Culture systems

Rocha, A.M.; Smith, Gary D.

doi:10.1017/cbo9781139059053.017

Cited by 4 publications

(1 citation statement)

References 240 publications

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“…With that said, visions and realization of fully integrated biomimetic mammalian [1] gamete manipulation; [2] insemination; [3] fertilization assessment; [4] dynamic fluid embryo culture with controlled and developmentally timed gradual media additions, subtractions, and change are in development. Integration of these bioinspired steps in a practical and userfriendly manner is key, and necessary, in generating the future's ''IVF-Laboratory on a Chip'' or ''IVF-Laboratory in a Box'' (147,(165)(166)(167). These next-generation laboratory practices will more closely recapitulate the natural environment of gamete biology, preimplantation embryo development, and assessment, while automating the process and removing individual variability and subjectivity.…”

Section: Embryo Culturementioning

confidence: 99%

Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential

Swain¹,

Carrell

Cobo

et al. 2016

Fertility and Sterility

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With increased use of comprehensive chromosome screening (CCS), the question remains as to why some practices do not experience the same high levels of clinical success after implementation of the approach. Indeed, the debate surrounding the efficacy and usefulness of blastocyst biopsy and CCS continues. Importantly, several variables impact the success of an assisted reproductive technology cycle. Transfer of a euploid embryo is but one factor in an intricate system that requires numerous steps to occur successfully. Certainly, the culture environment and the manipulations of the embryo during its time in the laboratory can impact its reproductive potential. Environmental stressors ranging from culture media to culture conditions and even culture platform can impact biochemical, metabolic, and epigenetic patterns that can affect the developing cell independent of chromosome number. Furthermore, accompanying procedures, such as biopsy and vitrification, are complex and, when performed improperly, can negatively impact embryo quality. These are areas that likely still carry room for improvement within the IVF laboratory.

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