Cultured allogenic keratinocytes for extensive burns: A retrospective study over 15 years

Auxenfans, Céline; Shipkov, Hristo; Bach, C.; Catherine, Zulma; Lacroix, P; Bertin‐Maghit, M.; Damour, O.; Braye, F.

doi:10.1016/j.burns.2013.05.005

Cited by 41 publications

(21 citation statements)

References 30 publications

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“…Studies evaluating expansion of keratinocytes on human fibroblasts following trypsin extraction [138], and using engineered skin with keratinocytes on a fibrin matrix [139], have demonstrated improvements in wound healing. Retrospective analyses on autologous keratinocytes showed that cultured allogeneic or autologous keratinocytes may accelerate wound healing [140, 141]. Taken together, the future impact of keratinocyte-mediated cell coverage options is promising, but more research is needed [134].…”

Section: Optimization Of Burn Wound Healingmentioning

confidence: 99%

Burn wound healing and treatment: review and advancements

et al. 2015

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Burns are a prevalent and burdensome critical care problem. The priorities of specialized facilities focus on stabilizing the patient, preventing infection, and optimizing functional recovery. Research on burns has generated sustained interest over the past few decades, and several important advancements have resulted in more effective patient stabilization and decreased mortality, especially among young patients and those with burns of intermediate extent. However, for the intensivist, challenges often exist that complicate patient support and stabilization. Furthermore, burn wounds are complex and can present unique difficulties that require late intervention or life-long rehabilitation. In addition to improvements in patient stabilization and care, research in burn wound care has yielded advancements that will continue to improve functional recovery. This article reviews recent advancements in the care of burn patients with a focus on the pathophysiology and treatment of burn wounds.

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Section: Optimization Of Burn Wound Healingmentioning

confidence: 99%

Burn wound healing and treatment: review and advancements

et al. 2015

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“…Researchers have also used keratinocytes as one component in cellular constructs and reported that they contributed to improved quality of wound healing [176,177]. A retrospective study by Auxenfans et al [178] showed that cultured allogenic keratinocytes allowed fast healing of deep second degree burns in human patients. Kazemi-Darabadi et al [179] described that allogenous skin fibroblast transplantation for diabetic wounds in sheep positively affected the wound healing by increasing re-epithelialization, number of fibroblasts and blood vessels.…”

Section: Regenerative Therapies For Skin Wound Managementmentioning

confidence: 99%

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Borena

Martens

Broeckx

et al. 2015

Cell Physiol Biochem

168

112

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Mammal skin has a crucial function in several life-preserving processes such as hydration, protection against chemicals and pathogens, initialization of vitamin D synthesis, excretion and heat regulation. Severe damage of the skin may therefore be life-threatening. Skin wound repair is a multiphased, yet well-orchestrated process including the interaction of various cell types, growth factors and cytokines aiming at closure of the skin and preferably resulting in tissue repair. Regardless various therapeutic modalities targeting at enhancing wound healing, the development of novel approaches for this pathology remains a clinical challenge. The time-consuming conservative wound management is mainly restricted to wound repair rather than restitution of the tissue integrity (the so-called “restitutio ad integrum”). Therefore, there is a continued search towards more efficacious wound therapies to reduce health care burden, provide patients with long-term relief and ultimately scarless wound healing. Recent in vivo and in vitro studies on the use of skin wound regenerative therapies provide encouraging results, but more protracted studies will have to determine whether the effect of observed effects are clinically significant and whether regeneration rather than repair can be achieved. For all the aforementioned reasons, this article reviews the emerging field of regenerative skin wound healing in mammals with particular emphasis on growth factor- and stem cell-based therapies.

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“…To eliminate the possibility that i3T3 contributed to the telomerase activity measured in keratinocytes cultured with a feeder layer, we have evaluated the telomerase activity level in i3T3 fibroblasts alone. Given that human dermal fibroblasts can be utilized as an alternative to i3T3 feeder layers to support keratinocytes growth (Braye et al, ; Auxenfans et al, ), telomerase activity was also assessed in iHFL. Approximately 3% and 8% of iHFLs and i3T3, respectively, remain in the cell culture after keratinocytes were harvested for the measurement of telomerase activities at near‐confluence (Bisson et al, ).…”

Section: Resultsmentioning

confidence: 99%

Section: The Human Feeder Layer Preserves Telomerase Activity Of Cultmentioning

confidence: 99%

Contribution of Sp1 to Telomerase Expression and Activity in Skin Keratinocytes Cultured With a Feeder Layer

Bisson

Paquet

Bourget

et al. 2014

Journal Cellular Physiology

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The growth of primary keratinocytes is improved by culturing them with a feeder layer. The aim of this study was to assess whether the feeder layer increases the lifespan of cultured epithelial cells by maintaining or improving telomerase activity and expression. The addition of an irradiated fibroblast feeder layer of either human or mouse origin (i3T3) helped maintain telomerase activity as well as expression of the transcription factor Sp1 in cultured keratinocytes. In contrast, senescence occurred earlier, together with a reduction of Sp1 expression and telomerase activity, in keratinocytes cultured without a feeder layer. Telomerase activity was consistently higher in keratinocytes grown on the three different feeder layers tested relative to cells grown without them. Suppression of Sp1 expression by RNA inhibition (RNAi) reduced both telomerase expression and activity in keratinocytes and also abolished their long-term growth capacity suggesting that Sp1 is a key regulator of both telomerase gene expression and cell cycle progression of primary cultured human skin keratinocytes. The results of the present study therefore suggest that the beneficial influence of the feeder layer relies on its ability to preserve telomerase activity in cultured human keratinocytes through the maintenance of stable levels of Sp1 expression.

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Cultured allogenic keratinocytes for extensive burns: A retrospective study over 15 years

Cited by 41 publications

References 30 publications

Burn wound healing and treatment: review and advancements

Burn wound healing and treatment: review and advancements

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Contribution of Sp1 to Telomerase Expression and Activity in Skin Keratinocytes Cultured With a Feeder Layer

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