Culture of Roundtail Chub, Gila robusta robusta (Cyprinidae), through the Larval Period

Muth, Robert T.; Haynes, Charles M.; Carlson, Clarence A.

doi:10.2307/3670673

Cited by 4 publications

(5 citation statements)

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“…Life history knowledge of all Gila is lacking due to the widespread and rapid population declines that these species experienced prior to scientific description (Minckley and Deacon 1968;Minckley and Douglas 1991). However, both life span and age at maturity are known to vary considerably among these species (Vanicek 1967;Muth et al 1985;Johnson and Belk 1999). For example, both Bonytail and Humpback Chub G. cypha are historically co-occurring, long-lived fishes that can live for up to 40 years.…”

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confidence: 99%

Rapid Molecular Determination of Sex for Western North American Chub (Gila spp.)

Mussmann

Saltzgiver

Delomas

et al. 2021

N American J Fish Manag

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Gathering sex data is frequently a complicated matter for fish species that lack sexually dimorphic traits. Secondary sexual characteristics are cryptic or nonexistent in many minnows (Cyprinidae) during early life stages or outside of the breeding season, which may require lethal methods for determining sex. This complicates many conservation actions and population genetic studies, including (1) the development of hatchery broodstocks for threatened and endangered species, (2) sex ratio determination in wild populations, (3) the influence of sex ratio on the effective population size estimates, and (4) limitations on the implementation of genetic tagging and parentage assignment in wild populations. Here, we remedy this issue for four western North American chub species (Gila spp.) through restriction-site-associated DNA sequencing of Bonytail G. elegans, which identified a single locus indicating an XY sex determination system and no candidate loci consistent with a WZ system. This locus cross-amplified in three additional Gila species (Chihuahua Chub G. nigrescens, Virgin Chub G. seminuda, and White River Chub G. jordani), although species-specific differences necessitated the development of three novel primer/probe sets. Real-time PCR with hydrolysis probe assays was used to genotype sex-linked single-nucleotide polymorphisms for each species. The measurement of endpoint fluorescence in a real-time PCR thermal cycler allowed for rapid and accurate sex genotyping of these species, with accuracy >97% relative to phenotypic sex identification when three PCR replicates were employed. These assays provided high precision, with unanimous results across >87% of the replicates and a majority consensus genotype for 98.6% of the samples. The locus identified herein was conserved across all of the Gila species that we tested (n = 6) and other western North American cyprinids; however, sex-linked polymorphisms were found only in the four species that are named above. Consequently, this locus has promise for the identification and development of sex-linked assays for other Gila and closely related cyprinids.

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confidence: 99%

Rapid Molecular Determination of Sex for Western North American Chub (Gila spp.)

Mussmann

Saltzgiver

Delomas

et al. 2021

N American J Fish Manag

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“…Roundtail Chub, a closely related but larger species, had a greater mean fertilized egg diameter and length at hatch (Muth et al 1985) than Gila Chub. A formal description of Gila Chub larvae was not undertaken as part of our study but given the consistency with which Gila Chub will spawn in the laboratory and the proven ability to rear young to the juvenile stage, specimens needed for larval developmental studies are attainable.…”

Section: Discussionmentioning

confidence: 99%

“…Hamman (1982aHamman ( , 1982bHamman ( , 1985 reported on the spawning and reproductive biology of Humpback Chub G. cypha and Bonytail Chub G. elegans in captivity. Muth et al (1985) did the same for Roundtail Chub G. robusta. Research on spawning/culture techniques and requirements for Headwater Chub G.nigra and Roundtail Chub has been done since our study (S. A. Bonar and Erica Sontz, U.S.…”

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confidence: 99%

Spawning and Hatching of Endangered Gila Chub in Captivity

Schultz

Bonar

2016

N American J Aquac

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Information on reproductive characteristics of the endangered Gila Chub Gila intermedia is largely limited and qualitative, and culture techniques and requirements are virtually unknown. Here we provide the first published data on spawning and selected reproductive and developmental characteristics of Gila Chub. Fish were brought to the laboratory in March 2003 from Sabino Creek, Arizona (12.3°C). Fish were then warmed slowly and spawned at 14.9°C, 10 d after collection. Following this initial spawning, Gila Chub spawned consistently in the laboratory without hormonal, chemical, photoperiod, temperature, or substrate manipulation during all times of the year. Spawns were noted at temperatures ranging from about 15°C to 26°C; however, we noted that Gila Chub spawned less frequently at temperatures above 24°C. Multiple spawning attempts per year per individual are probable. There was a strong, inverse relationship between time to hatch and incubation temperature. The hatch rate of eggs was high (mean = 99.43%), and larval Gila Chub accepted a variety of natural and formulated diets at first feeding. The future of Gila Chub may someday depend in part on hatchery propagation to provide specimens for restocking formerly occupied habitats and establishing refuge populations. Information from our study can aid future efforts to successfully spawn and rear Gila Chub and related species.

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“…One hundred and twenty juvenile roundtail chub (Gila robusta) were obtained from captive reared stocks at the Bubbling Ponds Hatchery, Arizona. All chubs in the genus Gila are morphologically very similar (Douglas et al 1989), especially as juveniles (Muth et al 1985), and they are closely related systematically (Douglas and Douglas 2007) and ecologically (Kaeding et al 1990). Because it is difficult to obtain permits to sacrifice humpback chub, roundtail chub were used as a surrogate for humpback chub in this study.…”

Section: Methodsmentioning

confidence: 99%

Salinity of the Little Colorado River in Grand Canyon Confers Anti-Parasitic Properties on a Native Fish

Ward

2012

Western North American Naturalist

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Culture of Roundtail Chub, Gila robusta robusta (Cyprinidae), through the Larval Period

Cited by 4 publications

References 0 publications

Rapid Molecular Determination of Sex for Western North American Chub (Gila spp.)

Rapid Molecular Determination of Sex for Western North American Chub (Gila spp.)

Spawning and Hatching of Endangered Gila Chub in Captivity

Salinity of the Little Colorado River in Grand Canyon Confers Anti-Parasitic Properties on a Native Fish

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