“…To avoid the loss of heat and fluid from the wound, the generation of coverage becomes necessary. Autologous cultured epidermal substitutes can be another alternative [3], but when dermal bed has been completely destroyed, this approach drives to unsatisfactory results owing to the fragility of the graft and wound contraction and scar formation [4,5].…”
Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and might contribute to the understanding of skin regeneration. The use of dermal papilla cells (DPCs) as a dermal component in a permanent composite skin with human hair follicle stem cells (HFSCs) was evaluated by studying the tissue-engineered skin architecture, stem cell persistence, hair regeneration, and graft-take in nude mice. A porcine acellular dermal matrix was seeded with HFSCs alone and with HFSCs plus human DPCs or dermal fibroblasts (DFs). In vitro, the presence of DPCs induced a more regular and multilayered stratified epidermis with more basal p63-positive cells and invaginations. The DPC-containing constructs more accurately mimicked the skin architecture by properly stratifying the differentiating HFSCs and developing a well-ordered epithelia that contributed to more closely recapitulate an artificial human skin. This acellular dermal matrix previously repopulated in vitro with HFSCs and DFs or DPCs as the dermal component was grafted in nude mice. The presence of DPCs in the composite substitute not only favored early neovascularization, good assimilation and remodeling after grafting but also contributed to the neovascular network maturation, which might reduce the inflammation process, resulting in a better healing process, with less scarring and wound contraction. Interestingly, only DPC-containing constructs showed embryonic hair bud-like structures with cells of human origin, presence of precursor epithelial cells, and expression of a hair differentiation marker. Although preliminary, these findings have demonstrated the importance of the presence of DPCs for proper skin repair.
“…To avoid the loss of heat and fluid from the wound, the generation of coverage becomes necessary. Autologous cultured epidermal substitutes can be another alternative [3], but when dermal bed has been completely destroyed, this approach drives to unsatisfactory results owing to the fragility of the graft and wound contraction and scar formation [4,5].…”
Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and might contribute to the understanding of skin regeneration. The use of dermal papilla cells (DPCs) as a dermal component in a permanent composite skin with human hair follicle stem cells (HFSCs) was evaluated by studying the tissue-engineered skin architecture, stem cell persistence, hair regeneration, and graft-take in nude mice. A porcine acellular dermal matrix was seeded with HFSCs alone and with HFSCs plus human DPCs or dermal fibroblasts (DFs). In vitro, the presence of DPCs induced a more regular and multilayered stratified epidermis with more basal p63-positive cells and invaginations. The DPC-containing constructs more accurately mimicked the skin architecture by properly stratifying the differentiating HFSCs and developing a well-ordered epithelia that contributed to more closely recapitulate an artificial human skin. This acellular dermal matrix previously repopulated in vitro with HFSCs and DFs or DPCs as the dermal component was grafted in nude mice. The presence of DPCs in the composite substitute not only favored early neovascularization, good assimilation and remodeling after grafting but also contributed to the neovascular network maturation, which might reduce the inflammation process, resulting in a better healing process, with less scarring and wound contraction. Interestingly, only DPC-containing constructs showed embryonic hair bud-like structures with cells of human origin, presence of precursor epithelial cells, and expression of a hair differentiation marker. Although preliminary, these findings have demonstrated the importance of the presence of DPCs for proper skin repair.
“…1 Numerous researchers applied the method of cultured keratinocytes clinically for the treatment of severely burned patients. 16,17 However, application of 3T3 cells for keratinocyte expansion is associated with the risk of contamination with infections and animal antigens. 18 At present, the focus of research is to discover an optimal protocol for keratinocyte culture.…”
“…Sin embargo, los pacientes que reciben las células autólogas cultivadas tiene tasas de mortalidad mucho menores que aquellos que reciben tratamientos convencionales. Al mismo tiempo, se reporta que en estos pacientes se ha reducido la necesidad de intervenciones quirúrgicas y el dolor causado por las lesiones (CotoSegura et al, 2007;Carsin et al, 2000y Wood, Kolybaba y Allen, 2006. Por otro lado, cuando las lesiones no son muy extensas ni profundas, este tipo de tratamiento sí puede representar una disminución de costos, asociados principalmente a la reducción del tiempo requerido para sanar, lo que implica menos gastos en personal médico, menos insumos y menor tiempo de internamiento.…”
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