Culture versus PCR for<i>Salmonella</i>Species Identification in Some Dairy Products and Dairy Handlers with Special Concern to Its Zoonotic Importance

Gwida, Mayada; Al-Ashmawy, Maha

doi:10.1155/2014/502370

Cited by 22 publications

(22 citation statements)

References 25 publications

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“…Different studies have shown very high prevalence rate of Salmonella contamination of vegetables [8,14] and different animal products [6,21,22]. The prevalence rates from these studies compares favorably with the 20% of the 120 study samples and the 96% of the 25 pure Salmonella isolates of DNA-PCR Salmonella invA gene detectable results obtained in this current study.…”

Section: Discussionsupporting

confidence: 76%

Molecular detection of salmonella species from selected vegetables sold in a north-central Nigerian setting

Adeniyi¹,

Akindigh²,

Aniweta³

et al. 2016

Af J Clin Exp Micro

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It is vital to study and understand the genetic basis to the virulence of different Salmonella strains in other to fully grasp the facts behind the unique capabilities of these pathogenic agents to causing diseases in both humans and animals. In this study, the conventional microbiological culture methods were used to isolate pure Salmonella strains from 120 vegetable samples of five different types; which were all obtained at seven different popular markets in the Jos Metropolis of North-Central Nigeria. 25 (20.8%) pure isolates were obtained from 120 samples after initial culture and sub-cultures; with 24 (20%) of the pure isolates testing positive as being pathogenic after biochemical analysis. From the 25 pure isolates, the same 24 which tested positive for biochemical tests were also successfully amplified by PCR technique with the Salmonella invA virulence gene. The result shows that 96% of the pure isolates were positive for the Salmonella invA gene. The PCR product which was very specific is a 250bp fragment of DNA which was visualized in 1.5% agarose gel. This finding shows that virulent Salmonella strains pose a major health hazard and public health concern to the affected population. Our study shows that there is a high prevalence rate of virulent Salmonella strains in North-Central Nigeria. It is thus concluded that although both the conventional culture and biochemical methods of isolating Salmonella species are most useful for obtaining pure isolates and identifying pathogenic strains, however, the PCR technique remains the most specific and sensitive; especially when the rapid identification and detection of virulent strains of Salmonella species are of utmost importance. Mots clés : Virulence, le gèneinvA , PCR, Centre -nord du Nigeria.

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Section: Discussionsupporting

confidence: 76%

Molecular detection of salmonella species from selected vegetables sold in a north-central Nigerian setting

Adeniyi¹,

Akindigh²,

Aniweta³

et al. 2016

Af J Clin Exp Micro

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“…Apparently, the methods were not fast enough to keep up with the pace of animal food production. In the last decades, several PCR-based methods that include PCR [9], PCR in combination with hybridization [10], immuno-PCR by combining monoclonal antibody coated magnetic bead with PCR [11], PCR in combination with conventional culture and serotyping [12] and real-time PCR [13][14][15][16][17][18] for rapid detection of Salmonella have been reported. However, all of these methods require a pre-enrichment step of 6-28h followed by tedious DNA isolation and purification procedures to overcome PCR inhibitors.…”

Section: Introductionmentioning

confidence: 99%

Direct PCR – A rapid method for multiplexed detection of different serotypes of Salmonella in enriched pork meat samples

Chin

Sun

Høgberg

et al. 2017

Molecular and Cellular Probes

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Salmonellosis, an infectious disease caused by Salmonella spp., is one of the most common foodborne diseases. Isolation and identification of Salmonella by conventional bacterial culture method is time consuming. In response to the demand for rapid on line or at site detection of pathogens, in this study, we developed a multiplex Direct PCR method for rapid detection of different Salmonella serotypes directly from pork meat samples without any DNA purification steps. An inhibitor-resistant Phusion Pfu DNA polymerase was used to overcome PCR inhibition. Four pairs of primers including a pair of newly designed primers targeting Salmonella spp. at subtype level were incorporated in the multiplex Direct PCR. To maximize the efficiency of the Direct PCR, the ratio between sample and dilution buffer was optimized. The sensitivity and specificity of the multiplex Direct PCR were tested using naturally contaminated pork meat samples for detecting and subtyping of Salmonella spp. Conventional bacterial culture methods were used as reference to evaluate the performance of the multiplex Direct PCR. Relative accuracy, sensitivity and specificity of 98.8%; 97.6% and 100%, respectively, were achieved by the method. Application of the multiplex Direct PCR to detect Salmonella in pork meat at slaughter reduces the time of detection from 5 to 6 days by conventional bacterial culture and serotyping methods to 14 h (including 12 h enrichment time). Furthermore, the method poses a possibility of miniaturization and integration into a point-of-need Lab-on-a-chip system for rapid online pathogen detection.

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“…At Bahir Dar University, 2.7% of the food handlers tested positive for S. Typhi [7]. Additionally, studies performed amongst food handlers in Ethiopia in the southern region, University of Gondar, dairy workers, and Addis Abbaba University showed prevalence of 3.1%, 0.93%, 5.0%, 4.8% respectively [2] [6] [10] [11]. A 2015 study by David et al at a Nigerian university showed a prevalence of 35% amongst food handlers following a recent outbreak of typhoid fever [12].…”

Section: Introductionmentioning

confidence: 97%

“…Several thousand non-typhoidal serovars exist with the predominant strains varying from region to region but the most common serovars being Salmonella enteritidis (S. enteritidis) and Salmonella typhimurium (S. Typhimurium) which comprise approximately three-quarters of the global yearly cases of salmonellosis [2] [3]. Typhoidal Salmonella, divided into serovars Salmonella typhi (S. typhi) and Salmonella paratyphi (S. paratyphi), as the names suggest, are the cause of typhoid fever [1].…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of Highly Multi-Drug Resistant <i>Salmonella</i> Fecal Carriage Among Food Handlers in Lower Basic Schools in The Gambia

Kebbeh¹

2017

IJNFS

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Abstract:Background: Salmonella spp are among the most common food borne pathogens. Food handlers play an important role in the production of food products, in which they can contribute to the transmission of salmonellosis. The probability of food contamination depends mainly on the health status and personal hygiene of the food handlers and their choice of managing their health could give rise to contamination of food by multi drug resistant bacteria. Multi Drug Resistance is of global concern and poses a public health threat in combating diseases. In the developing countries there is paucity on the prevalence of salmonella carriage among food handlers. This study aims to investigate the prevalence of salmonella and to determine their antimicrobial resistance pattern. Method: A total of 500 stool samples from different food handlers were collected and analyzed with completed questionnaires. For standard isolation and identification of salmonella isolates; stool samples were enriched in buffered peptone water, standard culture and biochemical tests were used. Antimicrobial susceptibility Test (AST) was carried out using Clinical Laboratory and Standard Institute (CLSI-2015) protocol disc diffusion method. The data were analyzed using SPSS version 16 and Microsoft excel version 2010 to determine the risk factors. Results: Of 500 participants with the mean age of 38.15, 497(99.4%) were all females. Most of the participants wash their hands under running water and 271(54.2%) were certified on food handling. Among the risk factors, consuming medicines/antibiotics from street vendors showed statistical significant of salmonella carriage with (P=0.011). It was found that 13(2.6%) were salmonella carriers. AST performed on the 13 isolates show that; 13(100%), 12(92.3%), 7(53.8%), 9(69.2%) and 9(69.2%) were resistant to Ampicillin, Erythromycin, and Tetracycline, ceftriaxone and cefotaxime respectively. And 13(100%), 12(92.3%) and 7(53.8) were sensitive to Imipenem, chloramphenicol and gentamycin respectively. Conclusion: This study had found multidrug resistant salmonella isolates carriers amongst food handlers who could serve as potential reservoirs for the transmission of these infections in the communities. Thus, it is crucial to implement regular screening of food handlers and health education on food safety.

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Culture versus PCR forSalmonellaSpecies Identification in Some Dairy Products and Dairy Handlers with Special Concern to Its Zoonotic Importance

Cited by 22 publications

References 25 publications

Molecular detection of salmonella species from selected vegetables sold in a north-central Nigerian setting

Molecular detection of salmonella species from selected vegetables sold in a north-central Nigerian setting

Direct PCR – A rapid method for multiplexed detection of different serotypes of Salmonella in enriched pork meat samples

Prevalence of Highly Multi-Drug Resistant <i>Salmonella</i> Fecal Carriage Among Food Handlers in Lower Basic Schools in The Gambia

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Culture versus PCR forSalmonellaSpecies Identification in Some Dairy Products and Dairy Handlers with Special Concern to Its Zoonotic Importance

Cited by 22 publications

References 25 publications

Molecular detection of salmonella species from selected vegetables sold in a north-central Nigerian setting

Molecular detection of salmonella species from selected vegetables sold in a north-central Nigerian setting

Direct PCR – A rapid method for multiplexed detection of different serotypes of Salmonella in enriched pork meat samples

Prevalence of Highly Multi-Drug Resistant &lt;i&gt;Salmonella&lt;/i&gt; Fecal Carriage Among Food Handlers in Lower Basic Schools in The Gambia

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Prevalence of Highly Multi-Drug Resistant <i>Salmonella</i> Fecal Carriage Among Food Handlers in Lower Basic Schools in The Gambia