Cumulative pregnancy rates resulting from the use of fresh and frozen oocytes: 7 years’ experience

Borini, Andrea; Lagalla, Cristina; Bonu, Maria Antonietta; Bianchi, Veronica; Flamigni, Carlo; Coticchio, Giovanni

doi:10.1016/s1472-6483(10)62002-0

Cited by 100 publications

(44 citation statements)

References 34 publications

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“…Slow-freezing protocols have been mainly studied by Italian researchers. Favourable success rates have been reported, with an increasing number of pregnancies recorded over the years [9][10][11][12][13][14][15][16][17].…”

Section: Techniques For Oocyte Cryopreservationmentioning

confidence: 99%

The current challenges to efficient immature oocyte cryopreservation

Brambillasca

Guglielmo

Coticchio

et al. 2013

J Assist Reprod Genet

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Oocyte cryopreservation represents an important tool for assisted reproductive technology. It offers the opportunity to preserve fertility in women at risk of loss of the ovarian function for various pathologies. It also represents a treatment alternative for couples that cannot benefit from embryo cryopreservation because of moral, religious, or legal constrains. On the other hand, in vitro oocyte maturation has a range of applications. It can be applied in patients with a contraindication to ovarian stimulation to prevent ovarian hyperstimulation syndrome or to eliminate the risk of stimulation of hormone-sensitive tumours in cancer patients. However, while mature oocyte cryopreservation has found widespread application and oocyte in vitro maturation has a place for the treatment of specific clinical conditions, data on the efficiency of freezing of immature or in vitro matured oocytes are poorer. In this review we will focus on the combination of oocyte in vitro maturation with oocyte cryopreservation with particular emphasis on the biological implications of the cryopreservation of immature or in vitro matured oocytes. The two cryopreservation approaches, slow freezing and vitrification, will be discussed in relation to possible cryodamage occurring to subcellular structures of the oocyte and the functional interaction between oocyte and cumulus cells.

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Section: Techniques For Oocyte Cryopreservationmentioning

confidence: 99%

The current challenges to efficient immature oocyte cryopreservation

Brambillasca

Guglielmo

Coticchio

et al. 2013

J Assist Reprod Genet

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“…However, for almost two decades the endeavor to preserve oocytes was frustrated. Since the poor implantation and pregnancy rate severely limited the clinical application of oocytes cryopreservation, the embryos cryopreservation has developed as a preferred choice of fertility preservation, though they have the similar survival and fertilization rate [1][2][3]. In recent years, vitrification cryopreservation developed rapidly, and many clinical reviews supported that the oocytes from vitrification/ thawing cycles could result in similar clinical outcomes with fresh oocytes [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes

Han¹,

Liu²,

Wang

et al. 2012

J Assist Reprod Genet

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“…Controlled ovarian hyperstimulation was induced with long protocols using GnRH agonist and recombinant FSH, according to the standard clinical procedures routinely employed by the participating clinics (Borini et al, 2006a;De Santis et al, 2007). Ten thousand IU of HCG were administered 36 h prior to oocyte collection.…”

Section: Source Of Oocytesmentioning

confidence: 99%

“…These experiences have principally resulted from the application of controlled rate freezing (slow cooling) protocols (Borini et al, 2004(Borini et al, ,2006aChen et al, 2005;Boldt et al, 2006;Levi Setti et al, 2006), although the potential of vitrification technology, still largely unexplored from a clinical standpoint, has recently gained the attention of cryobiologists and IVF specialists (Kuwayama et al, 2005;Lucena et al, 2006). Newly introduced protocols seem to have met the fundamental need of improving post-thaw survival rates, previously unacceptably low, but clinical experience acquired so far is in fact either contradictory or numerically insufficient to confirm that the oocyte developmental potential may be preserved substantially unaltered after cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

Permeability of human oocytes to ethylene glycol and their survival and spindle configurations after slow cooling cryopreservation

Santis

Coticchio²,

Paynter

et al. 2007

Human Reproduction

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BACKGROUND: To develop novel cryopreservation methods, we estimated the permeability coefficients Lp (hydraulic conductivity) and P EG (cryoprotectant permeability) of mature human oocytes after exposure to ethylene glycol (EG) and tested the efficiency of a multi-step slow cooling protocol based on this cryoprotectant. METHODS: Oocytes were perfused with 1.5 mol/l EG for 10 min. Oocyte volume at each time point was calculated and normalized to the original volume. Slow cooling was conducted by exposing oocytes to increasing EG concentrations (0.5, 1.0 and 1.5 mol/l n 5 155) or 1.5 mol/l of propane-1,2-diol (PrOH) n 5 102. Oocytes which survived cryopreservation n 5 80 and fresh oocytes n 5 73 were prepared for confocal microscopy analysis of the meiotic spindle. RESULTS: During EG exposure, oocytes underwent an abrupt 50% volume reduction. Complete recovery of the initial volume was not observed. From the values of a best fit plot, the coefficients Lp 5 0.82 + + + + + 0.29 mm min 21 atm 21 (mean + + + + + SD) and P EG 0.10 + + + + + 0.01 mm s 21 were generated. Survival rates after freezing with EG were lower than with PrOH (51.6 versus 71.5%, respectively, P < 0.05). The frequencies of normal spindle configuration were lower in frozen EG and frozen PrOH oocytes compared with fresh oocytes (53.8, 50.9 and 66.7%, respectively, P < 0.05). CONCLUSIONS: The oocyte plasmalemma possesses limited permeability to EG and EG exposure causes considerable osmotic stress. However, post-thaw rates of survival and normal meiotic spindle organization may be preserved by protocols which are designed in order to minimize osmotic stress.

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Cumulative pregnancy rates resulting from the use of fresh and frozen oocytes: 7 years’ experience

Cited by 100 publications

References 34 publications

The current challenges to efficient immature oocyte cryopreservation

The current challenges to efficient immature oocyte cryopreservation

Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes

Permeability of human oocytes to ethylene glycol and their survival and spindle configurations after slow cooling cryopreservation

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