Cumulative effects of road de-icing salt on amphibian behavior

Denoël, Mathieu; Bichot, Marion; Ficetola, Gentile Francesco; Delcourt, Johann; Ylieff, Michel; Kestemont, Patrick; Poncin, Pascal

doi:10.1016/j.aquatox.2010.05.007

Cited by 79 publications

(60 citation statements)

References 38 publications

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“…Behavioral markers can now be rigorously quantified in experiments that can be replicated and thus are powerful tools to complement other traditional survival analyses. New developments of analytical methods of behaviors, such as image analysis (Friberg-Jensen et al 2010) and video-tracking of locomotor patterns (Denoël et al 2010;Winandy and Denoël 2011), but also analyses of more complex aspects of behaviors such as sensory perception (Mandrillon and Saglio 2007) and learning processes (Eddins et al 2010), are encouraged because of the complex and often overlooked actions of chemicals. Going in-depth with such techniques would also put behavioral assessment closer to physiological, histological and molecular studies in explaining the mechanisms of toxicity and the action of chemicals at very low concentrations.…”

Section: Concentrationsmentioning

confidence: 99%

“…after the daily observations. This food resource is typical in laboratory experiments on ranid tadpoles and has the advantage of being easily quantified, particularly for behavioral observations (Denoël et al 2010). Photoperiod followed the natural cycle of the capture place, i.e.…”

Section: Field and Laboratory Maintenancementioning

confidence: 99%

“…Behavioral ecotoxicology is an emerging field providing multiple ways to quantify responses from contaminated organisms (Bromhall 2005;Denoël et al 2010;Giusi et al 2010). Studying behavioral endpoints is particularly relevant because they could link varied physiological disorders with ecological processes and because they could be exhibited before other traits, with possible direct consequences on the organisms (Tu et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Using sets of behavioral biomarkers to assess short-term effects of pesticide: a study case with endosulfan on frog tadpoles

Denoël¹,

D’Hooghe²,

Ficetola

et al. 2012

Ecotoxicology

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Pesticides and other chemicals often have detrimental effects at environmental concentrations. Many amphibian species are particularly threatened because of their susceptibility but also because wetlands are often polluted. Behavioral assessments of toxicity have the advantage of showing sublethal effects but quantitative measures at varied scales of integrations are rarely considered together. In this study, we aimed at showing that these behavioral endpoints could be differently affected across time and concentrations, and be biomarkers of toxicity. To this end, we tested the effects of an organochlorine pesticide (endosulfan) on amphibians during a standard 96 h test. We evaluated possible lag effects in continuing the analyses after removal of the pesticide. The study was based on 240 tadpoles (4 pesticide treatments: 0.4, 3, 22, and 282 μg/l, 1 control and 1 solvent-control). Abnormal behaviors such as lying and swirling rapidly were exhibited only in the presence of the pesticide. Essential functions such as breathing and feeding were deeply affected by the pesticide: contaminated tadpoles breathed and fed less than control tadpoles. They also moved less and occupied a more central position in the aquariums in the presence of the pesticide. A higher mortality was only found at the highest concentration. These results suggest that endosulfan is toxic to amphibians at environmental concentrations. Behavioral markers showed potential as early warning systems. They should thus be used in complement to other markers to detect sublethal effects only a few days after application of the pesticide and at concentrations where mortality does not occur.

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Section: Concentrationsmentioning

confidence: 99%

Section: Field and Laboratory Maintenancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Using sets of behavioral biomarkers to assess short-term effects of pesticide: a study case with endosulfan on frog tadpoles

Denoël¹,

D’Hooghe²,

Ficetola

et al. 2012

Ecotoxicology

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“…Although video tracking to measure aquatic vertebrates' behaviors is frequently used in pharmacology, in toxicology, and in behavioral ontogeny, it remains quite rare in ecological studies (i.e., Denoel et al, 2010;Peake, Matos, & McGregor, 2006). Moreover, tracking a transparent animal with a water current in the dark is a challenge.…”

Section: −1mentioning

confidence: 99%

Video tracking in the extreme: A new possibility for tracking nocturnal underwater transparent animals with fluorescent elastomer tags

et al. 2011

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Initially developed so that an individual could be recognized in mark-recapture studies of aquatic animals, fluorescent visible implant elastomer (VIE) tags are used here for a new application in ethometry: the study of the behavior of transparent animals in dim light or in darkness using automatic tracking technology. The application and validation of this multitracking method is tested in the context of research on the estuarine migratory behavior of the glass eel (Anguilla anguilla), a crucial point to better understand the dynamics of this endangered species. The method makes it possible to measure the activity (notably the distance and speed) of four individuals as a function of tidal and nycthemeral rhythms in the same flume (a circular aquarium simulating river or estuarine conditions) across a wide time scale (from seconds to weeks).

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“…To avoid a too large number of descriptors with respect to sample size, we did not include additional fixed effects. We also added body length (log 10 transformed) as covariate in our analyses to control for variation in body size between individuals (see Denoël et al 2010). Because this variable had no significant effect on the studied behavioural patterns and because it is recommended to avoid a large number of descriptors with respect to our sample size, the final linear models presented here do not include the covariate.…”

Section: Statistical Analysesmentioning

confidence: 99%

Out of the ground: two coexisting fossorial toad species differ in their emergence and movement patterns

Székely

Cogălniceanu

Székely

et al. 2017

Zoology

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Understanding the way species with similar niches can coexist is a challenge in ecology. The niche partitioning hypothesis has received much support, positing that species can exploit available resources in different ways. In the case of secretive species, behavioural mechanisms of partitioning are still poorly understood. This is especially true for fossorial frogs because individuals hide underground by day and are active only during the night. We investigated the nocturnal activity and tested the niche partitioning hypothesis in two syntopic fossorial spadefoot toads (Pelobates fuscus and P. syriacus) by examining interspecific variation in emergence from the soil. We employed a night vision recording system combined with video-tracking analyses in a replicated laboratory setting to quantify individual movement patterns, a procedure that has not been used until now to observe terrestrial amphibians. Most individuals appeared on the surface every night and returned to their original burrow (about 60% of the times), or dug a new one around morning. There was a large temporal overlap between the two species. However, P. syriacus was significantly more active than P. fuscus in terms of total distance covered and time spent moving, while P. fuscus individuals left their underground burrow more frequently than P. syriacus. Consequently, P. fuscus adopted more of a sit-and-wait behaviour compared to P. syriacus, and this could facilitate their coexistence. The use of night video-tracking technologies offered the advantage of individually tracking these secretive organisms during their nocturnal activity period and getting fine-grain data to understand their movement patterns.

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Cumulative effects of road de-icing salt on amphibian behavior

Cited by 79 publications

References 38 publications

Using sets of behavioral biomarkers to assess short-term effects of pesticide: a study case with endosulfan on frog tadpoles

Using sets of behavioral biomarkers to assess short-term effects of pesticide: a study case with endosulfan on frog tadpoles

Video tracking in the extreme: A new possibility for tracking nocturnal underwater transparent animals with fluorescent elastomer tags

Out of the ground: two coexisting fossorial toad species differ in their emergence and movement patterns

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