Culture Supernatants of
            <i>Campylobacter jejuni</i>
            Induce a Secretory Response in Jejunal Segments of Adult Rats

Fernández, Heriberto; Neto, Ulysses Fagundes; Fernandes, Fernando; Pedra, Marly de Almeida; Trabulsi, Luíz Rachid

doi:10.1128/iai.40.1.429-431.1983

Cited by 31 publications

(14 citation statements)

References 12 publications

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“…The results of the present study, indicating that C. jejuni infection induces net Na + and CIsecretion and decreases sugar absorption, are in consonance with the in vivo studies by Fernandez et ah [4] in rat small intestine.…”

Section: Discussionsupporting

confidence: 93%

“…A significantly lower o-glucose and o-galactose absorption has been observed in young mice when Campylobacter cultures were given orally [3]. A net sodium secretory flux and decreased glucose absorption has also been shown in vivo in rat jejunal segments perfused with culture supernantants of C. jejuni [4]. The pathophysiological mechanism(s) by which C. jejuni enterotoxin induces fluid and electrolyte loss from the intestinal epithelium of experimental animals and humans is least understood.…”

Section: Introductionmentioning

confidence: 99%

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Impairment of Na+,K+-ATPase activity following enterotoxigenic Campylobacter jejuni infection: changes in Na+, Cl− and ? transport in vitro, in rat ileum

Kanwar¹

1994

FEMS Microbiology Letters

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Unidirectional fluxes of Na+, Cl- and 3-O-methyl-D-glucose (3-MG) were measured in vitro across Campylobacter jejuni live culture-infected and control rat ileal short-circuited tissues by the Ussing Chamber technique. Net secretion of Na+ and enhanced secretion of Cl- ions was observed in the infected animals (P < 0.001, n = 6) as compared to the net absorption of Na+ and marginal secretion of Cl- ions in the control animals. There was a significant decrease in the mucosal-to-serosal fluxes of 3-MG in C. jejuni-infected rat ileum. The specific Na+,K(+)-ATPase activity when measured biochemically in the membrane-rich fraction of enterocytes was found to be significantly lower (58%) in the infected group as compared to the control group (P < 0.001). Our results therefore suggest that infection with an enterotoxigenic C. jejuni inhibits the Na+,K(+)-ATPase activity in rat enterocytes. The impairment of Na+,K(+)-ATPase activity thus appears to induce a secondary change in Na+,Cl- and 3-MG transport in vitro in rat ileum.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Impairment of Na+,K+-ATPase activity following enterotoxigenic Campylobacter jejuni infection: changes in Na+, Cl− and ? transport in vitro, in rat ileum

Kanwar¹

1994

FEMS Microbiology Letters

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“…In contrast, Ruiz-Palacios and his colleagues in Mexico, using special culture conditions, have recently shown that many C. jejuni clinical isolates from children produce a heat-labile enterotoxin that raises intracellular cyclic AMP levels, causes cytotonic changes in CHO cells, and induces fluid secretion in ligated rat ileal loops; the secretory activity of this toxin is neutralized by antiserum to cholera toxin (CT) (41). Culture supernatants of these toxigenic strains have also been shown to cause intraluminal secretion of fluid and electrolytes when perfused in vivo through rat jejunal segments (16). * Corresponding author.…”

mentioning

confidence: 99%

Properties of crude Campylobacter jejuni heat-labile enterotoxin

Klipstein¹,

Engert²

1984

Infect Immun

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The amount of crude Campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure Escherichia coli heat-labile toxin (LT) in the Chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used GM ganglioside or antisera to LT or both. Maximum C. jejuni enterotoxin production was achieved by growth at 42°C for 24 b under agitation in supplemented GC medium. Adding polymyxin separately to either the broth supernatant or the cells enhanced the recovery of toxin; the yield from cell lysates was much lower. The quantity of C. jejuni enterotoxin produced by clinical isolates obtained locally or provided from Mexico varied widely, over a spectrum from none to large amounts; quantitative values for the amount of C. jejuni enterotoxin determined by the Chinese hamster ovary and enzyme-linked immunosorbent assays correlated with the degree of secretory potency of this material in ligated rat ileal loops. The cytotonic activity of C. jejuni enterotoxin in Chinese hamster ovary cells was abolished by heating at 96°C for 10 min and by preincubation either with GM ganglioside or with LT or cholera toxin antisera. The secretory activity of C. jejuni enterotoxin in ligated rat ileal loops was passively neutralized by antiserum to LT, and immunizing rats with either LT or its B subunit significantly (P < 0.001) reduced fluid response to active challenge with C. jejuni enterotoxin in ligated ileal loops. These observations indicate that strains of C. jejuni vary in their capacity to elaborate a heat-labile enterotoxin that has close immunological homology with LT and cholera toxin.

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“…Both methods display high sensitivity and specificity. In vivo assays to detect enterotoxin activity include the rabbit or rat ileal loop test (RILT) (32,84), or the removable intestinal tie adult rabbit diarrhea (RITARD) model (109).…”

Section: Enterotoxinsmentioning

confidence: 99%

Toxin production by Campylobacter spp

Wassenaar

1997

Clin Microbiol Rev

141

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Of all the virulence factors that were proposed for Campylobacter jejuni and related species to cause disease in humans, the discovery of toxin production was the most promising but led to a rather confusing and even disappointing stream of data. The discussion of whether proteinaceous exotoxins are relevant in disease remains open. One important reason for this lack of consensus is the anecdotal nature of the literature reports. To provide a basis for an unbiased opinion, this review compiles all described exotoxins, compares their reported properties, and provides a summary of animal model studies and clinical data. The toxins are divided into enterotoxins and cytotoxins and are sorted according to their biochemical properties. Since many Campylobacter toxins have been compared with toxins of other species, some key examples of the latter are also discussed. Future directions of toxin research that appear promising are defined.

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Culture Supernatants of Campylobacter jejuni Induce a Secretory Response in Jejunal Segments of Adult Rats

Abstract: Culture supernatants offour Campylobacterjejuni strains induced a net sodium secretory flux (plasma-lumen) and an impaired glucose transport in perfused jejunal segments of adult rats in vivo.

Cited by 31 publications

References 12 publications

Impairment of Na+,K+-ATPase activity following enterotoxigenic Campylobacter jejuni infection: changes in Na+, Cl− and ? transport in vitro, in rat ileum

Impairment of Na+,K+-ATPase activity following enterotoxigenic Campylobacter jejuni infection: changes in Na+, Cl− and ? transport in vitro, in rat ileum

Properties of crude Campylobacter jejuni heat-labile enterotoxin

Toxin production by Campylobacter spp

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