Cumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass

Hong, Sihui; Chiu, Philip C.N.; Lee, Ckf; Tse, Jenny; Ho, Pak‐Chung; Yeung, William S.B.

doi:10.1016/j.fertnstert.2008.07.1760

Cited by 33 publications

(31 citation statements)

References 59 publications

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“…In particular, in a video microscopic in vitro fertilization experiment, it was recently found that double transgenic male mice spermatozoa, expressing enhanced green fluorescent protein (EGFP) in their acrosomes and red fluorescent protein (Ds-Red2) in their midpiece, undergo AR before reaching the ZP ( Jin et al, 2011). In other words, it was shown that AR occurs during the interaction with cumulus and it is not necessary to penetrate ZP, thus confirming the role of receptors for cumulus extracellular matrix proteins observed in spermatozoa of several species (Barboni et al, 2001;Gadella, 2012;Hong et al, 2009). Furthermore, recently, the concept that the spermatozoa that have already undergone AR are unable to bind the ZP and have lost their fertilizing ability has been revamped.…”

Section: Introductionmentioning

confidence: 62%

Network Analyses of Sperm–Egg Recognition and Binding: Ready to Rethink Fertility Mechanisms?

Bernabò

Ordinelli

Agostino

et al. 2014

OMICS: A Journal of Integrative Biology

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The rapid growth of published literature makes biomedical text mining increasingly invaluable for unpacking implicit knowledge hidden in unstructured text. We employed biomedical text mining and biological networks analyses to research the process of sperm egg recognition and binding (SERB). We selected from the literature the molecules expressed either on spermatozoa or on oocytes thought to be involved in SERB and, using an automated literature search software (Agilent Literature Search), we realized a network, SERBN, characterized by a hierarchical scale free and a small world topology. We used an integrated approach, either based on selection of hubs or by a cluster analysis, to discern the key molecules of SERB. We found that in most cases some of them are not directly situated on spermatozoa and oocyte, but are dispersed in oviductal fluid or embedded in exosomes present in the perivitelline space. To confirm and validate our results, we performed further analyses using STRING and Reactome FI software. Our findings underscore that the fertility is not a property of gametes in isolation, but rather depends on the functional integrity of the entire reproductive system. These observations collectively underscore the importance of integrative biology in exploring biological systems and in rethinking of fertility mechanisms in the light of this innovative approach.

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Section: Introductionmentioning

confidence: 62%

Network Analyses of Sperm–Egg Recognition and Binding: Ready to Rethink Fertility Mechanisms?

Bernabò

Ordinelli

Agostino

et al. 2014

OMICS: A Journal of Integrative Biology

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“…In the presence of human cumulus oophorus, a significant enhancement of human sperm AR was observed after 5 h preincubation with the capacitation medium in vitro (Tesarík, 1985). A study examining the effect of cumulus matrix and cumulus cells on sperm penetration revealed that, compared to cell-depleted matrix, more sperm could penetrate the intact cumulus complex, with a higher percentage of normal morphology, AR and motility (Hong et al, 2009). Fragments of preovulatory human cumulus were washed 3 times and incubated for 24 h, and the spent media and washes were assayed for their ability to initiate human sperm AR in vitro.…”

Section: Facilitated Ar In the Presence Of Cumulus Layermentioning

confidence: 99%

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

2011

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Fertilization is a process involving multiple steps that lead to the final fusion of one sperm and the oocyte to form the zygote. One of the steps, acrosome reaction (AR), is an exocytosis process, during which the outer acrosome membrane fuses with the inner sperm membrane, leading to the release of acrosome enzymes that facilitate sperm penetration of the egg investments. Though AR has been investigated for decades, the initial steps of AR in vivo, however, remain largely unknown. A well elucidated model holds the view that AR occurs on the surface of the zona pellucida (ZP), which is triggered by binding of sperm with one of the ZP glycosylated protein, ZP3. However, this model fails to explain the large number of 'falsely' acrosome-reacted sperms found within the cumulus layer in many species examined. With the emerging evidence of cross-talk between sperm and cumulus cells, the potential significance of AR in the cumulus oophorus, the outer layer of the egg, has been gradually revealed. Here we review the acrosome status within the cumulus layer, the cross-talk between sperm and cumulus cells with the involvement of a novel spermreleased factor, NYD-SP8, and re-evaluate the importance and physiological significance of the AR in the cumulus in fertilization.

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“…The acquisition of hyperactivation is an absolute requirement for penetration of the cumulus, extracellular matrix and the ZP (41). Sperm able to traverse the cumulus oophorus have higher percentages of normal morphology and specific motility patterns, and this selection is aided both by the cumulus matrix and the cumulus cells (42). Moreover, sperm capable of binding to hyaluronic acid, the main component of the cumulus extracellular matrix, are mature, acrosome intact (43), and have a higher DNA integrity (44).…”

Section: Cumulus Oophorusmentioning

confidence: 99%

Sperm selection before intracytoplasmic sperm injection

Talevi¹

2015

cce

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SummaryIn assisted reproduction techniques (ART), the success of embryo development depends mostly on the quality of gametes, and pregnancy outcome is strictly related to both the oocyte and the fertilizing sperm quality. Therefore, during ART, it is mandatory to select a mature and viable sperm with high DNA integrity. Routine sperm preparation and selection in ART is based on density gradient centrifugation or motility. However, it is becoming increasingly clear that new and more rigorous procedures should be considered to recover those sperm able to properly support fertilization, embryo development and birth of healthy babies.

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Cumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass

Abstract: Both the cumulus matrix and the cumulus cells contribute to the effect of cumulus oophorus on spermatozoa penetrating through it.

Cited by 33 publications

References 59 publications

Network Analyses of Sperm–Egg Recognition and Binding: Ready to Rethink Fertility Mechanisms?

Network Analyses of Sperm–Egg Recognition and Binding: Ready to Rethink Fertility Mechanisms?

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

Sperm selection before intracytoplasmic sperm injection

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