Cultured autologous keratinocytes in the treatment of large and deep burns: A retrospective study over 15 years

An evaluation of the choice of feeder cell growth arrest for the production of cultured epidermis

“…The lowered keratinocyte proliferation by the MC feeders may reflect longer waiting period for the patients with major and extensive burns. But considering the high costs of producing the cultured epidermis [3,4], the MC feeders may still be useful, particularly in the low and middle-income group of countries where the incidence and mortality due to burns is high and cost-effectiveness of burn treatment is the top agenda [29,30]. However, the final product must be tested for the traces of MC in addition to fulfilling the other regulatory requirements before evaluating the clinical utility of the thinner epidermis produced by the MC approach.…”

“…Rapid ex-vivo turnover of epidermal keratinocytes was achieved by employing the growth-arrested 3T3 feeder cells leading to large-scale production of epidermal sheets for autologous application in burns [1][2][3][4]. The choice of feeder cell growth arrest in epithelial and stem cell culture has been a subject of debate.…”

Using negative pressure wound therapy on microskin autograft wounds

“…Extensive skin defect is a great challenge and major problem in plastic and reconstructive surgery [1,2]. Autologous skin grafting has been the standard for coverage of open wounds, and there are several options for skin defects existing [3].…”

Bioprinting of Skin

“…Another promising approach is cell-based therapy to repair and regenerate skin tissue [12][13][14][15][16][17][18][19]. This method involves the use of cells to cover the wound defect and promote healing through regeneration.…”

“…This method involves the use of cells to cover the wound defect and promote healing through regeneration. To efficiently place the skin cells uniformly onto the wound, bioprinting technology has been proposed as a delivery method [12,17,[20][21][22][23]. Bioprinting of the skin seeks to deliver skin cells and components in their proper anatomical configuration to facilitate regeneration of the damaged skin while restoring function without scarring and damaging other tissues and organs of the body.…”