Culture of human mesenchymal stem cells on microcarriers in a 5 l stirred-tank bioreactor

Concise Review: Optimizing Expansion of Bone Marrow Mesenchymal Stem/Stromal Cells for Clinical Applications

“…Collectively, 3D MSC expansion has been performed on hydroxyapatite (HA) [39,42,58], collagen [41], chitosan gelatin and HA/chitosan gelatin [45], PET [44,45], and gelatin microcarriers [43,81]. This varied collection of scaffolds reflects the diverse array of tissues targeted for therapies.…”

“…Perfusion bioreactors have tackled this obstacle by driving culture medium through a scaffold that is tightly fixed to the reactor walls. MSCs have been expanded in all three bioreactors: spinner flasks [38,43,81,83,87], RWVs [40,83], and perfusion bioreactors [39,42,44,46,58] (Table 4). Dynamic culture begets many variables such as cell-seeding density, cell-seeding technique, and cell-expansion method.…”

Mixing theory for culture and harvest in bioreactors of human mesenchymal stem cells on microcarriers

“…In all cases after both culture and harvesting, in order to identify whether there had been any change in the hMSCs as a result of their treatment, they were analysed: 1) according to the International Society of Cell Therapy (ISCT) panel of markers to ascertain their immunophenotypic expression; 2) by tissue-culture adherence and morphology; and 3) for their multilineage differentiation potential [7,8]. All were appropriate showing that the cells maintained the desired quality attributes for therapeutic usage.…”

“…However, once the cells are detached and become freely suspended, damage to the cells from these mechanisms does not occur because they are too small (~15 -18 µm) compared to the Kolmogorov scale [7]. Thus, Equ 2 becomes…”

Cell and gene therapy manufacturing: the necessity for a cost-based development approach

“…However for many clinical indications, achieving lot sizes of several hundred billion to trillions of cells will be imperative for commercial success; utilizing high-density bioreactor manufacturing platforms, such as stirred-tank bioreactors in conjunction with microcarriers, is likely to be the only way to accomplish this [21][22][23][24][25].…”