Culture phenotypes and molecular characterization of Mycobacterium avium subsp. paratuberculosis isolates from small ruminants

Dimareli-Malli, Z.; Mazaraki, K.; Stevenson, Karen; Tsakos, P.; Ζdragas, Αntonios; Giantzi, Virginia; Petridou, Evanthia; Heron, Ian; Vafeas, G.

doi:10.1016/j.rvsc.2013.03.010

Cited by 14 publications

(17 citation statements)

References 26 publications

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“…The control and eradication of this disease is difficult partly due to the lack of a reliable, sensitive, and specific diagnosis test. Paratuberculosis in small ruminants is widely distributed in several European countries, such as France (Mercier et al 2010), Greece (Dimareli-Malli et al 2013) as well as in many Asian countries (Kumthekar et al 2013, Lee et al 2006. On the American continent caprine paratuberculois has been reported in USA (Pithua and Kollias 2012), Canada (Debien et al 2013) and Chile (Kruze et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

et al. 2015

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Isolation and characterization of RESUMENLa paratuberculosis, causada por Mycobacterium avium subsp. paratuberculosis (MAP), es una infección crónica e incurable del tracto intestinal de los animales. El estudio se realizó en 2012, usando muestras procedentes de 491 cabras negras coreanas, menores de 2 años, procedentes de un rebaño caprino de la región Sur de Corea. Los animales se evaluaron para detectar presencia de paratuberculosis. Los métodos utilizados para investigar las infecciones por MAP fueron ensayo por inmunoabsorción ligado a enzimas y cultivos microbiológicos. De las 491 cabras de 100 rebaños seleccionados, 4 (0,8%) y 3 (0,6%) dieron resultados positivos para MAP en la prueba de ELISA y el estudio microbiológico de coprocultivo, respectivamente. Las colonias aisladas fueron confirmadas como MAP por IS900-PCR y su tipificación se realizó por medio del ensayo de IS1311 PCR-REA. Los resultados revelaron una baja tasa de prevalencia de infecciones por MAP de cepas de "tipo bovino" y "tipo bisonte".Palabras claves: cabra, Mycobacterium avium subsp. paratuberculosis, cultivo. SUMMARYParatuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic incurable infection of intestinal tract of animals. The study took place in 2012, on 491 Korean black goats, older than 2 years, from goat herds located in Southern region, Korea. The animals were sampled and tested for paratuberculosis. Enzyme-linked immunosorbent assay and microbiological culture were used to investigate MAP infection. Among the 491 goats from 100 herds screened, 4 (0.8%) and 3 (0.6%) goats were defined positive for MAP by ELISA and faecal culture, respectively. Isolated colonies were confirmed as MAP by IS900-PCR, and genotyped by IS1311 PCR-REA assays. The results revealed MAP infection at a low prevalence rate with "cattle type" and "bison type" genotypes.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

et al. 2015

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“…Paratuberculosis was first recognized in Greek goats in 1975 (Leontides et al, 1975). Today, the majority of Greek goat flocks are endemically infected with MAP (Ikonomopoulos et al, 2007;Dimareli-Malli et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Flock-level factors associated with the risk of Mycobacterium avium subsp. paratuberculosis (MAP) infection in Greek dairy goat flocks

Angelidou

Kostoulas

Leontides

2014

Preventive Veterinary Medicine

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“…Furthermore, MAP from subclinical animals due to intermittent disposal and low number of bacteria in feces and milk (Whipple et al, 1991). Dimareli-Malli et al (2013) studied the phenotypic and genotypic characteristics of MAP isolated from small ruminants and concluded that, for the primary isolation, only M7H11 medium should be used, or even a combination of M7H11 and HEYM, which promotes the growth of the main types of MAP and reduces the period of incubation at 37 • C. In this study, no animals with clinical signs of the disease were observed and only HEYM medium was used for cultivation. Such data could have contributed to the reduced number of isolates obtained and the absence of strain S (sheep).…”

Section: Discussionmentioning

confidence: 99%

Molecular typing of Mycobacterium avium subsp. paratuberculosis (MAP) isolated from dairy goats in Brazil

Souza

Lima

Braga

et al. 2016

Small Ruminant Research

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a b s t r a c tIn the last decade there was a significant increase dairy goat participation in the world and Brazilian agricultural scenario. Minas Gerais state is the third largest producer of goat milk in Brazil. Paratuberculosis is a chronic intestinal disease that mostly affects ruminants and can become a public health problem due to the possible association with Crohn's disease. It is transmitted by ingesting Mycobacterium avium subsp. paratuberculosis (MAP) contaminated food or water. In recent years, with the development of molecular techniques, the disease has been identified with greater precision and more quickly. MAP strains (S-Sheep, Cattle-C and B-Bison) have also been typed. The detection of a circulating MAP strain in a herd is important to develop epidemiological studies and thus establish better strategies to control paratuberculosis. This study aimed to identify and type MAP in dairy goat farms in the Zona da Mata, the main producing region of Minas Gerais state, Brazil. Feces and milk samples of 467 animals were collected, processed, inoculated in Herrold's Egg Yolk Agar (HEYM) and submitted to PCR and REA techniques. Eleven (2.36%) animals were positive for the presence of MAP in four properties and the isolates were characterized as type C strain. It was concluded that MAP is present in dairy goats from properties in Zona da Mata, MG and that strain type C circulates in the area. This is the first report of MAP typing isolated from dairy goat in Brazil.

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Culture phenotypes and molecular characterization of Mycobacterium avium subsp. paratuberculosis isolates from small ruminants

Cited by 14 publications

References 26 publications

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

Flock-level factors associated with the risk of Mycobacterium avium subsp. paratuberculosis (MAP) infection in Greek dairy goat flocks

Molecular typing of Mycobacterium avium subsp. paratuberculosis (MAP) isolated from dairy goats in Brazil

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