Culturing of cells as influenced by exposure to AC and DC fields

Abdelsalam, Mohamed; Nakano, Michihiko; Tanino, Masakazu; Mizuno, Akira

doi:10.1088/1742-6596/142/1/012051

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…At the meantime, corona discharges used in electrostatic precipitators, ESPs, generate radicals and other oxidative particles. ( 10 ) Those are effective to destroy BPs. Therefore, ESPs are potentially an important device to control airborne BPs, especially in hospitals and in public spaces.…”

Section: Collection and Destruction Of Bps By Electrostatic Precipitamentioning

confidence: 99%

Destruction of biological particles using non-thermal plasma

Mizuno

2017

J. Clin. Biochem. Nutr.

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Mechanism of inactivation of bio-particles exposed to non-thermal plasma (NTP), namely, dielectric barrier discharge (DBD), and plasma jet (PJ), has been studied using E. coli, B. subtilis spore, S. cerevisiae and bacteriophages. States of different biological components were monitored during the course of inactivation. Analysis of green fluorescent protein, GFP, introduced into E. coli. or B. subtiles spore cells proved that radicals generated by NTP penetrate into microbes, destroying the cell membrane and finally damage the genes. We have evaluated the damage of the bacteriophages. Bacteriophage λ having double stranded DNA was exposed to DBD, then DNA was purified and subjected to in vitro DNA packaging reactions. The re-packaged phages consist of the DNA from discharged phages and brand-new coat proteins were proved to be active, indicating that the damage of coat proteins is responsible for inactivation. M13 phages having single stranded DNA were also examined with the same manner. In this case, damage to the DNA was as severe as that of the coat proteins. For practical applications, DBD showed very intense sterilization ability for B. Subtilis spore with the D-value of less than 10 s. This result indicates a possibility of application of NTP for quick sterilization.

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Section: Collection and Destruction Of Bps By Electrostatic Precipitamentioning

confidence: 99%

Destruction of biological particles using non-thermal plasma

Mizuno

2017

J. Clin. Biochem. Nutr.

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Novel Virus Air Sampler Based on Electrostatic Precipitation and Air Sampling of SARS-CoV-2

et al. 2023

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The assessment of airborne viruses in air is a critical step in the design of appropriate prevention and control measures. Hence, herein, we developed a novel wet-type electrostatic air sampler using a viral dissolution buffer containing a radical scavenging agent, and verified the concentration of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in the air of hospital rooms inhabiting coronavirus disease 2019 (COVID-19) patients and public areas. RNA damage caused by corona discharge was negligible when Buffer AVL was used as the collecting electrode. The viral RNA concentration in the air of the room varied by patient: 3.9 × 103 copy/m3 on the 10th day after onset in a mild case and 1.3 × 103 copy/m3 on the 18th day in a severe case. Viral RNA levels were 7.8 × 102 and 1.9 × 102 copy/m3 in the air of the office and food court, respectively, where people removed their masks when eating and talking, but it remained undetected in the station corridor where all the people were wearing masks. The assessment of airborne SARS-CoV-2 RNA using the proposed sampler can serve as a basis for the safe discontinuation of COVID-19 isolation precautions to identify exposure hotspots and alert individuals at increased infection risks.

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Culturing of cells as influenced by exposure to AC and DC fields

Cited by 2 publications

References 6 publications

Destruction of biological particles using non-thermal plasma

Destruction of biological particles using non-thermal plasma

Novel Virus Air Sampler Based on Electrostatic Precipitation and Air Sampling of SARS-CoV-2

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