Cumulus contributions during bovine fertilization in vitro

Tanghe, Sofie; Soom, Ann Van; Mehrzad, Jalil; Maes, Dominiek; Duchateau, Luc; Kruif, Aart de

doi:10.1016/s0093-691x(02)01360-2

Cited by 85 publications

(64 citation statements)

References 49 publications

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“…While it is not clear exactly how cumulus cells support oocyte maturation, it is generally accepted that cumulus-oocyte communication via an intact corona radiata (Ge et al 2008) is necessary for immature oocytes to resume meiosis and attain full cytoplasmic maturation and developmental competence ( Vanderhyden & Armstrong 1989, Tanghe et al 2003, and that gap junctions play an important role in this communication (Shimada et al 2001). Gap junctions are channels between adjoining cells that allow the passage of molecules less than 1 kDa in size, such as ions, nutrients and metabolites (Gilula et al 1972).…”

Section: Discussionmentioning

confidence: 99%

“…MAP kinase, MPF: Bogliolo et al 2007). It has also been proposed that damage to the cumulus investment plays a role in compromised developmental competence since freezing and thawing damages cumulus cells surrounding mouse oocytes (Ruppert-Lingham et al 2003) and the gap junctions between equine oocytes and their surrounding cumulus-corona radiata cells (Hochi et al 1996), where the interaction between oocyte and cumulus has been shown to be critical to successful nuclear and cytoplasmic maturation for at least bovine oocytes (Zhang et al 1995, Hassan 2001, Tanghe et al 2003. While Bogliolo et al (2007) reported a counterintuitive improvement in survival and meiotic competence for immature sheep oocytes denuded of their cumulus prior to vitrification, they also noted the depletion of enzymes (MPF and MAPK) required to support embryo development.…”

Section: Introductionmentioning

confidence: 99%

“…However, cumulus cell secretions and gap junctional contact between the oocyte and corona radiata cells have been reported to promote sperm penetration and thereby improve fertilisation rates in conventional IVF systems for both cattle ( Tanghe et al 2003) and cats (Godard et al 2009). More controversially, Imoedemhe & Sigue (1992) reported that the cumulus investment protected MII oocytes against structural damage during cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

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Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Tharasanit

Colleoni²,

Galli³

et al. 2009

Reproduction

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Vitrifying oocytes is a potentially valuable means of preserving the female germ line, but significantly compromises oocyte developmental competence. This study examined the hypothesis that the cumulus complex protects the oocyte during vitrification. Vitrified-warmed immature cumulus oocyte complexes (COCs) were labelled with a plasma membrane impermeant DNA marker (ethidium homodimer-1) to examine the percentage and location of dead cumulus cells, and to investigate the effect of the proportion of dead cells (C1,C2 or C3) on the success of in vitro maturation (IVM). Further, oocytes were labelled for connexin-43 or injected with Lucifer yellow dye to determine whether the integrity of the gap junctions between an oocyte and its cumulus was compromised by vitrification. Finally, the effect of denuding immature and mature oocytes on their ability to withstand vitrification was examined. Cryopreserving immature COCs increased the number of dead cumulus cells (13 vs 2.6% for controls; P!0.05). However, an increased proportion of dead cumulus cells did not affect post-warming maturation rates (w30% MII ) presumably because dead cells were located at the periphery of the cumulus mass and cumulus-oocyte gap junction communication was not disrupted. Moreover, cumulus removal prior to IVM or vitrification indicated that while the cumulus does protect immature oocytes during vitrification it does so by mechanisms other than support during maturation. Cumulus presence was also found to protect mature equine oocytes against vitrification-induced damage since cumulus-enclosed MII oocytes preserved their meiotic spindle quality better during vitrification than denuded oocytes (38.1 vs 3.1% normal spindles; P!0.05).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Tharasanit

Colleoni²,

Galli³

et al. 2009

Reproduction

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“…In the sites of contact, cumulus cells communicate with the oocyte by means of gap junctions [35]. Gap junctions appear to be critical for the transport of small size [less than 1 kDa] cumulus-derived factors, metabolites and signalling molecules that are necessary for immature oocytes to resume meiosis and acquire full cytoplasmic maturation and developmental competence [36][37][38]. Moreover, TZPs have a role in maintaining the GV or the meiotic spindle in the correct position in the cortex during oocyte maturation [39][40][41].…”

Section: Oocyte-cumulus Cell Contact and Interactionmentioning

confidence: 99%

The current challenges to efficient immature oocyte cryopreservation

Brambillasca

Guglielmo

Coticchio

et al. 2013

J Assist Reprod Genet

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Oocyte cryopreservation represents an important tool for assisted reproductive technology. It offers the opportunity to preserve fertility in women at risk of loss of the ovarian function for various pathologies. It also represents a treatment alternative for couples that cannot benefit from embryo cryopreservation because of moral, religious, or legal constrains. On the other hand, in vitro oocyte maturation has a range of applications. It can be applied in patients with a contraindication to ovarian stimulation to prevent ovarian hyperstimulation syndrome or to eliminate the risk of stimulation of hormone-sensitive tumours in cancer patients. However, while mature oocyte cryopreservation has found widespread application and oocyte in vitro maturation has a place for the treatment of specific clinical conditions, data on the efficiency of freezing of immature or in vitro matured oocytes are poorer. In this review we will focus on the combination of oocyte in vitro maturation with oocyte cryopreservation with particular emphasis on the biological implications of the cryopreservation of immature or in vitro matured oocytes. The two cryopreservation approaches, slow freezing and vitrification, will be discussed in relation to possible cryodamage occurring to subcellular structures of the oocyte and the functional interaction between oocyte and cumulus cells.

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“…Ovaries and oocytes were collected as described by Tanghe et al (2003). After collection, ovaries were rinsed in physiological saline (0.9% NaCl) with 0.5% kanamycin.…”

Section: In Vitro Production Of Embryosmentioning

confidence: 99%

Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro

Leroy¹,

Vanholder²,

Mateusen³

et al. 2005

Reproduction

417

356

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In this study concentration and composition of non-esterified fatty acids (NEFA) in follicular fluid (FF) of high-yielding dairy cows were determined during the period of negative energy balance (NEB) early post partum. NEFA were then added during in vitro maturation at concentrations measured previously in FF to evaluate their effect on the oocyte's developmental competence. At 16 and 44 days post partum, FF of the dominant follicle and blood were collected from nine high-yielding dairy cows. Samples were analysed for NEFA concentration and composition. NEFA concentrations in FF (0.2 -0.6 mmol/l) during NEB remained 6 40% lower compared with serum (0.4 -1.2 mmol/l). The NEFA composition differed significantly between serum and FF with oleic acid (OA), palmitic acid (PA) and stearic acid (SA) being the predominant fatty acids in FF. Based on these results, 5115 oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of 0.200 mmol/l OA, 0.133 mmol/l PA or 0.067 mmol/l SA dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in SOF medium. Addition of PA or SA during oocyte maturation had negative effects on maturation, fertilization and cleavage rate and blastocyst yield. More (late) apoptotic cumulus cells were observed in cumulus -oocyte complexes matured in the presence of SA or PA. Ethanol or OA had no effect. These in vitro results suggest that NEB may hamper fertility of high-yielding dairy cows through increased NEFA concentrations in FF affecting oocyte quality. Reproduction (2005) 130 485-495

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Cumulus contributions during bovine fertilization in vitro

Cited by 85 publications

References 49 publications

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

Protective effects of the cumulus-corona radiata complex during vitrification of horse oocytes

The current challenges to efficient immature oocyte cryopreservation

Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro

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