Culture of Gingival Fibroblasts on Bioabsorbable Regenerative Materials In Vitro

Simain-Sato, Franklin; Lahmouzi, Jamila; Kalykakis, G.; Heinen, Ernst; Defresne, Marie-Paule; Pauw, M. C. De; Grisar, Thierry; Legros, Jean-Jacques; Legrand, Roman

doi:10.1902/jop.1999.70.10.1234

Cited by 13 publications

(25 citation statements)

References 11 publications

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“…In the present study, HGFs were chosen in order to get results closer to normal phenotype behavior in comparison with immortal cell lines (Fukuda et al 2000;Hillmann et al 2002;Locci et al 1997;Payne et al 1996;Simain-Sato et al 1999). Optical microscopy of the bottoms of wells and SEM analysis of the Inion membranes lower face confirmed the absence of cells and a positive barrier effect until 13 weeks, while the manufacturer states an 8-12 weeks in vivo barrier effect.…”

Section: Discussionmentioning

confidence: 84%

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

et al. 2016

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In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to study cell behavior on rigid and flexible/permeable biomaterials elaborating two specific insert-based systems (IBS-R and IBS-F respectively). IBS-R was designed as a specific cylindrical polytetrafluoroethylene (PTFE) system to evaluate attachment, proliferation and morphology of human gingival fibroblasts (HGFs) on grade V titanium and lithium disilicate glassceramic discs characteristics of dental prostheses. The number of cells, their covering on discs and their morphology were determined from MTS assays and microscopic fluorescent images after 24, 48 and 72 h. IBS-F was developed as a two components system to study HGFs behavior on guided bone regeneration polyester membranes. The viability and the membrane barrier effect were evaluated by metabolic MTS assays and by scanning electron microscopy. IBS-R and IBS-F were shown to promote (1) easy and rapid handling; (2) cell retention on biomaterial surface; (3) accurate evaluation of the cellular proliferation, spreading and viability; (4) use of non-toxic material. Moreover IBS-F allowed the study of the cell migration through degradable membranes, with an access to both faces of the biomaterial and to the bottom of culture wells for medium changing.

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Section: Discussionmentioning

confidence: 84%

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

et al. 2016

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“…The use of GTR has proved to be a suitable technique when simulating regeneration, new connective tissue attachment and bone formation [17]. When a membrane is placed between the denuded root surface and the repositioned mucogingival flap, it provides a secluded space into which fibroblasts and osteogenic cells from the healthy apical portion of the periodontium may migrate [15].…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of three bioabsorbable membranes assessed in FGH fibroblasts and human osteoblast like cells culture

Soares

Pereira

et al. 2014

Head Face Med

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ObjectivesSpecific physical and chemical features of the membranes may influence the healing of periodontal tissues after guided tissue regeneration (GTR). The aim of the present investigation was to analyze the biological effects of three bioabsorbable membranes. The hypothesis is that all tested membranes present similar biological effects.MethodsHuman osteoblast like-cells (SaOs-2) and gingival fibroblasts FGH (BCRJ -RJ) were cultured in DMEM medium. The viability of the cells cultured on the membranes was assesses using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Quantitative determination of activated human Transforming Growth Factor beta 1 (TGF-β1) on the supernatants of the cell culture was observed. Samples were examined using scanning electron microscope (SEM).ResultsSaOs2, in 24 hours, PLA group showed higher values when compared to other groups (P < 0.05). All groups presented statistical significance values when compared two times. In 4 h and 24 h, for the fibroblasts group, significantly difference was found to PLA membrane, when compared with the other groups (p < 0.05). For TGFβ1 analyzes, comparing 4 and 24 h, for the osteoblast supernatant, COL1 and PLA groups showed statistically significant difference (p <0,008). On the analysis of culture supernatants of fibroblasts, in 24 hours, only PLA group presented significant difference (p = 0,008).ConclusionsThe biomaterials analyzed did not show cytotoxicity, since no membrane presented lower results than the control group. PLA membrane presented the best performance due to its higher cell viability and absorbance levels of proliferation. Both collagen membranes showed similar results either when compared to each other or to the control group.

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“…GBR sites with well‐integrated unexposed barriers during healing exhibited significantly better bone regeneration than sites with non‐integrated, exposed barriers 40,41 . Adequate tissue integration capacity seems to be intimately related to surface topography, porosity, and chemical composition of the implanted biomaterial 30‐34 . In vitro culture studies have suggested that surface texture, protein binding capacity, and chemical composition influence cellular functions, such as attachment, spreading, morphology, and migration over barrier membranes 23‐26,30‐34 .…”

Section: Discussionmentioning

confidence: 99%

Superficial Topography and Porosity of an Absorbable Barrier Membrane Impacts Soft Tissue Response in Guided Bone Regeneration

Santana

Mattos

Francischone

et al. 2010

Journal of Periodontology

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Culture of Gingival Fibroblasts on Bioabsorbable Regenerative Materials In Vitro

Abstract: These results suggest that cell carrier membranes could affect RGF morphology and thus alter gingival tissue healing following GTR.

Cited by 13 publications

References 11 publications

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

Biocompatibility of three bioabsorbable membranes assessed in FGH fibroblasts and human osteoblast like cells culture

Superficial Topography and Porosity of an Absorbable Barrier Membrane Impacts Soft Tissue Response in Guided Bone Regeneration

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