Cupriavidus metallidurans NA4 actively forms polyhydroxybutyrate-associated uranium-phosphate precipitates

Rogiers, Tom; Merroun, Mohamed L.; Williamson, Adam; Leys, Natalie; Boon, Nico; Mijnendonckx, Kristel

doi:10.1016/j.jhazmat.2021.126737

Cited by 12 publications

(10 citation statements)

References 72 publications

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“…strain Y9602, respectively ( Beazley et al, 2007 ; Martinez et al, 2007 ). Another example is the association of uranium-phosphate minerals with polyhydroxybutyrate in Cupriavidus metallidurans NA4 ( Rogiers et al, 2021b ), which is resistant to uranium independent of the presence of phaC1 , encoding the poly(3-hydroxyalkanoate) polymerase subunit PhaC ( Rogiers, 2022 ). Therefore, other mechanisms could mediate resistance, including metal efflux systems as uranium toxicity is mainly exerted through its chemical metal-related properties.…”

Section: Metal Efflux Systemsmentioning

confidence: 99%

“…Although efflux systems are often upregulated after uranium exposure, a designated efflux system for uranium has not yet been identified. For instance, in C. metallidurans NA4 almost all genes (from nine different clusters) involved in the response to and detoxification of silver and copper were upregulated after uranium exposure ( Rogiers et al, 2021b ). In Chryseobacterium sp.…”

Section: Metal Efflux Systemsmentioning

confidence: 99%

“…strain PMSZPI, isolated from a uranium-enriched environment and in S. bentonitica BII-R7 czcA and czcA/cusA genes were upregulated after uranium exposure, respectively ( Nongkhlaw and Joshi, 2019 ; Pinel-Cabello et al, 2021 ). The expression of one of the two czc gene clusters present in C. metallidurans NA4 was also induced after exposure to uranium ( Rogiers et al, 2021b ). Three membrane fusion proteins and two outer membrane factors assisting HME-RND pumps were also upregulated in G. sulfurreducens after uranium induction, amongst which CzcC ( Orellana et al, 2014 ).…”

Section: Metal Efflux Systemsmentioning

confidence: 99%

“…In D. alaskensis G20, a cyclic AMP receptor protein (CRP) was found to possibly regulate expression of the mre operon for metal reduction, facilitating uranium reduction through thioredoxin ( Li and Krumholz, 2009 ). Finally, ten TCSs are upregulated in C. metallidurans after uranium induction of which five are known to be involved in metal resistance ( Rogiers et al, 2021b ). While extensive work has only been done in C. crescentus , there is currently little known on how uranium is sensed and how this results in the transcription of target genes.…”

Section: Regulatory Systemsmentioning

confidence: 99%

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Molecular Mechanisms Underlying Bacterial Uranium Resistance

Rogiers

Williamson²,

Leys³

et al. 2022

Front. Microbiol.

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Environmental uranium pollution due to industries producing naturally occurring radioactive material or nuclear accidents and releases is a global concern. Uranium is hazardous for ecosystems as well as for humans when accumulated through the food chain, through contaminated groundwater and potable water sources, or through inhalation. In particular, uranium pollution pressures microbial communities, which are essential for healthy ecosystems. In turn, microorganisms can influence the mobility and toxicity of uranium through processes like biosorption, bioreduction, biomineralization, and bioaccumulation. These processes were characterized by studying the interaction of different bacteria with uranium. However, most studies unraveling the underlying molecular mechanisms originate from the last decade. Molecular mechanisms help to understand how bacteria interact with radionuclides in the environment. Furthermore, knowledge on these underlying mechanisms could be exploited to improve bioremediation technologies. Here, we review the current knowledge on bacterial uranium resistance and how this could be used for bioremediation applications.

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Section: Metal Efflux Systemsmentioning

confidence: 99%

Section: Metal Efflux Systemsmentioning

confidence: 99%

Section: Metal Efflux Systemsmentioning

confidence: 99%

Section: Regulatory Systemsmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Mechanisms Underlying Bacterial Uranium Resistance

Rogiers

Williamson²,

Leys³

et al. 2022

Front. Microbiol.

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“…Gram-negative Cupriavidus metallidurans CH34 displays an extraordinary genomic plasticity that allowed it to evolve a unique combination of metabolic features, such as autotrophic growth on CO 2 as the sole carbon source combined with hydrogenotrophy; , degradation of BTEX (benzene, toluene, ethylbenzene, xylene); , high resistance to numerous metals; biomineralization of gold, platinum, silver and uranium; and accumulation of polyhydroxybutyrate . However, despite this exceptional potential as an industrial microbial chassis for applications such as bioremediation, biomining, biosensing, carbon sequestration, and bioprocessing, proof-of-concept studies investigating this microorganism for biotechnological applications are scarce and mostly limited to its use in the bioremediation of metal-contaminated soils and waters …”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34

et al. 2022

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Cupriavidus metallidurans CH34 exhibits extraordinary metabolic versatility, including chemolithoautotrophic growth; degradation of BTEX (benzene, toluene, ethylbenzene, xylene); high resistance to numerous metals; biomineralization of gold, platinum, silver, and uranium; and accumulation of polyhydroxybutyrate (PHB). These qualities make it a valuable host for biotechnological applications such as bioremediation, bioprocessing, and the generation of bioelectricity in microbial fuel cells (MFCs). However, the lack of genetic tools for strain development and studying its fundamental physiology represents a bottleneck to boosting its commercial applications. In this study, inducible and constitutive promoter libraries were built and characterized, providing the first comprehensive list of biological parts that can be used to regulate protein expression and optimize the CRISPR-Cas9 genome editing tools for this host. A single-plasmid CRISPR-Cas9 system that can be delivered by both conjugation and electroporation was developed, and its efficiency was demonstrated by successfully targeting the pyrE locus. The CRISPR-Cas9 system was next used to target candidate genes encoding type IV pili, hypothesized by us to be involved in extracellular electron transfer (EET) in this organism. Single and double deletion strains (ΔpilA, ΔpilE, and ΔpilAE) were successfully generated. Additionally, the CRISPR-Cas9 tool was validated for constructing genomic insertions (ΔpilAE::gf p and ΔpilAE::λ Pr gfp). Finally, as type IV pili are believed to play an important role in extracellular electron transfer to solid surfaces, C. metallidurans CH34 ΔpilAE was further studied by means of cyclic voltammetry using disposable screen-printed carbon electrodes. Under these conditions, we demonstrated that C. metallidurans CH34 could generate extracellular currents; however, no difference in the intensity of the current peaks was found in the ΔpilAE double deletion strain when compared to the wild type. This finding suggests that the deleted type IV pili candidate genes are not involved in extracellular electron transfer under these conditions. Nevertheless, these experiments revealed the presence of different redox centers likely to be involved in both mediated electron transfer (MET) and direct electron transfer (DET), the first interpretation of extracellular electron transfer mechanisms in C. metallidurans CH34.

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