Culture of Stentor coeruleus on Colpidium campylum*

Terra, Noël de

doi:10.1111/j.1550-7408.1966.tb01947.x

Cited by 23 publications

(7 citation statements)

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“…Stentor coeruleus cells were obtained commercially (Carolina Biological Supply, Burlington, NC) but subsequently maintained in culture within the lab by growing in the dark at 20 °C in Modified Stentor Medium (MSM), 0.75 mM Na 2 CO 3 , 0.15 mM KHCO 3 , 0.15 mM NaNO 3 , 0.15 mM KH 2 PO 4 , 0.15 mM MgSO 4 , 0.5 mM CaCl 2 , and 1.47 mM NaCl modified from the original recipes described by Tartar [4] and De Terra [27] . This medium provides no nutrients and must be supplemented with living prey.…”

Section: Methodsmentioning

confidence: 99%

The Kinase Regulator Mob1 Acts as a Patterning Protein for Stentor Morphogenesis

et al. 2014

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Section: Methodsmentioning

confidence: 99%

The Kinase Regulator Mob1 Acts as a Patterning Protein for Stentor Morphogenesis

et al. 2014

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“…Tartar and W. Balamuth . The cultures were maintained in sterile pond water or modified Peter's solution (11) containing a heterogeneous population of microorganisms fed on powdered milk and wheat grains as recommended by Tartar (53) .…”

Section: Stentor Culturesmentioning

confidence: 99%

THE CONTRACTILE PROCESS IN THE CILIATE, STENTOR COERULEUS

Huang

Pitelka

1973

The Journal of Cell Biology

155

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The structural basis for the function of microtubules and filaments in cell body contractility in the ciliate Stentor coeruleus was investigated . Cells in the extended state were obtained for ultrastructural analysis by treatment before fixation with a solution containing 10 mM EGTA, 50-80 mM Tris, 3 MM MgSO4 , 7 .5 mM NH 4C1, 10 mM phosphate buffer (pH 7 .1) . The response of Stentor to changes in the divalent cation concentrations in this solution suggests that Ca+ 2 and Mg+2 are physiologically important in the regulation of ciliate contractility . The generation of motive force for changes in cell length in Stentor resides in two distinct longitudinal cortical fiber systems, the km fibers and myonemes . Cyclic changes in cell length are associated with (a) the relative sliding of parallel, overlapping microtubule ribbons in the km fibers, and (b) a distinct alteration in the structure of the contractile filaments constituting the myonemes . The microtubule and filament systems are distinguished functionally as antagonistic contractile elements . The development of motive force for cell extension is accomplished by active microtubule-to-microtubule sliding generated by specific intertubule bridges . Evidence is presented which suggests that active shortening of contractile filaments, reflected in a reversible structural transformation of dense 4-nm filaments to tubular 10-12-nm filaments, provides the basis for rapid cell contraction . 04 INTRODUCTION

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“…The macronucleus undergoes a series of striking morphological changes during amitotic division. At stage 5, the nuclear nodes begin to coalesce until the nucleus is a compact mass in the center of the cell (stage 6 Chromosome-like bodies in the macronuclei of ciliates have been described. 8 9 17 18, 22 In a radiolarian, Aulacantha scolyrnantha, Grell9 observed long chains of Feulgen-positive rods and postulated that the chromosomes of each genome are strung together end to end to form compound chromosomes ("sammelchromosomen").…”

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MACRONUCLEAR DNA SYNTHESIS IN Stentor : REGULATION BY A CYTOPLASMIC INITIATOR

Terra¹

1967

Proc. Natl. Acad. Sci. U.S.A.

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The large ciliate Stentor coeruleus lends itself well to microsurgical procedures. By combining these techniques with autoradiography after exposure of cells to thymidine-H3, it is possible to study mechanisms regulating the occurrence of macronuclear DNA synthesis during the cell growth cycle. Transfer of nuclei between cells which are synthesizing DNA and cells which are not can indicate whether DNA synthesis is determined by the continuous presence or absence of a cytoplasmic factor. Similar experiments involving cell grafting could characterize such a factor as an initiator or an inhibitor. This paper describes these experiments and their results.Previous work on Stentor has suggested that mechanisms regulating the occurrence of several major events in the cell growth cycle are located in the cytoplasm.3-5The present results will be discussed in terms of these earlier findings and also in relation to current knowledge about regulation of nuclear DNA synthesis.Stentor coeruleus is a large heterotrich ciliate; individual organisms often reach a length of 1 mm when fully extended. Figure 1A shows the main morphological features of S. coeruleus, including the chain macronucleus, the anteriorly placed oral apparatus, and the rows of blue-green pigment granules running longitudinally between the kineties.Throughout interphase, the macronucleus exists as a chain of nodes spiralling almost the entire length of the organism and situated directly beneath the ectoplasm. At 20'C, amitotic division takes approximately eight hours. Tartar has assigned numbers to the stages of amitotic division.25 There are eight stage numbers, each covering a successive one-hour interval so that stage 1 designates the first hour of division while stage 8 refers to the eighth hour, during which cytokinesis occurs. The macronucleus undergoes a series of striking morphological changes during amitotic division. At stage 5, the nuclear nodes begin to coalesce until the nucleus is a compact mass in the center of the cell (stage 6 ; Fig. 1B

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Culture of Stentor coeruleus on Colpidium campylum*

Abstract: SYNOPSIS. A method is described for culturing Stentor coeruleus in agar‐lined petri dishes using the smaller ciliate Colpidium campylum as food organism.

Cited by 23 publications

References 6 publications

The Kinase Regulator Mob1 Acts as a Patterning Protein for Stentor Morphogenesis

The Kinase Regulator Mob1 Acts as a Patterning Protein for Stentor Morphogenesis

THE CONTRACTILE PROCESS IN THE CILIATE, STENTOR COERULEUS

MACRONUCLEAR DNA SYNTHESIS IN Stentor : REGULATION BY A CYTOPLASMIC INITIATOR

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