Culture of Human Gingival Fibroblasts on a Biodegradable Scaffold and Evaluation of Its Effect on Attached Gingiva: A Randomized, Controlled Pilot Study

Mohammadi, Mohammad Reza; Shokrgozar, Mohammad Ali; Mofid, R

doi:10.1902/jop.2007.070083

Cited by 60 publications

(58 citation statements)

References 26 publications

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“…This is in agreement with further studies using human gingival fibroblasts on a biodegradable collagen scaffold [26,27]. Using these scaffolds a significantly increased amount of attached gingiva at the test site compared to the control site was observed [26]. It is also possible to obtain root coverage without harvesting connective tissue.…”

Section: Surgical Therapy Of Gingival Recessionsupporting

confidence: 88%

“…A complete clinical healing was observed after 2 weeks in 8 out of 10 patients [25]. This is in agreement with further studies using human gingival fibroblasts on a biodegradable collagen scaffold [26,27]. Using these scaffolds a significantly increased amount of attached gingiva at the test site compared to the control site was observed [26].…”

Section: Surgical Therapy Of Gingival Recessionsupporting

confidence: 87%

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New Perspectives in the Diagnostic of Gingival Recession

Dominiak¹,

Gedrange²

2014

Adv Clin Exp Med

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Gingival recession (GR) is a common clinical situation observed in patient populations regardless of their age and ethnicity. It has been estimated that over 60% of the human population has gingival recession. It is the final effect of the interaction of multiple etiological factors. Identification and definition of the range of influence is often not possible, with the result that new methods for testing and elimination of potential etiological factors are still being sought. The aim of this study is to present the etiopathogenesis of gingival recessions with regard to the analysis of morphological and functional factors. For the assessment of the bone factors, we will describe the new cephalometric method for measuring sagital width of the bone in the central incisors area, places when GR are most commonly observed. Also, a review will be presented of modern methods of treatment; in particular classes recessions; usage substitute of autogenous tissue will be emphasized -collagen matrix, and primary culture fibroblasts on collagen net (Adv Clin Exp Med 2014, 23, 6, 857-863).

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Section: Surgical Therapy Of Gingival Recessionsupporting

confidence: 88%

Section: Surgical Therapy Of Gingival Recessionsupporting

confidence: 87%

New Perspectives in the Diagnostic of Gingival Recession

Dominiak¹,

Gedrange²

2014

Adv Clin Exp Med

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“…There are many universities and government research institutes in Iran, such as Royan Institute [24][25][26], Amirkabir University [19,20,[27][28][29][30] , Pasteur Institute of Iran [31][32][33][34], University of Tehran [20,35], Tarbiat Modares University [20,[36][37][38][39], Shaheed Beheshti University of Medical Sciences [40,41,43,45], Tehran University of Medical Sciences [42,44], National Institute for Genetic Engineering [46], Islamic Azad University [47][48][49], Iran University of Science and Technology [50,51] and Iran Polymer and Petrochemical Institute [52,53] are conducting active research on the development of artificial organs based on tissue-engineering technologies.…”

Section: To Presentmentioning

confidence: 99%

“…Because they are differentiated and post-mitotic cell and some cell types have the tendency to de-differentiate during ex vivo cultivation [54]. However, since a study suggested that intra-epidermal injection of dissociated epidermal cells into the lesions of stable vitiligo patients, is an effective, simple and safe therapeutic option for stable vitiligo lesions [55], several researchers in Iran investigated TE with mature cells [25,32].…”

Section: Mature Cellsmentioning

confidence: 99%

Status of tissue engineering and regenerative medicine in Iran and related advanced tools: Bioreactors and scaffolds

Gharravi¹,

Orazizadeh²,

Hashemitabar³

et al. 2012

JBiSE

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“…Les protocoles s'appuyaient sur des matrices collagéniques ensemencées de fibroblastes et une fenestration périostée, des greffes gingivales libres avec thé-rapie cellulaire, des ADMA ensemencées de fibroblastes associées à des greffes de tissu conjonctif (GTC) enfoui sur des récessions, ainsi que des membranes de collagène ensemencées de fibroblastes sous un lambeau déplacé coronairement [5][6][7][8][9]. Ces études comparant les produits d'ingénierie tissulaire (IT) avec les techniques classiques ont montré des résultats satisfaisants en termes d'inflammation postopératoire, de régénération, de couleur et de texture.…”

Section: Introductionunclassified

Étude de faisabilité d’un greffon biofabriqué pour traiter des récessions parodontales

Smirani

Delattre

Kérourédan

et al. 2016

Med Buccale Chir Buccale

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Résumé -Introduction : L'ingénierie tissulaire permet d'envisager de nouvelles thérapeutiques pour le traitement des récessions gingivales. Cette étude de faisabilité proposait un protocole et une chronologie pour la biofabrication de greffons parodontaux sur mesure palliant ces défauts. Technique : L'impression d'une matrice en hydrogel collagénique a été réalisée grâce à un système d'éjection piézoélectrique Microdrop®. La mise au point des conditions optimales d'impression a été déterminée en fabriquant des échantillons de dimension et de morphologie compatibles avec une application clinique. L'ensemencement cellulaire de matrices a été réalisé par bioimpression assistée par laser, et la viabilité cellulaire post-impression testée. L'impression d'une matrice a été directement réa-lisée sur les membranes de collagène préfabriquées pour évaluer la facilité de manipulation du greffon. Les matrices obtenues ont la forme souhaitée, les cellules sont viables, et le greffon est aisément manipulable et suturable. Commentaires : L'impression de la matrice permet de choisir l'épaisseur du greffon. Il est attendu in vivo que l'organisation spatiale des fibroblastes au sein des greffons permette d'augmenter la résistance mécanique et l'esthétique des greffes parodontales. Conclusion : Cette étude de faisabilité préliminaire a permis de poser le concept. D'autres études seront nécessaires pour étudier le remodelage du greffon in vitro et in vivo.Abstract -Feasibility study of biofabricated graft for treating periodontal recession. Introduction: Tissue engineering is a good candidate for treating periodontal recession. This feasibility study proposed a protocol for biofabrication of customized periodontal grafts to treat this problem. Technics: Cell seeding of collagen matrices was done by laser-assisted bioprinting, and post-printing cell viability was assessed. The collagen hydrogel matrix printing was performed by a Microdrop® piezoelectric inkjet system. Optimal conditions were determined by fabricating samples whose dimensions and morphology were suitable for clinical use. Then, the hydrogel matrix printing was carried out directly on collagen membranes to evaluate the ease of the graft handling. The hydrogel matrices had the expected shape, the cells were viable, and the graft was easy to handle and suturable. Comments: The matrix printing allowed one to choose the graft thickness. Fibroblast organization was expected to improve the mechanical resistance and esthetics of periodontal grafts in vivo. Conclusion: This preliminary feasibility study established the bases of the concept. More studies are necessary to assess the graft remodeling both in vitro and in vivo.

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Culture of Human Gingival Fibroblasts on a Biodegradable Scaffold and Evaluation of Its Effect on Attached Gingiva: A Randomized, Controlled Pilot Study

Abstract: Based on the results of this investigation, the tissue-engineered mucosal graft is safe and capable of generating keratinized tissue.

Cited by 60 publications

References 26 publications

New Perspectives in the Diagnostic of Gingival Recession

New Perspectives in the Diagnostic of Gingival Recession

Status of tissue engineering and regenerative medicine in Iran and related advanced tools: Bioreactors and scaffolds

Étude de faisabilité d’un greffon biofabriqué pour traiter des récessions parodontales

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