Cultured chick sympathetic neurons: ATP-induced noradrenaline release and its blockade by nicotinic receptor antagonists

Allgaier, Clemens; Wellmann, Henning; Schobert, Angelika; Kurz, Gerhart; Kügelgen, Ivar von

doi:10.1007/bf00169186

Cited by 28 publications

(12 citation statements)

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“…In cultured chick and rat sympathetic neurons, nucleotide P2‐receptors, when activated, initiate action potential firing and release of previously stored [ 3 H]noradrenaline (Boehm, 1994 ; Allgaier et al, 1995 b ; von Kügelgen et al, 1997). In the mouse neurons, however, ATP did not change the basal outflow of tritium, i.e., did not release [ 3 H]noradrenaline.…”

Section: Discussionmentioning

confidence: 99%

Noradrenaline Release from Cultured Mouse Postganglionic Sympathetic Neurons

Trendelenburg

Cox

Gaiser

et al. 1999

Journal of Neurochemistry

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Abstract:The possible existence of ␣ 2 -autoreceptors, P2-autoreceptors, and adenosine A 1 -or A 2A -receptors was studied in cultured thoracolumbar postganglionic sympathetic neurons from mice. The cells were preincubated with [ 3 H]noradrenaline and then superfused. The selective ␣ 2 -adrenoceptor agonist UK 14,304 reduced the electrically evoked overflow of tritium. When the cultures were stimulated by trains of increasing pulse number, ranging from a single pulse to 72 pulses at 3 Hz, the concentration-inhibition curve of UK 14,304 was shifted progressively to the right and the maximal inhibition obtainable became progressively smaller. Six ␣-adrenoceptor antagonists shifted the concentration-inhibition curve of UK 14,304 in a parallel manner to the right. Neither ATP (3-300 M), adenosine (0.01-100 M), the selective A 1 -receptor agonist cyclopentyladenosine (1-1,000 nM), nor the selective A 2A -receptor agonist CGS-21680 (1-10,000 nM) changed the basal or the electrically evoked overflow of tritium. It is concluded that the cultured neurons possess presynaptic, release-inhibiting ␣ 2 -autoreceptors. As in intact tissues, the effectiveness of presynaptic ␣ 2 -adrenergic inhibition depends on the "strength" of the releasing stimulus. The pK D values of the six antagonists against UK 14,304 indicate that the autoreceptors belong to the pharmacological ␣ 2D and hence the genetic ␣ 2A/D subtype of ␣ 2 -adrenoceptor. Neither P2-autoreceptors nor receptors for adenosine, the degradation product of ATP, were detected. Key Words: Mouse sympathetic neuron cultures-Noradrenaline release -␣ 2 -Autoreceptors-P2-receptors-Adenosine receptors. J. Neurochem. 73, 1439Neurochem. 73, -1445Neurochem. 73, (1999.In the preceding communication, we have described basic properties of the release of [ 3 H]noradrenaline from cultured thoracolumbar postganglionic sympathetic neurons from mice (Trendelenburg et al., 1999). Some evidence was found that the neurons possessed ␣ 2 -autoreceptors. We have now identified and examined the ␣ 2 -autoreceptors more thoroughly. Sympathetic neurons may in addition possess autoreceptors for ATP, a cotransmitter of noradrenaline (von Kügelgen and Starke, 1991), and receptors for adenosine, the degradation product of ATP. The possible occurrence of these receptors on the cultured neurons was also investigated. Some results have been published in abstract form (Trendelenburg et al., 1998a). EXPERIMENTAL PROCEDURES Culturing and [ 3 H]noradrenaline releaseThe procedure was that of the preceding article (Trendelenburg et al., 1999). In brief, mouse thoracolumbar sympathetic neurons were cultured for 7 days on Thermanox discs 6 mm in diameter. After the culturing period, the 6-mm discs were preincubated with [ 3 H]noradrenaline, subsequently placed in superfusion chambers, and superfused with [ 3 H]noradrenalinefree medium at 25°C and a rate of 0.6 ml/min. Successive 2-min samples of the superfusate were collected from t ϭ 60 min onwards (t ϭ 0 being the start of superfusion). Seven periods of electr...

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Section: Discussionmentioning

confidence: 99%

Noradrenaline Release from Cultured Mouse Postganglionic Sympathetic Neurons

Trendelenburg

Cox

Gaiser

et al. 1999

Journal of Neurochemistry

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“…During early postnatal life, IB 4 -binding DRG neurons (that express P2X3 receptors) switch from nerve growth factor to glial cell-derived neurotrophic factor dependence [222]. P2 receptors modulated NA release from chick sympathetic neurons cultured from 12-dayold embryos [223,224]. This suggested that both a facilitatory P2 receptor and an inhibitory receptor were involved.…”

Section: Peripheral Nervous Systemmentioning

confidence: 99%

Purinergic signalling during development and ageing

Burnstock

Dale

2015

Purinergic Signalling

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Extracellular purines and pyrimidines play major roles during embryogenesis, organogenesis, postnatal development and ageing in vertebrates, including humans. Pluripotent stem cells can differentiate into three primary germ layers of the embryo but may also be involved in plasticity and repair of the adult brain. These cells express the molecular components necessary for purinergic signalling, and their developmental fates can be manipulated via this signalling pathway. Functional P1, P2Y and P2X receptor subtypes and ectonucleotidases are involved in the development of different organ systems, including heart, blood vessels, skeletal muscle, urinary bladder, central and peripheral neurons, retina, inner ear, gut, lung and vas deferens. The importance of purinergic signalling in the ageing process is suggested by changes in expression of A1 and A2 receptors in old rat brains and reduction of P2X receptor expression in ageing mouse brain. By contrast, in the periphery, increases in expression of P2X3 and P2X4 receptors are seen in bladder and pancreas.

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“…A study of P2 receptors modulating NA release from chick sympathetic neurons cultured from 12-day-old embryos suggested that two different P2 receptor subtypes were involved: a facilitatory receptor and an inhibitory receptor [147]. In a sister paper, the authors postulated that the ATP receptor involved in NA release acted via a subclass of the nicotinic cholinoceptor [148].…”

Section: Gangliamentioning

confidence: 99%

Purinergic signaling in embryonic and stem cell development

Burnstock

Ulrich

2011

Cell. Mol. Life Sci.

112

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Nucleotides are of crucial importance as carriers of energy in all organisms. However, the concept that in addition to their intracellular roles, nucleotides act as extracellular ligands specifically on receptors of the plasma membrane took longer to be accepted. Purinergic signaling exerted by purines and pyrimidines, principally ATP and adenosine, occurs throughout embryologic development in a wide variety of organisms, including amphibians, birds, and mammals. Cellular signaling, mediated by ATP, is present in development at very early stages, e.g., gastrulation of Xenopus and germ layer definition of chick embryo cells. Purinergic receptor expression and functions have been studied in the development of many organs, including the heart, eye, skeletal muscle and the nervous system. In vitro studies with stem cells revealed that purinergic receptors are involved in the processes of proliferation, differentiation, and phenotype determination of differentiated cells. Thus, nucleotides are able to induce various intracellular signaling pathways via crosstalk with other bioactive molecules acting on growth factor and neurotransmitter receptors. Since normal development is disturbed by dysfunction of purinergic signaling in animal models, further studies are needed to elucidate the functions of purinoceptor subtypes in developmental processes.

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Cultured chick sympathetic neurons: ATP-induced noradrenaline release and its blockade by nicotinic receptor antagonists

Cited by 28 publications

References 39 publications

Noradrenaline Release from Cultured Mouse Postganglionic Sympathetic Neurons

Noradrenaline Release from Cultured Mouse Postganglionic Sympathetic Neurons

Purinergic signalling during development and ageing

Purinergic signaling in embryonic and stem cell development

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