have not been elucidated. In this study, we have identified a sulfide-responsive transcriptional repressor, SqrR, that functions as a master regulator of sulfide-dependent gene expression in the purple photosynthetic bacterium Rhodobacter capsulatus. SqrR has three cysteine residues, two of which, C41 and C107, are conserved in SqrR homologs from other bacteria. Analysis with liquid chromatography coupled with an electrospray-interface tandem-mass spectrometer reveals that SqrR forms an intramolecular tetrasulfide bond between C41 and C107 when incubated with the sulfur donor glutathione persulfide. SqrR is oxidized in sulfidestressed cells, and tetrasulfide-cross-linked SqrR binds more weakly to a target promoter relative to unmodified SqrR. C41S and C107S R. capsulatus SqrRs lack the ability to respond to sulfide, and constitutively repress target gene expression in cells. These results establish that SqrR is a sensor of H 2 S-derived reactive sulfur species that maintain sulfide homeostasis in this photosynthetic bacterium and reveal the mechanism of sulfide-dependent transcriptional derepression of genes involved in sulfide metabolism.sulfide sensor | photosynthesis regulation | reactive sulfur species | purple bacteria | Rhodobacter T he discovery of ∼550 deep-sea hydrothermal vents more than 30 y ago (1) has led to the theory that energy metabolism in early ancestral organisms may have arisen from deep-sea hydrothermal vents where simple inorganic molecules such as hydrogen sulfide or hydrogen gas, as well as methane, exist (2-4). Such ancient energy metabolism has been assumed to be similar to that of extant chemolithotrophs, which obtain energy from these molecules. Indeed, various chemolithoautotrophic microbes thrive in deep-sea hydrothermal vents and are capable of oxidizing sulfides, methane, and/or hydrogen gas for use as energy sources and electron donors (5). Some photosynthetic bacteria have also been isolated from deep-sea hydrothermal vents that can grow photosynthetically using sulfide as an electron donor and geothermal radiation as an energy source instead of solar radiation (6), as hypothesized for ancestral phototrophs.Many purple photosynthetic bacteria have remarkable metabolic versatility required to meet the energy demands of sulfidedependent and -independent photosynthesis as well as aerobic and anaerobic respiration. These bacteria tightly control the synthesis of their electron transfer proteins involved in each growth mode in response to a specific electron donor, oxygen tension, and light intensity (7, 8). Among these regulatory systems, oxygen-and lightsensing mechanisms have been well-studied; however, mechanisms used to sen...

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“…The yersiniabactin determinant is a well-known virulence factor responsible for copper binding (Chaturvedi et al 2014) that can be present in pathogens such as K. pneumoniae (Fodah et al 2014); Salmonella (Aviv et al 2014); E. coli (Schubert et al 2004), including the EHEC O104:H4 outbreak strain; and the highly virulent Yersinia pestis (Rakin et al 2012). Interestingly, several strains of Klebsiella and E. coli appear to have the sil/pco determinant in addition to the yersiniabactin synthesis cluster (Table 1).…”

Section: Yersiniabactin-not Just For Ironmentioning

confidence: 99%

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Hao

Lüthje

Qin

et al. 2015

Appl Microbiol Biotechnol

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The presence of metal resistance determinants in bacteria usually is attributed to geological or anthropogenic metal contamination in different environments or associated with the use of antimicrobial metals in human healthcare or in agriculture. While this is certainly true, we hypothesize that protozoan predation and macrophage killing are also responsible for selection of copper/zinc resistance genes in bacteria. In this review, we outline evidence supporting this hypothesis, as well as highlight the correlation between metal resistance and pathogenicity in bacteria. In addition, we introduce and characterize the Bcopper pathogenicity island^identified in Escherichia coli and Salmonella strains isolated from copper-and zinc-fed Danish pigs.

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Cupric Yersiniabactin Is a Virulence-Associated Superoxide Dismutase Mimic

Cited by 94 publications

References 72 publications

Enterobacteria secrete an inhibitor of Pseudomonas virulence during clinical bacteriuria

Enterobacteria secrete an inhibitor of Pseudomonas virulence during clinical bacteriuria

Sulfide-responsive transcriptional repressor SqrR functions as a master regulator of sulfide-dependent photosynthesis

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

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