Cupriavidus agavae sp. nov., a species isolated from Agave L. rhizosphere in northeast Mexico

Abstract

During the isolation of bacteria from the Agave L. rhizosphere in northeast Mexico, four strains with similar BOX-PCR patterns were collected. The 16S rRNA gene sequences of all four strains were very similar to each other and that of the type strains of Cupriavidus metallidurans CH34T (98.49 % sequence similarity) and Cupriavidus necator N-1T (98.35 %). The genome of strain ASC-9842T was sequenced and compared to those of other Cupriavidus species. ANIb and ANIm values with the most closely related species were lower than 95%, while the in silico DNA–DNA hybridization values were also much lower than 70 %, consistent with the proposal that they represent a novel species. This conclusion was supported by additional phenotypic and chemotaxonomic analyses. Therefore, the name Cupriavidus agavae sp. nov. is proposed with the type strain ASC-9842T (=LMG 26414T=CIP 110327T).

Cupriavidus cauae sp. nov., isolated from blood of an immunocompromised patient

“…The genus Cupriavidus includes 19 validly published taxa, with C. necator reported as the type species [3], including the most recently described species, Cupriavidus agavae [4]. Cupriavidus species are found in the soil, rhizosphere and in human clinical specimens, particularly in samples obtained from debilitated patients [1, 4–9].…”

“…The genus Cupriavidus was first proposed in 1987 by Makkar and Casida, who described a non-obligate bacterial predator in soil, named Cupriavidus necator N-1 T [2]. The genus Cupriavidus includes 19 validly published taxa, with C. necator reported as the type species [3], including the most recently described species, Cupriavidus agavae [4]. Cupriavidus species are found in the soil, rhizosphere and in human clinical specimens, particularly in samples obtained from debilitated patients [1,[4][5][6][7][8][9].…”

“…The genus Cupriavidus includes 19 validly published taxa, with C. necator reported as the type species [3], including the most recently described species, Cupriavidus agavae [4]. Cupriavidus species are found in the soil, rhizosphere and in human clinical specimens, particularly in samples obtained from debilitated patients [1,[4][5][6][7][8][9]. Of all the Cupriavidus species, Cupriavidus gilardii has been most frequently isolated from clinical specimens, including blood, throat, abscess and/or stool specimens [7][8][9].…”

The multi metal-resistant bacterium Cupriavidus metallidurans CH34 affects growth and metal mobilization in Arabidopsis thaliana plants exposed to copper

“…C. metallidurans' ability to act as a PGPR (see the increase in root length at low Cu 2+ levels, Fig. 1), at least in A. thaliana, is not surprising as several members of the Cupriavidus genus and the Burkholderiaceae family are well-known PGPRs or, at least, interact with plants (Gyaneshwar et al, 2011;Bhattacharyya & Jha, 2012;Poupin et al, 2013;Arroyo-Herrera et al, 2020). Because PGPR traits (Zeffa et al, 2020), including 1-aminocyclopropane-1-carboxylate deaminase, auxin phytohormone production, phosphate solubilization, and nitrogen fixation (Bhattacharyya & Jha, 2012;Aeron et al, 2020), have not yet been reported in C. metallidurans, new PGPR traits would be interesting to explore in this bacterium.…”