Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells

Matsuda, Yuko; Cho, Otomi; Sugita, Takashi; Ogishima, Daiki; Takeda, Satoru

doi:10.1007/s11046-018-0259-4

Cited by 42 publications

(39 citation statements)

References 31 publications

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“…can reduce the number of hyphae formed by C. albicans (Vilela et al 2015;Ribeiro et al 2017) by releasing antimicrobial compounds, which create an adverse microenvironment for pathogens (Mastromarino et al 2002;do Carmo et al 2016). Although some molecules as biosurfactants, hydrogen peroxide, lactic acid, acetic acid and bacteriocins, are already described, further studies involving the acquisition and characterization of new molecules are needed as well as elucidation of their inhibitory activities against C. albicans, particularly in terms of biofilm formation, filamentation capacity and infection potential (James et al 2016;Liang et al 2016;Eryilmaz et al 2018;Matsuda et al 2018).…”

Section: Biofilms and Hyphae Formationmentioning

confidence: 99%

“…Most of these genes either enable adhesion and invasion of the host cell or modify the pathogenic form to avoid host defence mechanisms, in addition to having the ability to form biofilms on biotic and abiotic surfaces (Mayer et al 2013). To elucidate the molecular mechanisms involved in the antifungal effects of probiotic bacteria, especially Lactobacillus sp., several in vitro studies have focused on analysis of the expression of the virulence genes of C. albicans (Kohler et al 2012;James et al 2016;Ribeiro et al 2017;Wang et al 2017;de Barros et al 2018;Matsuda et al 2018;Rossoni et al 2018). Kohler et al (2012) investigated the molecular mechanisms of C. albicans growth inhibition by the probiotic strains Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 using genome-wide expression analyses.…”

Section: Interference With Virulence Genesmentioning

confidence: 99%

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Action mechanisms of probiotics onCandidaspp. and candidiasis prevention: an update

et al. 2019

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Due to the high incidence of fungal infections caused by Candida species and their increasing resistance to antimicrobial treatments, alternative therapies such as probiotics have been studied. It has been show that several species of the genus Lactobacillus have anti-Candida activity, probably by direct inhibition, through competition for adhesion sites or production of secondary metabolites, and by indirect inhibition, through stimulation of the immune system of their host. However, the mechanisms of inhibition of these probiotics on Candida species have not yet been fully elucidated since this effect is related to more than one inhibition pathway. In the literature, several in vitro and in vivo studies have been developed seeking to elucidate the probiotics mechanisms of action. These studies have been focused on C. albicans inhibition assays, including analysis of antimicrobial activity, adherence capacity, biofilms formation, filamentation and interference on virulence genes, as well as assays of experimental candidiasis in invertebrate and vertebrate models. In this context, the purpose of this review was to gather different studies focused on the action mechanism of probiotic strains on Candida sp. and to discuss their impact on the candidiasis prevention.

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Section: Biofilms and Hyphae Formationmentioning

confidence: 99%

Section: Interference With Virulence Genesmentioning

confidence: 99%

Action mechanisms of probiotics onCandidaspp. and candidiasis prevention: an update

et al. 2019

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“…L. crispatus and L. gasseri are commensal bacteria found in both human GI tract (Walter, 2008) and lower female urogenital tract. Their implication on women's health have been explored in the recent years (Stapleton et al, 2011;Matsuda et al, 2018;Phukan et al, 2018;Takada et al, 2018). Preliminary clinical trials have shown that intro-vaginal probiotic treatment with L. crispatus (intravaginal suppository probiotic Lactin-V; Osel) could significantly lower the reoccurrence rate of urinary tract infections in premenopausal women (Stapleton et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…L. gasseri American Type Culture Collection (ATCC) 9857 has been found to significantly inhibit the adhesion of Trichomonas vaginalis to human vaginal ectocervical cells (Phukan et al, 2018). Supernatant of L. crispatus JCM 1185 and L. gasseri JCM 1131 significantly inhibited the colonization of Candida albicans by reducing the expression of biofilm formation-related genes (Matsuda et al, 2018). It is recognized that bacteria residing in different environments could develop niche-specific adaptation, providing competitive advantages for colonizing each niche (Oh et al, 2010;Kim et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Lactobacillus gasseri and Lactobacillus crispatus Isolated From Human Urogenital and Gastrointestinal Tracts

et al. 2020

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Lactobacillus crispatus and Lactobacillus gasseri are two of the main Lactobacillus species found in the healthy vaginal microbiome and have also previously been identified and isolated from the human gastrointestinal (GI) tract. These two ecological niches are fundamentally different, notably with regards to the epithelial cell type, nutrient availability, environmental conditions, pH, and microbiome composition. Given the dramatic differences between these two environments, we characterized strains within the same Lactobacillus species isolated from either the vaginal or intestinal tract to assess whether they are phenotypically and genetically different. We compared the genomes of the Lactobacillus strains selected in this study for genetic features of interest, and performed a series of comparative phenotypic assays including small intestinal juice and acid resistance, carbohydrate fermentation profiles, lactic acid production, and host interaction with intestinal Caco-2 and vaginal VK2 cell lines. We also developed a simulated vaginal fluid (SVF) to study bacterial growth in a proxy vaginal environment and conducted differential transcriptomic analysis between SVF and standard laboratory MRS medium. Overall, our results show that although strain-specific variation is observed, some phenotypic differences seem associated with the isolation source. We encourage future probiotic formulation to include isolation source and take into consideration genetic and phenotypic features for use at various body sites.

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“…A recent study suggested that probiotic supernatants have antipathogenic properties [4], which implies that probiotics may inhibit bio lm formation through cell-cell communication. However, there has been little progress in this eld to date.…”

Section: Introductionmentioning

confidence: 99%

Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherich coli biofilm formation in coculture

Song

Lou

et al. 2020

Preprint

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Background: Escherich coli (E.coli) is the principal pathogen that causes biofilm formation; the latter is associated with infectious diseases and antibiotic resistance. In our previous work, we demonstrated that probiotic microcapsules have superior biofilm inhibition capacity compared to probiotic sterile culture supernatant. Herein, the mechanism of the inhibition effects was investigated using label-free quantitative proteomics analysis. Results:The proteomic analysis characterized a total of 1655 proteins in E.coli K12MG1655 and 1431 proteins in Lactobacillus rhamnosus GG (LGG). Among them, after coculture treatment, there were 262 and differentially expressed proteins that were specific for E.coli and 291 for LGG. The differentially expressed proteins after coculture were related to cellular metabolism, the stress response, transcription, and the cell membrane. In addition, we identified five strain-specific genes in E.coli and LGG, respectively, which were consistent with the proteomics results. Conclusions:These findings indicate that LGG microcapsules may inhibit E.coli biofilm inhibition by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions. BackgroundBiofilms are complex bacterial community structures that can attach to a surface. They are connected to this surface via extracellular polymeric substances (EPS) which form a matrix composed primarily of polysaccharides, proteins, and DNA; this encapsulates the bacteria [1]. Biofilms not only cause economic losses, but also present a public health hazard. This is because the bacteria present within biofilms are much more resistant to antibiotics, disinfectants [2], and the host immune system effectors [3]. Therefore, it is critical to uncover effective non-toxic, or less toxic, antifungal agents with novel modes of action.A recent study suggested that probiotic supernatants have antipathogenic properties [4], which implies that probiotics may inhibit biofilm formation through cell-cell communication. However, there

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Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells

Cited by 42 publications

References 31 publications

Action mechanisms of probiotics onCandidaspp. and candidiasis prevention: an update

Action mechanisms of probiotics onCandidaspp. and candidiasis prevention: an update

Comparative Analysis of Lactobacillus gasseri and Lactobacillus crispatus Isolated From Human Urogenital and Gastrointestinal Tracts

Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherich coli biofilm formation in coculture

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