Culture of Human Mast Cells From Hemopoietic Progenitors

Saito, Hirohisa

doi:10.1385/1-59259-967-2:113

Cited by 11 publications

(10 citation statements)

References 13 publications

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“…IL-3 at 1 ng/ml can enhance the growth of mast cell colonies without inducing granulocyte or macrophage (GM) colonies. At the concentrations above 5 ng/ml, however, IL-3 induces a substantial number of GM colonies [28].…”

Section: Development Of Mast Cells 1 Mast Cells Arise From Multipotementioning

confidence: 95%

“…By contrast, the role of IL-3 on the development of human mast cells is controversial. IL-3 is not required for the development of human cord blood-derived mast cells in the presence of low oxygen concentrations [27], but it can enhance SCF-dependent mast cell development at low cell densities (e.g., in methylcellulose culture) at normal oxygen concentrations [28]. IL-3 at 1 ng/ml can enhance the growth of mast cell colonies without inducing granulocyte or macrophage (GM) colonies.…”

Section: Development Of Mast Cells 1 Mast Cells Arise From Multipotementioning

confidence: 99%

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Development, Migration, and Survival of Mast Cells

2006

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Mast cells play a pivotal role in immediate hypersensitivity and chronic allergic reactions that can contribute to asthma, atopic dermatitis, and other allergic diseases. Because mast cell numbers are increased at sites of inflammation in allergic diseases, pharmacologic intervention into the proliferation, migration, and survival (or apoptosis) of mast cells could be a promising strategy for the management of allergic diseases. Mast cells differentiate from multipotent hematopoietic progenitors in the bone marrow. Stem cell factor (SCF) is a major chemotactic factor for mast cells and their progenitors. SCF also elicits cell-cell and cell-substratum adhesion, facilitates the proliferation, and sustains the survival, differentiation, and maturation, of mast cells. Therefore, many aspects of mast cell biology can be understood as interactions of mast cells and their precursors with SCF and factors that modulate their responses to SCF and its signaling pathways. Numerous factors known to have such a capacity include cytokines that are secreted from activated T cells and other immune cells including mast cells themselves. Recent studies also demonstrated that monomeric IgE binding to FcepsilonRI can enhance mast-cell survival. In this review we discuss the factors that regulate mast cell development, migration, and survival.

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Section: Development Of Mast Cells 1 Mast Cells Arise From Multipotementioning

confidence: 95%

Section: Development Of Mast Cells 1 Mast Cells Arise From Multipotementioning

confidence: 99%

Development, Migration, and Survival of Mast Cells

2006

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“…The relatively low numerical density of mast cells within tissues compromises the number and purity of cells available for in vitro studies. This is especially true for human mast cells that are typically obtained by lengthy tissue digestion and cell purification protocols from surgical specimens [2,3] or derived from peripheral blood or cord-blood progenitor cells [4,5].…”

Section: Introductionmentioning

confidence: 99%

Human mast cell line-1 (HMC-1) cells transfected with FcεRIα are sensitive to IgE/antigen-mediated stimulation demonstrating selectivity towards cytokine production

Xia

Sun

Kuek

et al. 2011

International Immunopharmacology

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“…The latter are based either on in vitro culturing of stem cells, for example, culturing of mouse bone marrow derived MCs (BMMCs), 18 or culturing of human cord blood -derived MCs 19 or in vitro maturation of MCs from peripheral blood. 20 In addition several cell lines have been developed from human and rodent sources including the human HMC-1, LAD2, and LUVA cell lines, [21][22][23] the murine MC/9 cell line 24 and the rat basophilic leukemia cell line, RBL-2H3, herein referred to as RBL. 25 The latter is the focus of this review, where we will summarize the limitations of this model vis-à-vis their value as MC model.…”

mentioning

confidence: 99%

RBL cells as models for in vitro studies of mast cells and basophils

Falcone

Wan

Barwary

et al. 2018

Immunological Reviews

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Since their establishment in 1981, RBL-2H3 cells have been widely used as a mast cell (MC) model. Their ability to be easily grown in culture in large amounts, their responsiveness to FcεRI-mediated triggers and the fact that they can be genetically manipulated, have provided advantages over primary MCs, in particular for molecular studies relying on genetic screening. Furthermore, the ability to generate clones that stably express proteins of interest, for example, a human receptor, have marked the RBL cells as an attractive MC model for drug screening. Indeed, 3 RBL reporter cell lines (RS-ATL8, NFAT-DsRed, and NPY-mRFP) have been generated providing useful models for drug and allergen screening. Similarly, RBL cells stably expressing the human MrgprX2 receptor provide a unique paradigm for analyzing ligand interactions and signaling pathways of the unique human receptor. Finally, transient co-transfections of RBL cells allow functional genomic analyses of MC secretion by combining library screening with simultaneous expression of a reporter for exocytosis. RBL cells thus comprise powerful tools for the study of intracellular membrane trafficking and exocytosis and the detection of allergens, vaccine safety studies and diagnosis of allergic sensitization. Their recent uses as an investigative tool are reviewed here.

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Culture of Human Mast Cells From Hemopoietic Progenitors

Cited by 11 publications

References 13 publications

Development, Migration, and Survival of Mast Cells

Development, Migration, and Survival of Mast Cells

Human mast cell line-1 (HMC-1) cells transfected with FcεRIα are sensitive to IgE/antigen-mediated stimulation demonstrating selectivity towards cytokine production

RBL cells as models for in vitro studies of mast cells and basophils

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