Culturing conditions remarkably affect viability and organization of mouse subventricular zone in ex vivo cultured forebrain slices

Armentano, Maria Francesca; Canalia, N.; Crociara, Paola; Bonfanti, Luca

doi:10.1016/j.jneumeth.2011.01.031

Cited by 3 publications

(2 citation statements)

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“…The density of dividing cells revealed by Ki-67 antigen localization in the SVZ-lr of the neonatal dolphins (43,16±32,92) is 34 fold lower when compared with the germinal layer of the cerebellar cortex in the same animals (1504,63±374; Figs. 3 and 5 and Table 4), 62 fold lower than that existing in the SVZ of neonatal rodents (2657±86; Armentano et al, 2011), 47 fold lower than in adult rodents (2018,5±420; Rolando et al, 2012; Fig. 5), and it is not higher than in the surrounding brain parenchyma (Fig.…”

Section: Discussionmentioning

confidence: 81%

Non-neurogenic SVZ-like niche in dolphins, mammals devoid of olfaction

2017

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Adult neurogenesis has been implicated in brain plasticity and brain repair. In mammals, it is mostly restricted to specific brain regions and specific physiological functions. The function and evolutionary history of mammalian adult neurogenesis has been elusive so far. The largest neurogenic site in mammals (subventricular zone, SVZ) generates neurons destined to populate the olfactory bulb. The SVZ neurogenic activity appears to be related to the dependence of the species on olfaction since it occurs at high rates throughout life in animals strongly dependent on this function for their survival. Indeed, it dramatically decreases in humans, who do not depend so much on it. This study investigates whether the SVZ neurogenic site exists in mammals devoid of olfaction and olfactory brain structures, such as dolphins. Our results demonstate that a small SVZ-like region persists in these aquatic mammals. However, this region seems to have lost its neurogenic capabilities since neonatal stages. In addition, instead of the typical newly generated neuroblasts, some mature neurons were observed in the dolphin SVZ. Since cetaceans evolved from terrestrial ancestors, non-neurogenic SVZ may indicate extinction of adult neurogenesis in the absence of olfactory function, with the retention of an SVZ-like anatomical region either vestigial or of still unknown role.

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Section: Discussionmentioning

confidence: 81%

Non-neurogenic SVZ-like niche in dolphins, mammals devoid of olfaction

2017

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“…However, this method should be approached with some caution if it is to be used to address questions about how certain molecules may influence migration patterns. Bonfanti and colleagues have shown that culturing of SVZ containing coronal sections results in significant disruption of slice cytoarchitecture and cell death over the first several days (Armentano et al, 2011). As a consequence of this loss of 3D structure, neuroblasts disaggregate at the top and bottom of the slice.…”

Section: Technical Considerations When Setting Up a Two-photon Microsmentioning

confidence: 99%

Subventricular Zone Cell Migration: Lessons from Quantitative Two-Photon Microscopy

James¹,

Kim²,

Hockberger³

et al. 2011

Front. Neurosci.

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Neuroblasts born in the adult subventricular zone (SVZ) migrate long distances in the rostral migratory stream (RMS) to the olfactory bulbs where they integrate into circuitry as functional interneurons. As very little was known about the dynamic parameters of SVZ neuroblast migration, we used two-photon time-lapse microscopy to analyze migration in acute slices. This involved analyzing 3D stacks of images over time and uncovered several novel aspects of SVZ migration: chains remain stable, cells can be immotile for extensive periods, morphology does not necessarily correlate with motility, neuroblasts exhibit local exploratory motility, dorsoventral migration occurs throughout the striatal SVZ, and neuroblasts turn at distinctive angles. We investigated these novel findings in the SVZ and RMS from the population to the single cell level. In this review we also discuss some technical considerations when setting up a two-photon microscope imaging system. Throughout the review we identify several unsolved questions about SVZ neuroblast migration that might be addressed with current or emerging techniques.

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