Culture of Human Gingival Fibroblasts: An Experimental Model

Abstract

Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. It is used as a means for in vitro testing of the biocompatibility of resin polymers used in dentistry. The majority of cells are cultured with Dulbecco's modified Eagle's medium (DMEM) or RPMI supplemented with fetal bovine serum. Several cell types are being studied including gingival fibroblasts. Gingival fibroblasts are the main cells of gingival connective tissue. These cells play an active and important role in almost all coating fabric processes, and its involvement in various pathophysiological conditions, including, healing, repair, aging, psoriasis, cancer among others, is only beginning to be understood. DMEM is the most widely used fibroblastic culture medium. This model describes a method for obtaining and cultivating human gingival fibroblasts, by explants derived from surgical discards. Fibroblasts were isolated mechanically and cultured in RPMI 1640 culture medium supplemented with fetal bovine serum 10%, Penicillin (10000 U/ml)/Streptomycin (10 mg/ml) 1% and L-Glutamine (200 mM) 1%. The culture medium is replaced every two days. Cells forming a fairly dense network were observed after a period of 4 days of culture. Human gingival fibroblasts can be cultured by direct explant technique with RPMI 1640 culture medium supplemented with fetal bovine serum and antibiotics.

Chrysin and rutin protect against hydrogen peroxide and tert-butyl hydroperoxide induced oxidative cell damage

“…Human gingival fibroblasts, passage 3, were used to evaluate the cytoprotection potent of rutin and chrysin. Homogenous population of cells had been obtained using the explant culture method as performed previously 14 . The study has been approved by the ethical comity of the odontostomatology department of the Bogodogo's University Hospital Center (Ouagadougou, Burkina Faso).…”

Release of leachable products from resinous compounds in the saliva of children with anterior open bite treated with spur

“…3 Dental materials are susceptible to degradation when applied clinically and, as a consequence, may leach into the oral environment. 2,[4][5][6][7][8] The release of composite resin monomers is potentially hazardous [8][9][10][11] with systemic 11 and local effects on the oral mucosa, gum, and dental pulp. 8,12 One of the products resulting from the decomposition of BisGMA is bisphenol-A (BPA), which is considered a xenoestrogen and can simulate the function of estrogen.…”