Cultured Autologous Human Cells for Hard Tissue Regeneration: Preparation and Characterization of Mesenchymal Stem Cells from Bone Marrow

Kotobuki, Noriko; Hirose, Motohiro; Takakura, Yoshinori; Ohgushi, Hajime

doi:10.1111/j.1525-1594.2004.07320.x

Cited by 152 publications

(115 citation statements)

References 28 publications

(25 reference statements)

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“…To differentiate EBs into neuronal cells, retinoic acid (5 M) was treated for 4 days (Okada et al, 2004). Neuronal differentiation of MSCs was performed as described previously (Kotobuki et al, 2004).…”

Section: Embryonic Body Formation and Neuronal Differentiationmentioning

confidence: 99%

Dynamic changes of gangliosides expression during the differentiation of embryonic and mesenchymal stem cells into neural cells

Kwak

Yu²,

Kim³

et al. 2006

Exp Mol Med

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Stem cells are used for the investigation of developmental processes at both cellular and organism levels and offer tremendous potentials for clinical applications as an unlimited source for transplantation. Gangliosides, sialic acid-conjugated glycosphingolipids, play important regulatory roles in cell proliferation and differentiation. However, their expression patterns in stem cells and during neuronal differentiation are not known. Here, we investigated expression of gangliosides during the growth of mouse embryonic stem cells (mESCs), mesenchymal stem cells (MSCs) and differentiated neuronal cells by using high-performance thin-layer chromatography (HPTLC). Monosialoganglioside 1 (GM1) was expressed in mESCs and MSCs, while GM3 and GD3 were expressed in embryonic bodies. In the 9-day old differentiated neuronal cells from mESCs cells and MSCs, GM1 and GT1b were expressed. Results from immunostaining were consistent with those observed by HPTLC assay. These suggest that gangliosides are specifically expressed according to differentiation of mESCs and MSCs into neuronal cells and expressional difference of gangliosides may be a useful marker to identify differentiation of mESCs and MSCs into neuronal cells.

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Section: Embryonic Body Formation and Neuronal Differentiationmentioning

confidence: 99%

Dynamic changes of gangliosides expression during the differentiation of embryonic and mesenchymal stem cells into neural cells

Kwak

Yu²,

Kim³

et al. 2006

Exp Mol Med

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“…MSCs derived from her father and a healthy donor were prepared as previously described. 11 In this study, cryopreserved cells were thawed and recultured in basal medium. Cells were used at passage 2 for plasmid transfection and retroviral transduction.…”

Section: Culture Of Mscsmentioning

confidence: 99%

“…10 We have used human MSCs from the patient's bone marrow for treating various diseases centering on bone diseases since 2001. [11][12][13] MSCs and/or osteoblasts differentiated from MSCs have been transplanted into more than 80 patients.…”

Section: Introductionmentioning

confidence: 99%

Restoration of cellular function of mesenchymal stem cells from a hypophosphatasia patient

et al. 2009

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Mesenchymal stem cells (MSCs) can differentiate into multiple cell lineages and are used for regenerative treatments for a variety of diseases. However, the patient's cells cannot be used to treat genetic diseases. Allogeneic cells can serve as an alternative but long-term survival is uncertain. Our experience of allo-transplantation to a patient with hypophosphatasia, which is caused by mutations of the tissue non-specific alkaline phosphatase (TNSALP) gene resulting in low serum alkaline phosphatase (ALP) activity and skeletal deformity, did not improve these clinical characteristics. Therefore, we sought to use autologous MSCs for the treatment of hypophosphatasia. MSCs derived from the patient's bone marrow had a similar profile when compared with well-reported MSCs. However, the MSCs had extremely low ALP activity and could not produce a mineralized bone matrix even under the osteogenic culture conditions. We therefore transduced a retroviral vector with TNSALP promoter-driven TNSALP gene in the MSCs. In the culture condition, the MSCs had about 7-fold higher ALP activity than did mock-transduced MSCs, and showed mineralization as well as bone-specific markers. Furthermore, the MSCs, but not mock-transduced MSCs, newly formed bone at the frequency of 50% in nude rats. Transplantation of the TNSALP-transduced autologous MSCs might become a new therapy for hypophosphatasia.

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“…18 Recent studies have demonstrated that MSCs have the ability to aggregate at the site of tissue or organ damage and differentiate into multiple cell types of the mesenchymal lineage, 31,48,56,57 suggesting great potential in their application to gene therapy and the treatment of heart, brain, and necrotic limb disease. 1,10,33 It is the migratory potential of stem cells that allows for their use in therapeutics: Implanted MSCs are able to migrate to the injury site, suggesting that an innate response to the injured microenvironment may produce attractive signals such as cytokines and chemokines to recruit those MSCs. 26,42 The underlying mechanisms that mediate these responses, however, are still unclear in part due to the methodological limitations.…”

Section: Importance Of Stem Cell Migration In Physiology and Medicinementioning

confidence: 99%

Computational Modeling of Stem Cell Migration: A Mini Review

et al. 2014

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Abstract-Stem cell migration is crucial in many biological processes such as embryogenesis, histogenesis, and stem cell biology. It is also an important process in physiology, and in medicine, it can contribute to develop effective stem cell therapies. While there is a large body of experimental evidence that attempts to understand the biological and physiological mechanisms governing stem cell activity in the organism, computational modeling studies are scarce. Because stem cell migration is affected by biological diversity and the complexity of the cells' microenvironment, experiments are hard to conduct and corresponding measurements are sophisticated. Computational modeling is a good complementary method to help us understand this process. Here, a mini-review is presented discussing the existing efforts and the unsolved key issues in stem cell migration. In addition, existing computational models studying the migration of differentiated cells are briefly discussed in the context of how they can be applied to increase our understanding of the dynamics involved in stem cell migration: Particular emphasis is placed on how biomechanical aspects of migration are explored in these models.

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Cultured Autologous Human Cells for Hard Tissue Regeneration: Preparation and Characterization of Mesenchymal Stem Cells from Bone Marrow

Cited by 152 publications

References 28 publications

Dynamic changes of gangliosides expression during the differentiation of embryonic and mesenchymal stem cells into neural cells

Dynamic changes of gangliosides expression during the differentiation of embryonic and mesenchymal stem cells into neural cells

Restoration of cellular function of mesenchymal stem cells from a hypophosphatasia patient

Computational Modeling of Stem Cell Migration: A Mini Review

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