Culture of immobilized insect cells

Insect cells as factories for biomanufacturing

“…Most insect cell lines are adapted to growth in suspension, but nonadherent Sf-9 and High-Five insect cells can be also immobilized with good results (Archambault et al, 1994;Ikonomou et al, 1999;Wu and Goosen, 1996).…”

“…The use of microcarriers (porous or nonporous) in suspension in different bioreactor types (classical stirred-tank or airlift) is a good way to increase the available growth area for anchorage-dependent cells per unit of available bioreactor volume (Griffiths and Looby, 1991, Kong et al, 1999, Looby and Griffiths, 1990, Reuveny, 1990, Rundstadler et al, 1990. There are few reports on the use of microcarriers for lepidopteran cell lines, perhaps because they are essentially attachment-independent (Archambault et al, 1994). Chung et al (1993) cultivated Tn cells immobilized on collagen-coated microcarriers in a split-flow airlift bioreactor, while a comparison of immobilization of High-Five and Sf-9 cells on several types of microcarriers was made by Ikonomou et al (2002).…”

“…and its physical-chemical properties (chemical composition of the inner core and of the surface, surface charge, porosity, tortuosity, etc.) must respond to considerations of cultivation and production (seeding efficiency, immobilization efficiency, growth rate, volumetric and specific production of the immobilized cells and non-adhesion of r-protein to the support) (Archambault et al, 1994;Rundstadler et al, 1990;Wu and Goosen, 1996).…”