Culture of gastric biopsies in celiac disease and its relationship with gastritis and Helicobacter pylori infection

Borghini, Raffaele; Donato, Giuseppe; Marino, Mariacatia; Casale, Rossella; Picarelli, Antonio

doi:10.1016/j.dld.2017.10.011

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…One explanation for the temporal change from H pylori‐ positive to H pylori ‐negative in the distribution of pediatric gastritides reflected in the aforementioned studies could be the rising prevalence of pediatric IBD (31–33) and celiac disease (34–36), in which HPNCG is known to be a common finding (5–7,37–39). We found both forms of HPNCG were associated with Crohn disease and the inactive form was also associated with celiac disease and ulcerative colitis.…”

Section: Discussionmentioning

confidence: 99%

“…We found both forms of HPNCG were associated with Crohn disease and the inactive form was also associated with celiac disease and ulcerative colitis. Yet, it should be noted that the prevalence of H pylori-positive gastritides is shown to be higher in IBD and celiac disease patients in other countries (5,7), which may make it difficult to differentiate if the patient's gastritis is related to the Hpylori infection or to the baseline disease. Furthermore, IBD and celiac disease-related symptoms (13,40) were particularly common in patients with HPNCG and diagnoses.…”

Section: Discussionmentioning

confidence: 99%

“…HPNCG remains poorly defined. It has been investigated mainly in connection with selected gastrointestinal conditions, such as inflammatory bowel disease (IBD) and celiac disease (3,(5)(6)(7). The scarcity of studies and often inconsistent biopsy sampling during esophagogastroduodenoscopy (EGD) have hampered the evaluation of the prevalence, main causes, and clinical relevance of HPNCG.…”

Section: What Is Newmentioning

confidence: 99%

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Prevalence and Clinical Significance of Helicobacter Pylori‐negative Chronic Gastritis in Children

Virkkula

Kivelä

Hiltunen

et al. 2022

J. pediatr. gastroenterol. nutr.

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Objectives: The clinical significance of Helicobacter pylori-negative chronic gastritis (HPNCG) in children is unclear. We examined this issue in patients who had undergone esophagogastroduodenoscopy with systematic gastric sampling. Methods: Data of 1178 consecutive children who underwent diagnostic esophagogastroduodenoscopy were collected. Baseline characteristics and long-term outcomes were compared between children with active and inactive HPNCG and those with normal gastric histology. Follow-up data were available for up to 13 years. Results: Altogether 24 (2.0%) children had active and 235 (19.9%) inactive HPNCG, 27 (2.3%) were Hpylori-positive, 46 (3.9%) had other gastric pathology, and 846 (71.8%) normal histology. Diarrhea (31.3% vs 25.1%, P ¼ 0.033), poor growth (23.6% vs 14.7%, P < 0.001), bloody stools (13.9% vs 7.2%, P < 0.001), anemia (46.5% vs 23.4%, P < 0.001), hypersedimentation (39.7% vs 21.4%, P < 0.001), hypoalbuminemia (40.4% vs 16.2%, P < 0.001), and elevated fecal calprotectin (62.4% vs 31.5%, P < 0.001) were more common and heartburn (13.9% vs 22.9%, P ¼ 0.002) less common in the HPNCG group than in the controls. Both active (OR 3.64,95% CI 1.35-9.82) andinactive (2.98, 2.18-4.08) HPNCG predicted a diagnosis in the initial investigations. Crohn disease (41.7%) was the most common diagnosis in active HPNCG and celiac disease (37.4%) in inactive HPNCG. During follow-up, 7 (9.9%) of the 71 initially nondiagnosed HPNCG children received a diagnosis. Conclusions: HPNCG is a frequent finding in children undergoing EGD, the active form being associated especially with Crohn disease and the inactive with celiac disease. The long-term prognosis of patients with HPNCG who do not receive an initial diagnosis is good.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: What Is Newmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and Clinical Significance of Helicobacter Pylori‐negative Chronic Gastritis in Children

Virkkula

Kivelä

Hiltunen

et al. 2022

J. pediatr. gastroenterol. nutr.

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“…Although the presence of t-TG has previously been described in human and animal models of the intestine, 16,17 the presence of t-TG has never to the best of our knowledge been described in the stomach in relation to H. pylori infection. Previous indirect results came from a study by Borghini et al 18 Among 18 patients with celiac disease, the authors did not find significant differences between anti-t-TG levels and anti-endomysium in the supernatant cultures of gastric biopsies separated according to H. pylori status. The apparent contrast with our results may be explained by the different methods used in the studies: the enzyme was detected by using antibodies in the study by Borghini et al, while t-TG activity was measured directly in our study.…”

Section: Discussionmentioning

confidence: 90%

Tissue transglutaminase activity in human gastric mucosa according to Helicobacter pylori infection

Dore

Pes

Errigo

et al. 2018

Exp Biol Med (Maywood)

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Tissue transglutaminase (t-TG) is a multifunctional protein involved in the healing of gastric erosions and ulcers in animal models. The aim of this study was to measure gastric t-TG activity in patients with dyspepsia according to Helicobacter pylori infection and cytotoxin-associated gene A (cagA) and vacuolating cytotoxin (vacA) subtype status. Patients undergoing upper endoscopy not taking any medications were enrolled. Tissue-TG activity was determined in homogenates of antral specimens using a radiometric assay and was expressed in pmol/mg. The cagA and vacA genotypes were determined by PCR amplification using gene-specific oligoprimers. Data from 46 patients were available (17 of them were positive for H. pylori). Antral t-TG activity was significantly increased in H. pylori positive patients compared to H. pylori negative patients (6437 ± 3691 vs. 3773 ± 1530 pmol/mg; P = 0.001) according to Mann–Whitney U test. Patients with H. pylori negative gastritis had higher t-TG activity than patients with normal gastric mucosa. The specimens infected with cagA positive strains (72%) displayed greater t-TG activity than cagA negative samples (7358 ± 4318 vs. 4895 ± 1062 pmol/mg; P = 0.237). Similarly, t-TG activity was higher in H. pylori vacA s1/m1 strains vs. vacA s1/m2 (7429 vs. 5045 pmol/mg; P = 0.744), and vacA s1/m1 vs. s2/m2 (7429 vs. 4489 pmol/mg; P = 0.651) but the results were not significant. No differences were found between histology, endoscopy features and t-TG activity. These results show that t-TG activity is significantly greater in gastritis associated with H. pylori infection, suggesting that this enzyme is induced by inflammation and may have an important role in the natural history of human gastritis. Impact statement Tissue transglutaminase (t-TG) is unique among TG enzymes because of its additional role in several physiological and pathological activities, including inflammation, fibrosis, and wound healing. The presence of t-TG has previously been described in the intestine of human and animal models, yet studies on t-TG activity in human gastric mucosa are missing. Helicobacter pylori infection is the major cause of gastritis and peptic ulcers. For the first time, our results show that t-TG activity was significantly higher in antral specimens of patients with chronic active gastritis associated with H. pylori infection compared to H. pylori negative chronic gastritis and normal antral mucosa. These findings suggest that t-TG has a role in the natural history of human gastritis, which requires further investigation but may be an avenue for new therapeutic options.

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Non-dietary therapies for celiac disease

Yoosuf

Therrien

Leffler

2022

Coeliac Disease and Gluten-Related Disorders

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Culture of gastric biopsies in celiac disease and its relationship with gastritis and Helicobacter pylori infection

Cited by 4 publications

References 9 publications

Prevalence and Clinical Significance of Helicobacter Pylori‐negative Chronic Gastritis in Children

Prevalence and Clinical Significance of Helicobacter Pylori‐negative Chronic Gastritis in Children

Tissue transglutaminase activity in human gastric mucosa according to Helicobacter pylori infection

Non-dietary therapies for celiac disease

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