Culture site dependence on pearl size realization in Pinctada margaritifera in relation to recipient oyster growth and mantle graft biomineralization gene expression using the same donor phenotype

Abstract

International audienceSize is the most important and valuable quality of the cultured black-lip pearl, Pinctada margaritifera. As this pearl aquaculture is carried out at numerous grow-out sites, this study analyzes the environmental influence on pearl size parameters (nacre weight and thickness) in relation to the recipient oyster biometric parameters (shell thickness, height, width, and oyster weight) at harvest time. Toward this end, an experimental graft was designed by using a homogeneous donor oyster phenotype. The recipient oysters were randomly and equally transferred and reared in five commercial and contrasting grow-out locations. Overall inter-site comparisons revealed that the cultured pearl size (N = 2168) and the biometric parameters of the recipient oysters were highest for sites with warmer temperatures with low seasonal variation in comparison to the southern latitude sites. These results were supported by positive correlations between pearl nacre thickness and recipient oyster shell thickness, height, and width. In parallel, the biomineralization potential of the mantle graft was screened through four genes encoding aragonite (Pif 177, MSI60) and calcite (shematrin 9, aspein). As the gene expression levels were the same among all the donor oysters, this finding demonstrates that: 1) the pearl sac that originated from the mantle graft was not isolated from environmental variations during the culture period and 2) the phenotypic expressions of the two biomineralizing tissues in the recipient oyster were consistent (shell and pearl). In the near future, this knowledge will be helpful at the production sites of genetically selected donor oyster lines for growth produced in hatchery systems. Culture site dependence on pearl size realization in Pinctada margaritifera in relation to recipient oyster growth and mantle graft biomineralization gene expression using the same donor phenotype. Available from: https://www.researchgate.net/publication/299384948_Culture_site_dependence_on_pearl_size_realization_in_Pinctada_margaritifera_in_relation_to_recipient_oyster_growth_and_mantle_graft_biomineralization_gene_expression_using_the_same_donor_phenotype [accessed Oct 18 2017]

Influence of preoperative food and temperature conditions on pearl biogenesis in Pinctada margaritifera

“…Pearl production is also a complex process that involves genetic contributions from two oysters (donor and recipient), which may be affected by the environment. Although the donor oyster is primarily responsible for the expression pattern of biomineralization genes in the pearl sac at both genomic (Arnaud-Haond et al, 2007) and transcriptomic levels (McGinty et al, 2012), the recipient oyster is strongly suspected to regulate pearl sac metabolism (Le Pabic et al, 2016) The grafter skills also influence pearl biomineralization and quality (Ky et al, 2015b. Recently, significant correlations have been demonstrated between pearl…”

Freshwater nucleated pearl quality is influenced by host mussel growth traits in Hyriopsis cumingii

“…Additionally, even though the nucleus had no direct contact with the host mussel from inside the pearl sac, nacre thickness was directly correlated with the amount of nacre biomineralization. Higher biomineralization capabilities of the host mussels we selected may have contributed to an increased nacre deposition, which has been previously observed in Pinctada margaritifera (Blay, Sham‐Koua, Vonau, Tetumu, Cabral & Ky ; Le Pabic, Parrad, Sham, Nakasai, Saulnier, Devaux & Ky ). In Pinctada fucata martensii , a positive correlation between cultured pearl traits (diameter, weight and nacre thickness) and growth traits of the host oyster (weight and shell height) has also been detected (Wada & Komaru ).…”

Potential combinations of mabé, keshi and cultured pearl production from colourful hatchery-produced Pinctada margaritifera

“…This same pattern was already noticed in a previous study on P. margaritifera, where shell weight was correlated with pearl size (nacre weight and thickness). Indeed, several positive correlations were observed between cultured pearl size and shell biometric parameters, with cultured pearl nacre thickness showing a significant positive correlation with recipient oyster shell thickness, height, and width, and nacre weight correlated with shell thickness (Le Pabic et al, 2016).…”