Culture of inner cell masses from in vitro-derived bovine blastocysts

Anderson, G. B.; Behboodi, E.; Pacheco, Thaisy

doi:10.1016/0093-691x(92)90256-q

Cited by 5 publications

(4 citation statements)

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“…EFG and insulin are reported to propagate the growth of ICM or embryo cells in different species (N agarajan and A nderson 1982; H arvey and K aye 1990, 1991; S paventi et al 1990; S trojek et al 1990; G oldman et al 1991; S trojek ‐B aunack et al 1991; A nderson et al 1992). While insulin was used in all the cultures performed in the present study, the EGF influence was investigated in only a small group of sheep blastocysts.…”

Section: Discussionmentioning

confidence: 99%

“…In farm animals, including cattle, sheep, goats, and pigs, several attempts to obtain ES‐cell lines have been published (H andyside et al 1987; W are and F irst 1988; M énézo et al 1989; S chellander et al 1989; F léchon et al 1990; G alli et al 1990, 1992; H assan ‐H auser et al 1990; L i and T rounson 1990; N otarianni et al 1990, 1991; P iedrahita et al 1990a,b; S trojek et al 1990; H ochereau‐de R eviers and P erreau 1991, 1992; M einecke ‐T illmann et al 1991; S trojek ‐B aunack et al 1991; A nderson et al 1992; G alli 1992; M einecke ‐T illmann and M einecke 1992a,b; M oor et al 1992; S aito et al 1992; P relle et al 1993; S tice et al 1994; S trelchenko and S tice 1994; W ells et al 1995). However, in none of these studies was it proven that the established cell lines were totipotent.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of ES‐like cell lines from ovine and caprine preimplantation embryos

Meinecke-Tillmann

Meinecke

1996

J Animal Breeding Genetics

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Embryo collection and isolation of cell linesSheep and goat embryos were collected after gestagen synchronization (Chrono-gest@, Intervet), FSH-superovulation and intrauterine insemination (sheep) or mating (goats) on day 7-8 or day 10-11 of the oestrus cycle. In some instances embryos were collected as morulae, too. Flushing was performed with prewarmed mod. Dulbecco's phosphate buffered solution supplemented with 2% FCS and containing antibiotics. After collection the embryos were washed several times in culture medium and then either brought into culture intact, or the inner cell masses (embryonic discs) were isolated microsurgically, washed and then incubated.Four to eight days after the initiation of culture colonies of cells with ES-like morphology 0931-2668/96/1304-0413 $1 1.50/0 U.S.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of ES‐like cell lines from ovine and caprine preimplantation embryos

Meinecke-Tillmann

Meinecke

1996

J Animal Breeding Genetics

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“…As feeder layer is one of the key factors influencing initial ICM cultures, many studies about effects of feeder layer have been done. On the other hand, Anderson et al (1992) showed that hatched bovine blastocysts attached to bovine oviductal epithelial cell monolayers failed to proliferate. It was suggested that a feeder cell type similar to the species of the embryo might be more ideal than of heterologous species (Bongso et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…It was suggested that a feeder cell type similar to the species of the embryo might be more ideal than of heterologous species (Bongso et al, 1994). On the other hand, Anderson et al (1992) showed that hatched bovine blastocysts attached to bovine oviductal epithelial cell monolayers failed to proliferate. In the culture of porcine primordial germ cells, the number of AP positive colonies cultured on porcine embryonic fibroblast was significantly lower than on the other feeder cells (Lee & Piedrahita, 2000).…”

Section: Discussionmentioning

confidence: 99%

Isolation and initial culture of porcine inner cell masses derived fromin vitro-produced blastocysts

Hs¹,

Son

S³

et al. 2007

Zygote

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The present study was conducted to isolate and culture inner cell mass (ICM) primarily derived from in vitro-produced blastocysts and to develop the culture conditions for the ICM cells. In Experiment 1, immunosurgically isolated ICMs of blastocysts derived from in vitro fertilization (IVF), somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) were seeded onto STO cells. Primary colonies from each isolated ICM were formed with a ratio of 28.9, 30.0 and 4.9%, respectively. In Experiment 2, blastocysts collected from IVF were directly seeded onto a feeder layer with or without zona pellucida (ZP), or were subjected to ICM isolation by immunosurgery. Primary colonies were formed in 36.8% of isolated ICMs and 19.4% in intact blastocysts without ZP. In Experiment 3, ICMs from IVF blastocysts were seeded onto STO cells, mouse embryonic fibroblast (MEF) or porcine uterine epithelial cells (PUEC). On STO and MEF cells, 34.5 and 22.2% of primary colonies were formed, respectively. However, no primary colony was formed on the PUEC or in feeder-free condition. In Experiment 4, ICMs from IVF blastocysts were cultured in DMEM + Ham's F10 (D/H medium), DMEM + NCSU-23 (D/N medium) or DMEM alone. When D/H medium or D/N medium was used, 21.7 or 44.4% of primary colony were formed, respectively, while no primary colony was formed in DMEM alone. These cells showed alkaline phosphatase activity and could be maintained for up to five passages. In suspension culture, cells formed embryoid bodies. These results demonstrate that porcine ICM could be isolated and cultured primarily from in vitro-produced blastocysts with a suitable culture system.

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„Im Wettlauf gegen die Geschwindigkeit“

Anderson¹

1998

Zirkuläre Positionen 2

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Culture of inner cell masses from in vitro-derived bovine blastocysts

Cited by 5 publications

References 0 publications

Isolation of ES‐like cell lines from ovine and caprine preimplantation embryos

Isolation of ES‐like cell lines from ovine and caprine preimplantation embryos

Isolation and initial culture of porcine inner cell masses derived fromin vitro-produced blastocysts

„Im Wettlauf gegen die Geschwindigkeit“

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