Cultured Epithelial Autografts for Giant Congenital Nevi

Gallico, G. Gregory; O’Connor, Nick; Compton, Carolyn C.; Remensnyder, John P.; Kehinde, Olaniyi; Green, Howard

doi:10.1097/00006534-198907000-00001

Cited by 188 publications

(49 citation statements)

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“…Since the original development of a culture method for epidermal keratinocytes by Rheinwald and Green (1975), keratinocytes co-cultured with 3T3 feeder layers *Correspondence to : Teruo Okano,Institute of Advanced Biomedical Engineering and Science,Tokyo Women's Medical University,TWIns, have been used for preparing epidermal grafts in the treatment of skin defects, such as severe burns (Gallico et al, 1984), ulcers (Phillips et al, 1989) and giant congenital naevi (Gallico et al, 1989). Similar to successes in the clinical applications of epidermal keratinocytes cocultured with 3T3 feeder layers, the culture methods of Rheinwald and Green have been also used for oral epithelial cells (Muller-Glauser and Preisig, 1983).…”

Section: Introductionmentioning

confidence: 99%

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Takagi

Yamato

Murakami

et al. 2010

J Tissue Eng Regen Med

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Keratinocyte culture medium (KCM) has been used for the in vitro culture of keratinocytes and other types of epithelial cells, and the medium includes various ingredients. In this study, two modified KCMs were prepared. In the first, insulin, hydrocortisone and antibiotics that are normally included in KCM were replaced with clinically approved pharmaceutical agents, except transferrin and selenium; in the second, cholera toxin (CT) was replaced by L-isoproterenol (ISO). The modified KCMs were then compared to conventional KCM containing laboratory-grade reagents. Induced cell colony formations of canine oral mucosal epithelial cells cultured in both modified KCMs were found to be nearly equivalent to that in the control KCM, and there was no significant difference between the effect of CT and ISO. Canine oral mucosal cells proliferated to confluence in all three KCM formulations, with or without the use of 3T3 feeder layers. Cultured epithelial cells were harvested from temperature-responsive culture surfaces as an intact cell sheet, and the immunohistochemical analysis of the sheets showed that p63 and cytokeratin were expressed in the epithelial cell sheets cultured in all KCMs. Eventually, in the modified KCM formula, fetal bovine serum was replaced by autologous human serum, and the formula was found to be able to fabricate human oral mucosal epithelial cell sheets. These results indicated that the modified KCM was equally efficient as conventional KCM in the fabrication of transplantable stratified epithelial cell sheets.

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Section: Introductionmentioning

confidence: 99%

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Takagi

Yamato

Murakami

et al. 2010

J Tissue Eng Regen Med

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“…[2][3][4] These investigators reported that cultured autografts of keratinocytes had persistent problems, which included blistering, wound contracture, susceptibility to infection, and varying graft "take" rate. The problems were most likely due to the lack of a dermal component and of a mature dermoepidermal junction.…”

Section: Introduction Smentioning

confidence: 99%

Evaluation of Transplanted Tissue-Engineered Oral Mucosa Equivalents in Severe Combined Immunodeficient Mice

et al. 2003

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The aim of this study was to determine the optimal stage of development at which transplant human ex vivo-produced oral mucosa equivalents (EVPOMEs) in vivo. EVPOMEs were generated in a serum-free culture system, without the use of an irradiated xenogeneic feeder layer, by seeding human oral keratinocytes onto a human cadaveric dermal equivalent, AlloDerm. EVPOMEs were cultured for 4 days submerged and then for 7 or 14 days at an air-liquid interface to initiate stratification before transplantation into SCID mice. AlloDerm, without epithelium, was used as a control. Mice were killed on days 3, 10, and 21 posttransplantation. Epithelium of the transplanted EVPOMEs was evaluated with the differentiation marker keratin 10/13. Dermal microvessel ingrowth was determined by immunohistochemistry with a mouse vascular marker, lectin binding from Triticum vulgaris. The presence and stratification of the epithelium were correlated with revascularization of the underlying dermis. The microvessel density of AlloDerm without epithelium was less than that of EVPOMEs with an epithelial layer. Microvessel density of the dermis varied directly with the degree of epithelial stratification of the EVPOMEs. The EVPOMEs cultured at an air-liquid interface for 7 days had the optimal balance of neoangiogenesis and epithelial differentiation necessary for in vivo grafting. 163

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“…Alternate to solve all these problems are the tissue expanders [12,13]. Classical expanders and self-inflating expanders [osmotic tissue expanders] are available.…”

Section: Discussionmentioning

confidence: 99%

Giant Congenital Melonocytic Nevi of Face-Primary Excision with Reconstruction Using Self-Filling Osmotic Expanders

Venugopal

2015

Indian J Surg

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Congenital hairy nevi or giant congenital melanocytic nevi (GCMN) especially that of the face pose a great challenge to reconstructive surgeons. These unsightly lesions can be psychologically damaging to both parent and child. A variety of treatment options exist for the management of such cases. In this review article, we present two children who had a GCMN of their face managed with self-inflatable tissue expanders (osmotic tissue expanders [OTEs]) for single stage excision, and reconstruction is presented. The uses of self-inflating tissue expanders are now a regular choice for tissue cover in wide areas and in children.

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Cultured Epithelial Autografts for Giant Congenital Nevi

Cited by 188 publications

References 0 publications

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Evaluation of Transplanted Tissue-Engineered Oral Mucosa Equivalents in Severe Combined Immunodeficient Mice

Giant Congenital Melonocytic Nevi of Face-Primary Excision with Reconstruction Using Self-Filling Osmotic Expanders

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