Cultured autologous keratinocytes in fibrin glue suspension, exclusively and combined with STS-allograft (preliminary clinical and histological report of a new technique)

Kaiser, Hans-Wilhelm; Stark, G. B.; Kopp, Jürgen; Balcerkiewicz, A.; Spilker, G.; Kreysel, H. W.

doi:10.1016/0305-4179(94)90101-5

Cited by 118 publications

(52 citation statements)

References 20 publications

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“…Fibrin glue (Beriplast) is widely used and successfully used in tissue engineering of articular cartilage (chondrocytes) as well as in tissue engineering of skin and mucosa. [51][52][53] Isogai et al 54 reported that a combination of fibrin glue and cultured periosteal cells had a feasibility of initiating site-directed formation of bone structures at heterotopic tissue site. But there were no major experiences with fibrin in bone engineering and controversy exists to whether fibrin glues act as osteogenic or osteostimulatory agents.…”

Section: Discussionmentioning

confidence: 98%

Comparative in vitro study of the proliferation and growth of human osteoblast‐like cells on various biomaterials

Itthichaisri

Wiedmann-Al-Ahmad

Huebner

et al. 2007

J Biomedical Materials Res

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In vitro studies about the growth behavior of osteoblasts onto biomaterials is a basic knowledge and a screening method for the development and application of scaffolds in vivo. In this in vitro study human osteoblast-like (HOB) cells were cultured on seven different biomaterials used in dental and craniomaxillofacial surgery, respectively. The tested biomaterials were synthetic biodegradable (MacroPore, Ethisorb, PDS, Beriplast P) and nonbiodegradable polymers (Palacos) as well as calcium phosphate cement (BoneSource) and titanium. The cell proliferation and cell colonization were analyzed by scanning electron microscopy and EZ4U-test. Statistical analysis were performed. HOB-like cells cultivated on Ethisorb showed the highest proliferation rate. The proliferation rate was statistically significant compared with Palacos, MacroPore, and BoneSource. Whereas, Beriplast, PDS, and titanium yielded lower proliferation rates. However, there was no statistically significant difference compared with Palacos, MacroPore, and BoneSource. SEM analysis showed no significant difference in individual cell features and cell colonization. But an infiltration and a growth of HOB-like cells throughout the porous structure of Ethisorb, which is formed by crossing fibers, is a striking different feature (macrotopography). This feature can explain the highest proliferation rate of Ethisorb. The results showed that HOB-like cells appear to be sensitive to substrate composition and topography. Moreover, the basis for further studies with such biomaterial/osteoblast constructs in vivo are provided. Further focusing points are developing techniques to fabricate three-dimensional porous biomaterial/cell constructs, studying the tissue reaction and the bone regeneration of such constructs compared with the use of autologous bone.

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Section: Discussionmentioning

confidence: 98%

Comparative in vitro study of the proliferation and growth of human osteoblast‐like cells on various biomaterials

Itthichaisri

Wiedmann-Al-Ahmad

Huebner

et al. 2007

J Biomedical Materials Res

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“…Ronfard et al (1991) used keratinocytes, which were cultured for a short time on fibrin gel, as a treatment for thermal burns and obtained good clinical results. Kaiser et al (1994) cultured autologous keratinocytes with fibrin and developed them into an epidermal sheet, applied it in the treatment of thermal burns, and obtained a high take rate. Stark and Kaiser (1994) improved the take rates in the treatment of burns by combining glycerolpreserved allogenic skin with autologous keratinocytes cultured on fibrin gel.…”

confidence: 99%

Fibrin Stimulates the Proliferation of Human Keratinocytes through the Autocrine Mechanism of Transforming Growth Factor-.ALPHA. and Epidermal Growth Factor Receptor

Yamamoto

Yanaga²,

Nishina³

et al. 2005

Tohoku J. Exp. Med.

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In the field of dermatology and plastic and reconstructive surgery, fibrin gel is regarded as a material that promotes wound healing. To test the hypothesis that fibrin may promote the growth of the epidermis, we examined its effects on the proliferation of cultured keratinocytes. Human keratinocytes were cultivated in fibrincoated wells, and the cell numbers and transforming growth factor (TGF)-α , secreted into the cultured medium, were measured. We also assessed the capacity of epidermal growth factor receptor (EGF-R) that is responsible for all known actions of TGF-α and epidermal growth factor. The keratinocytes increased dramatically in their number, and the TGF-α secretion and the binding capacity of EGF-R were also increased dramatically in the presence of fibrin. These findings suggest that fibrin supports the proliferation of keratinocytes in an autocrine fashion via EGF-R; namely, fibrin stimulates keratinocytes to secrete TGF-α, which in turn increases cell proliferation and EGF-R capacity. We propose that fibrin can support the wound healing process of the epidermis via the TGF-α /EGF-R pathway.fibrin; keratinocytes; TGF-α ; EGF-R; wound healing © 2005 Tohoku University Medical PressFibrin gel is commonly used in clinical applications, as it had been originally developed as a biological glue for wound closure and tissue fixation. Therefore, it has mainly been used to stop bleeding and the loss of bodily fluid. Recently in the fields of dermatology and plastic and reconstructive surgery, fibrin gel began to be regarded as a material that promotes the wound-healing process and results in a good take rate for epidermis grafts and in faster wound healing.There are several clinical reports on the use of fibrin gel as a wound healing promoter. Ronfard

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“…Another advantage of cultured keratinocyte allografts is the large surface area obtained from a relative small biopsy of healthy skin from the patient. However fragility and difficult handling of the grafts, and unpredictable take and extremely high costs are other major disadvantages [8]. Storage and preservation of viable sheets have also been a major problem.…”

Section: Epidermal Reconstruction Keratinocyte Culturesmentioning

confidence: 99%

“…Cell therapy is an emerging therapeutic strategy aimed at replacing or repairing severely damaged tissues with cultured cells. Epidermal regeneration obtained with autologous cultured keratinocytes (cultured epithelial autografts (CEAs) can be life-saving for patients suffering from massive full thickness burns [8,9]. Cultured epithelia avoid the mesh aspect obtained with a split thickness autograft as well as the discomfort of skin graft harvesting for the patient; however there is a time delay of 2-5 weeks for culture of the autologous sheets of keratinocytes.…”

Section: Epidermal Reconstruction Keratinocyte Culturesmentioning

confidence: 99%

Review Paper: Burn Coverage Technologies: Current Concepts and Future Directions

2006

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Cultured autologous keratinocytes in fibrin glue suspension, exclusively and combined with STS-allograft (preliminary clinical and histological report of a new technique)

Cited by 118 publications

References 20 publications

Comparative in vitro study of the proliferation and growth of human osteoblast‐like cells on various biomaterials

Comparative in vitro study of the proliferation and growth of human osteoblast‐like cells on various biomaterials

Fibrin Stimulates the Proliferation of Human Keratinocytes through the Autocrine Mechanism of Transforming Growth Factor-.ALPHA. and Epidermal Growth Factor Receptor

Review Paper: Burn Coverage Technologies: Current Concepts and Future Directions

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