Culture results from wound biopsy versus wound swab: does it matter for the assessment of wound infection?

Haalboom, Marieke; Blokhuis-Arkes, Miriam H.E.; Beuk, R.J.; Meerwaldt, R.; Klont, Rob; Schijffelen, M.J.; Bowler, Philip G; Burnet, Michael; Sigl, Eva; Palen, Job van der

doi:10.1016/j.cmi.2018.08.012

Cited by 35 publications

(33 citation statements)

References 21 publications

(23 reference statements)

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“…The diagnosis of CW infection currently relies on a combination of clinical judgment and microbiological cultivation of one of the potential specimens: swabs, which are non-invasive and more frequently used or wound tissue, obtained by a more demanding and invasive biopsy or curettage (16). Recent guidelines for CW biofilms specify tissue biopsies as a gold standard for microbiological diagnostics due to the sampling of both surface and deeper tissue (4).…”

Section: Introductionmentioning

confidence: 99%

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Comparison Between Cultivation and Sequencing Based Approaches for Microbiota Analysis in Swabs and Biopsies of Chronic Wounds

et al. 2021

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Chronic wounds are a prominent health concern affecting 0.2% of individuals in the Western population. Microbial colonization and the consequent infection contribute significantly to the healing process. We have compared two methods, cultivation and 16S amplicon sequencing (16S-AS), for the characterization of bacterial populations in both swabs and biopsy tissues obtained from 45 chronic wounds. Using cultivation approach, we detected a total of 39 bacterial species, on average 2.89 per sample (SD = 1.93), compared to 5.9 (SD = 7.1) operational taxonomic units per sample obtained with 16S-AS. The concordance in detected bacteria between swab and biopsy specimens obtained from the same CWs was greater when using cultivation (58.4%) as compared to 16S-AS (25%). In the entire group of 45 biopsy samples concordance in detected bacterial genera between 16S-AS and cultivation-based approach was 36.4% and in swab samples 28.7%. Sequencing proved advantageous in comparison to the cultivation mainly in case of highly diverse microbial communities, where we could additionally detect numerous obligate and facultative anaerobic bacteria from genera Anaerococcus, Finegoldia, Porphyromonas, Morganella, and Providencia. Comparing swabs and biopsy tissues we concluded, that neither sampling method shows significant advantage over the other regardless of the method used (16S-AS or cultivation). In this study, chronic wound microbiota could be distributed into three groups based on the bacterial community diversity. The chronic wound surface area was positively correlated with bacterial diversity in swab specimens but not in biopsy tissues. Larger chronic wound surface area was also associated with the presence of Pseudomonas in both biopsy and swab specimens. The presence of Corynebacterium species at the initial visit was the microbial marker most predictive of the unfavorable clinical outcome after one-year follow-up visit.

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Section: Introductionmentioning

confidence: 99%

“…However, only few studies up to date systematically evaluated the differences between the two approaches (19)(20)(21)(22). Previous studies specifically performed on CWs used either sequencing-based methods (23) or cultivationbased methods (16,24,25) to compare between the swab and biopsy specimens.…”

Section: Introductionmentioning

confidence: 99%

Comparison Between Cultivation and Sequencing Based Approaches for Microbiota Analysis in Swabs and Biopsies of Chronic Wounds

et al. 2021

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“…10,12 These limitations result in delayed treatment, potentially leading to a worse prognosis for the patient. 4 Recent technological advancements have led to the development of assays that utilise genomic markers to rapidly identify pathogenic bacteria (ELISA, PCR, DNA microarrays and optical and electrochemical biosensors). [5][6][7][8][9][10][11][12] However, as with conventional methods, most of these techniques are expensive and require extensive sample manipulation by trained specialists.…”

Section: Introductionmentioning

confidence: 99%

TCF-ALP: a fluorescent probe for the selective detection of Staphylococcus bacteria and application in “smart” wound dressings

et al. 2021

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“…The diagnosis of CW infection currently relies on a combination of clinical judgement and microbiological cultivation of different specimens: swabs, which are non-invasive and more frequently used, or wound tissue, obtained by a more demanding and invasive biopsy or curettage (14). Recent guidelines for CW biofilms specify tissue biopsies as a gold standard for microbiological diagnostics due to the sampling of both surface and deeper tissue (4).…”

Section: Introductionmentioning

confidence: 99%

“…However, only few studies up to date systematically evaluated the differences between the two approaches (17)(18)(19). Previous studies specifically performed on CWs used either sequencing-based methods (20) or cultivation-based methods (14,21,22) to compare between the swab and biopsy specimens.…”

Section: Introductionmentioning

confidence: 99%

Comparison between cultivation and sequencing based approaches for microbiota analysis in swabs and biopsies of chronic wounds

Mahnič

Breznik

Ihan

et al. 2020

Preprint

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Chronic wounds are a prominent health concern affecting 0.2% of individuals in the Western population. Microbial colonization and the consequent infection contribute significantly to the healing process of chronic wounds. We have compared cultivation and 16S amplicon sequencing (16S-AS) for the characterization of bacterial populations in swabs and biopsy tissues obtained from 45 chronic wounds and analysed metadata for wound-specific and clinical-outcome-associated correlations with bacterial community structure.Using cultivation approach, we detected a total of 39 bacterial species, on average 2.89 per sample (SD=1.93). Comparison of cultivation results between swabs and biopsy samples showed no significant advantage of one sampling method over the other. 16S-AS was advantageous in comparison to the cultivation approach in case of highly diverse communities, where we could additionally detect numerous obligate and facultative anaerobic bacteria from genera Anaerococcus, Finegoldia, Porphyromonas, Morganella and Providencia. Based on the community diversity, chronic wound microbiota could be distributed into three groups, however, no correlation between groups and clinical outcome was observed. Clinically estimated presence of biofilm and a larger surface area at the initial visit were most significantly associated with unfavourable clinical outcomes after one-year follow-up visit. Corynebacterium was the single most predictive bacterial genus associated with unfavourable clinical outcomes in our study.

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Culture results from wound biopsy versus wound swab: does it matter for the assessment of wound infection?

Cited by 35 publications

References 21 publications

Comparison Between Cultivation and Sequencing Based Approaches for Microbiota Analysis in Swabs and Biopsies of Chronic Wounds

Comparison Between Cultivation and Sequencing Based Approaches for Microbiota Analysis in Swabs and Biopsies of Chronic Wounds

TCF-ALP: a fluorescent probe for the selective detection of Staphylococcus bacteria and application in “smart” wound dressings

Comparison between cultivation and sequencing based approaches for microbiota analysis in swabs and biopsies of chronic wounds

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