Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn's disease

Naser, Saleh A.; Ghobrial, George; Romero, Claudia; Valentine, John F.

doi:10.1016/s0140-6736(04)17058-x

Cited by 545 publications

(459 citation statements)

References 29 publications

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“…Individuals with CD in the ileum also had a lower bacterial diversity in the mucosal lining of the intestine, compared to those with disease localized in the colon (Willing et al, 2009a). Several studies have implicated E. coli (Darfeuille-Michaud et al, 2004;Baumgart et al, 2007;Willing et al, 2009a) or Mycobacterium paratuberculosis (Naser et al, 2004) as potential virulent species that may have a specific role in the pathogenesis of CD, but these microbes remain to be confirmed.…”

Section: Impact Of Diseasementioning

confidence: 99%

The Gut Microbiota: Ecology and Function

Willing

Jansson

2014

The Fecal Bacteria

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Section: Impact Of Diseasementioning

confidence: 99%

The Gut Microbiota: Ecology and Function

Willing

Jansson

2014

The Fecal Bacteria

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“…A human case of systemic MAP infection has been reported (Richter et al, 2002), and some scientists have associated MAP with cases of Crohn's disease, but a causal link between MAP infection and Crohn's disease has not been proven (Naser et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium Avium Subspecies Paratuberculosis and Mycobacterium Avium Subsp. Avium Infections in a Tule Elk (Cervus Elaphus Nannodes) Herd

Crawford

Ziccardi

Gonzales

et al. 2006

Journal of Wildlife Diseases

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ABSTRACT:Between 2 August and 22 September 2000, 37 hunter-killed tule elk (Cervus elaphus nannodes) were evaluated at the Grizzly Island Wildlife Area, California, USA, for evidence of paratuberculosis. Elk were examined post-mortem, and tissue and fecal samples were submitted for radiometric mycobacterial culture. Acid-fast isolates were identified by a multiplex polymerase chain reaction (PCR) that discriminates among members of the Mycobacterium avium complex (MAC). Histopathologic evaluations were completed, and animals were tested for antibodies using a Johne's enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion. In addition, 104 fecal samples from tule elk remaining in the herd were collected from the ground and submitted for radiometric mycobacterial culture. No gross lesions were detected in any of the hunter-killed animals. Mycobacterium avium subsp. paratuberculosis (MAP) was cultured once from ileocecal tissue of one adult elk and was determined to be a strain (A18) found commonly in infected cattle. One or more isolates of Mycobacterium avium subsp. avium (MAA) were isolated from tissues of five additional adult elk. Gastrointestinal tract and lymph node tissues from 17 of the 37 elk (46%) examined had histopathologic lesions commonly seen with mycobacterial infection; however, acid-fast bacteria were not observed. All MAC infections were detected from adult elk (P 5 0.023). In adult elk, a statistically significant association was found between MAA infection and ELISA sample-to-positive ratio (S/P)$0.25 (P50.021); four of five MAA culturepositive elk tested positive by ELISA. Sensitivity and specificity of ELISA S/P$0.25 for detection of MAA in adult elk were 50% and 93%, respectively. No significant associations were found between MAC infection and sex or histopathologic lesions. Bacteriologic culture confirmed infection with MAP and MAA in this asymptomatic tule elk herd. The Johne's ELISA was useful in signaling mycobacterial infection on a population basis but could not discriminate between MAA and MAP antibodies. The multiplex PCR was useful in discriminating among the closely related species belonging to MAC.

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“…The products were resolved by electrophoresis in a 2% agarose gel for 50 minutes at 150 V. A band of the expected 389 bp size matching the positive control was considered as a positive result. The target band from 6 positive samples was cut out, purified with a GFX PCR DNA and Gel Band purification kit, e submitted to a nested PCR, 13 repurified, and sent to a commercial subcontractor f for sequencing to verify the specificity of the reaction. The overall and age-group ELISA and PCR results were compared in a 2 ϫ 2 table by using Fisher exact test (FET).…”

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Comparison of Blood Polymerase Chain Reaction and Enzyme-Linked Immunosorbent Assay for Detection of Mycobacterium Avium Subsp. Paratuberculosis Infection in Cattle and Sheep

et al. 2005

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Abstract.A study was carried out to compare the performance of enzyme-linked immunosorbent assay (ELISA) and blood polymerase chain reaction (PCR) for diagnosis of paratuberculosis in cattle and sheep. For cattle, a set of 278 samples from 1 paratuberculosis-affected Friesian farm was used; it included 80 ELISApositive samples and 198 ELISA-negative samples from an age-matched group. Ninety-four samples were from heifers and 184 were from 2-5-year-old cows. The overall analysis showed a clear association (Fisher exact test [FET] P ϭ 0.0049) but a weak negative agreement (45.3%, kappa ϭ Ϫ0.1665 Ϯ 0.0994) between the 2 tests. It reflected a moderate agreement among heifers (87.7%, kappa ϭ 0.4471 Ϯ 0.2435) and a moderate disagreement among cows (62.7%, kappa ϭ Ϫ0.3670 Ϯ 0.1057). For sheep, 496 blood samples from 53 Latxa dairy flocks were used; 180 of the blood samples were from dam/offspring pairs. The overall association between the 2 tests on ovine samples was strong (FET, P ϭ 0.0005), whereas the agreement was low (kappa ϭ 0.1622 Ϯ 0.1188). There was slightly better agreement for ewes (kappa ϭ 0.2135 Ϯ 0.1992) than for lambs (kappa ϭ 0.1193 Ϯ 0.1301). There was also a highly unlikely proportion of dam/offspring positive results (FET, P Ͻ 0.0001, kappa ϭ 0.6269 Ϯ 0.1854). Four of 6 lambs that were necropsied 1 year after testing had paratuberculosis microscopic lesions in the ileocecal valve (3 lambs) or a PCR-positive result (4 lambs). These results suggest that blood PCR testing might be a potentially useful new approach in paratuberculosis diagnosis, especially in young animals.

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Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn's disease

Cited by 545 publications

References 29 publications

The Gut Microbiota: Ecology and Function

The Gut Microbiota: Ecology and Function

Mycobacterium Avium Subspecies Paratuberculosis and Mycobacterium Avium Subsp. Avium Infections in a Tule Elk (Cervus Elaphus Nannodes) Herd

Comparison of Blood Polymerase Chain Reaction and Enzyme-Linked Immunosorbent Assay for Detection of Mycobacterium Avium Subsp. Paratuberculosis Infection in Cattle and Sheep

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