Culturing characteristics of Hanwoo myosatellite cells and C2C12 cells incubated at 37°C and 39°C for cultured meat

Oh, Sehyuk; Park, Sanghun; Park, Yunhwan; Kim, Yun-a; Park, Gyutae; Cui, Xiang-Shun; Kim, Kwansuk; Joo, Seon-Tea; Hur, Sun; Kim, Gapdon; Choi, Jung Seok

doi:10.5187/jast.2023.e10

Cited by 6 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is involved in key reactions during myogenesis, including myoblast-to-myocyte transition, myotube fusion, and myofiber maturation [41]. Furthermore, the myosin heavy chain (MYHC) is one of the representative late-stage differentiation markers whose expression level increases as muscle cell differentiation progresses [22]. MYH2 and MYH7 are known expression factors that are used to identify myofiber types IIa and I, respectively [28].…”

Section: Discussionmentioning

confidence: 99%

“…PCR was performed using SYBR Green (ELPIS-BIOTECH, Daejeon, Korea) and the primer sequences listed in Table 1. Gene amplification was as follows: 50 • C for 2 min, 95 • C for 10 min, followed by 40 cycles of 95 • C for 10 s and 20 s between 52 and 55 • C. The mRNA quantification method used the 2 −∆∆CT method [22].…”

Section: Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

See 1 more Smart Citation

Effect of Crude Polysaccharides from Ecklonia cava Hydrolysate on Cell Proliferation and Differentiation of Hanwoo Muscle Stem Cells for Cultured Meat Production

Lee,

Kim,

Kang

et al. 2024

Foods

Self Cite

View full text Add to dashboard Cite

Ecklonia cava, a brown seaweed native to the East Asian coast, is known for its unique composition, including polysaccharides, polyphenols, and phlorotannins. Fucoidan is a sulfated polysaccharide widely used as a functional ingredient in foods. This study obtained crude polysaccharides (ECC_CPS) from E. cava celluclast enzymatic hydrolysate using ethanol precipitation. ECC_CPS increased cell viability during the proliferation of Hanwoo muscle satellite cells (HMSCs). The effect of ECC_CPS on the expression of proliferation-related markers was confirmed as MYF5 and MYOD expression significantly increased, whereas PAX7 expression was maintained. The evaluation of cell migration activity has a major impact on cell proliferation and differentiation, and the cell migration index significantly increased with ECC_CPS treatment (p < 0.01). This was related to the HGF/MET pathway and FAK pathway. Treatment with ECC_CPS promoted differentiation at the cell differentiation stage, thereby increasing the expression of differentiation markers, such as MYH2, MYH7, and MYOG (p < 0.001 or p < 0.01). Therefore, our findings imply that crude polysaccharide obtained from E. cava can be an additive ingredient that enhances the proliferation and differentiation of muscle satellite cells used in the manufacture of cultured meat products.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

Effect of Crude Polysaccharides from Ecklonia cava Hydrolysate on Cell Proliferation and Differentiation of Hanwoo Muscle Stem Cells for Cultured Meat Production

Lee,

Kim,

Kang

et al. 2024

Foods

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some environmental cues like mild high temperature (39°C) and mechanical movement have also been demonstrated to enhance myogenic differentiation and hypertrophy ( Guan et al, 2022 ). Consequently, while inducing heat stress through elevated culture temperatures may not independently suffice for cell growth and differentiation, it can effectively promote growth factor-mediated cell proliferation and differentiation ( Oh et al, 2023 ).…”

Section: Manufacturing Of Cultured Meatmentioning

confidence: 99%

Industrial Research and Development on the Production Process and Quality of Cultured Meat Hold Significant Value: A Review

Kang,

Lee,

Kim

2024

Food Sci Anim Resour

View full text Add to dashboard Cite

Cultured meat has been gaining popularity as a solution to the increasing problem of food insecurity. Although research on cultured meat started later compared to other alternative meats, the industry is growing rapidly every year, with developed products evaluated as being most similar to conventional meat. Studies on cultured meat production techniques, such as culturing new animal cells and developing medium sera and scaffolds, are being conducted intensively and diversely. However, active in-depth research on the quality characteristics of cultured meat, including studies on the sensory and storage properties that directly influence consumer preferences, is still lacking. Additionally, studies on the combination or ratio of fat cells to muscle cells and on the improvement of microbiota, protein degradation, and fatty acid degradation remain to be conducted. By actively investigating these research topics, we aim to verify the quality and safety of cultured meats, ultimately improving the consumer preference for cultured meat products.

show abstract

Improved culture procedure for bovine muscle satellite cells for cultured meat

Yun,

Lee,

Lee

et al. 2023

Food Research International

View full text Add to dashboard Cite

Culturing characteristics of Hanwoo myosatellite cells and C2C12 cells incubated at 37°C and 39°C for cultured meat

Abstract: Culturing characteristics of Hanwoo myosatellite cells incubated different temperature.

Cited by 6 publications

References 43 publications

Effect of Crude Polysaccharides from Ecklonia cava Hydrolysate on Cell Proliferation and Differentiation of Hanwoo Muscle Stem Cells for Cultured Meat Production

Effect of Crude Polysaccharides from Ecklonia cava Hydrolysate on Cell Proliferation and Differentiation of Hanwoo Muscle Stem Cells for Cultured Meat Production

Industrial Research and Development on the Production Process and Quality of Cultured Meat Hold Significant Value: A Review

Improved culture procedure for bovine muscle satellite cells for cultured meat

Contact Info

Product

Resources

About