Culture of human parathyroid cells for transplantation

Tsuji, Kazuhiko; Fuchinoue, Shohei; Kai, Kotaro; Kawase, Tateo; Kitajima, Kumiko; Sawada, Tokihiko; Nakajima, Ichiro; Agishi, T

doi:10.1016/s0041-1345(99)00529-1

Cited by 8 publications

(5 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The transplantation methods currently available include tissue transplantation, vascularized thyroidparathyroid transplantation, and cell infusion transplantation. Compared with the first 2 transplantation methods, in vitro cultured parathyroid cells can reduce the immunogenicity of the graft, downregulate the expression of major histocompatibility complex-I (MHC-I) and MHC-II class antigens, control the number of transplanted cells, and regulate PTH content (21)(22)(23). Due to the characteristics of high survival rate, low immunogenicity, and repeated usage after cryopreservation, cell transplantation has developed rapidly in recent years.…”

Section: Discussionmentioning

confidence: 99%

Promotion of allogeneic parathyroid cell transplantation in rats with hypoparathyroidism

Niu¹,

huang²,

Gao³

et al. 2021

Gland Surg

View full text Add to dashboard Cite

Background: This study sought to establish a rat model of hypoparathyroidism by removing the rat parathyroid glands, and compare the effects of different transplantation sites and transplantation methods using a primary culture of parathyroid cells in vitro on the hormone secretion of the model rats.Methods: Male Sprague Dawley (SD) rats were selected for in vivo parathyroid gland removal, and rats with abnormal postoperative water intake, weight gain, parathyroid hormone (PTH), and blood calcium ion concentration were selected as transplant recipients and divided into the model group, brachioradialis muscle cell transplantation group, gelatin sponge group, and subcutaneous transplantation group. The parathyroid tissue was removed and the primary cell culture was performed in vitro using homozygous SD rats as graft donors. When the parathyroid cells were able to secrete PTH, transplantation was performed, and the postoperative recovery of the PTH function of the rats with different transplantation sites and methods were observed.Results: A recipient model with low PTH was successfully established, and parathyroid progenitor cells with obvious PTH secretion were obtained. Better secretion was observed in the brachioradialis cell group compare with other groups. Conclusions:The in vitro primary cell culture of the donor parathyroid cells combined with cell transplantation significantly improved the physiological function of the hypoparathyroid rats, and could potentially replace traditional clinical brachioradialis muscle tissue transplantation.

show abstract

Section: Discussionmentioning

confidence: 99%

Promotion of allogeneic parathyroid cell transplantation in rats with hypoparathyroidism

Niu¹,

huang²,

Gao³

et al. 2021

Gland Surg

View full text Add to dashboard Cite

show abstract

“…La hipocalcemia severa después de una paratiroidectomía por hiperparatiroidismo es poco común, con una hipoparatiroidismo permanente, se ha intentado realizar el alotrasplante en pacientes en los que es la única opción de tratamiento, para evitar la ingesta de grandes dosis de vitamina D y calcio, lo que incrementa el riesgo de complicaciones a largo plazo, como son: parestesia, calcinosis multiorgánica y fallo renal 6,7 . El alotrasplante de paratiroides es la terapia potencial para el hipoparatiroidismo permanente 7 con resultados variables y limitados, a pesar de haber sido reproducido en animales de experimentación y en humanos 7---9 .…”

Section: Discussionunclassified

“…Las células paratiroideas expresan débilmente antígenos del HLA clase I, el cual no es responsable de la reacción de rechazo, por lo que este tipo de tejido ocasiona una baja incidencia de rechazo 6 .…”

Section: Antecedentesunclassified

Alotrasplante de paratiroides: seguimiento a 2 años

Hermosillo-Sandoval

Leonher-Ruezga

Jiménez-Gómez

et al. 2015

Cirugía y Cirujanos

View full text Add to dashboard Cite

show abstract

“…Cells isolated from adenomatous parathyroid tissue are superior to healthy and secondary hyperplastic cells both in quantity and proliferation rate. However, because adenoma is a benign tumor, there are some concerns about its possible behavior in recipients (5,6).The potential for functional or histopathologic transformation of adenoma cells is not yet known (5)(6)(7). In this study, we aimed to pre-evaluate the usability of this tissue with xenotransplantation from human to rabbit because it has potential to be quite valuable as a cell source.…”

Section: Introductionmentioning

confidence: 99%

Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroid adenomas to normocalcemic rabbits

Ayşan

Düzköylü²,

Can

et al. 2017

Turk J Surg

View full text Add to dashboard Cite

Objective: Parathyroid allotransplantation is a new method for the treatment of permanent hypoparathyrodism. Adenoma cells are not used for transplantation because of the potential for functional or histopathologic transformation. In this study, we transplanted human adenomatous parathyroid cells to rabbits. Material and Methods:Parathyroid adenoma tissue taken from a male patient was cryopreserved and transplanted into seven New Zealand white rabbits (mean weight, 3700±220 g; mean age, 4.5 months) under immunosuppression. The levels of parathormone, calcium and phosphorus were measured before and after transplantation, and the parathyroid cells were observed histopathologically. Results:Mean parathyroid hormone level was 0.5 pg/dL before transplantation and 6.6 pg/dL after transplantation (p<0.05). Preoperative mean calciumlevel was 14.1 mg/dL, and mean phosporus level was 3.5 mg/dL before transplantation while these values were 14.4 mg/dL and 3.3mg/dL, respectively, after transplantation (p>0.05). Morphologic transformation was not observed in parathyroid cells after transplantation. Conclusion:In short-term observation, adenomatous parathyroid cells can function without malignant transformation. In the future, the preliminary methodology in this study may serve as a safe alternative for allotransplantation into patients with permanent hypoparathyroidism.Keywords: Hypoparathyroidism, xenotransplantation, parathyroid adenoma ABSTRACT Turk J Surg 2017; 33: 91-95 DOI: 10.5152/turkjsurg.2017.3427 Cite this paper as:Ayşan E, Düzköylü Y, Can İ, Büyükpınarbaşılı N. Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroid adenomas to normocalcemic rabbits. Turk J Surg 2017; 33(2): 91-95 Original InvestigationProcedure for Parathyroid Tissue Donor Patient: A 49-yearold male patient was admitted to the outpatient clinic with malignant hypercalcemia (blood calcium level >11 mg/dL). Ultrasound evaluation of the neck revealed a 3 cm diameter mass inferior to the left lobe of the thyroid tissue. Parathyroid scintigraphy (Tc 99m MIBI) indicated that the mass was a parathyroid adenoma. A small Kocher neck incision was performed, and the mass was excised and then divided into two parts. One part was used for histopathologic evaluation and the other part was cryopreserved according to thetechnique described below for xenotransplantation. The mass was reported as parathyroid adenoma inthe histopathologic evaluation. Cryopreservation and Cell Preparation:Tissue was pooled and cryopreserved in three main steps: cell isolation, cell counting (total number of cells, rate of viability) and finally storing in a liquid nitrogen tank. Tissue samples were immediately placed in ice-chilled RPMI 1640 media after removal, and the cells were isolated. The entire protocol was carried out in sterile conditions in a sterile hood. All solutions and instruments were sterilized. The tissues were gently placed in a steel filter and rinsed with PBS+5% FCS (medium I). The tissues were then smashed with a syringe pi...

show abstract

Culture of human parathyroid cells for transplantation

Cited by 8 publications

References 3 publications

Promotion of allogeneic parathyroid cell transplantation in rats with hypoparathyroidism

Promotion of allogeneic parathyroid cell transplantation in rats with hypoparathyroidism

Alotrasplante de paratiroides: seguimiento a 2 años

Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroid adenomas to normocalcemic rabbits

Contact Info

Product

Resources

About