Culture of cobia (Rachycentron canadum): cryopreservation of sperm and induced spawning

Caylor, Robert E.; Biesiot, Patricia M.; Franks, James S.

doi:10.1016/0044-8486(94)90285-2

Cited by 36 publications

(19 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, a solution with 10% dMSO was the most efficient cryoprotectant, and a similar concentration was suggested for the cryopreservation of red snapper (Riley et al 2004) and mangrove red snapper sperm (Vuthiphandchai et al 2009). In other commercial marine species, satisfactory results were achieved with 10% dMSO for the cryopreservation of cobia Rachycentron canadum (Caylor et al 1994), common snook (Tiersch et al 2004) and fat snook sperm (Tiba et al 2009). The beneficial effect with rapid cooling may also rely on the use of dMSO as the cryoprotectant because dMSO can penetrate sperm cells rapidly and prolonged equilibration during slow cooling can exert more cytotoxicity of dMSO on sperm cells (he et al 2011) Although sperm motility rate is the key parameter in sperm quality, the cryopreservation process should also be evaluated based on the fertilization capacity of oocytes.…”

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Inmentioning

confidence: 99%

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Sanches

Oliveira

Serralheiro

et al. 2013

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders (pH 6.1; 7.8 and 8.2), two concentrations of dimethyl sulfoxide (dMSO, 5 and 10%) and three cooling rates (-90; -60 and -30°C.min ) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes (1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time (P < 0.05) was achieved by combining extender C (ph 8.2) with dMSO (10%) and cooling rate of -60°C.min -1 (P < 0.05). The use of cryopreserved sperm presented fertilization rates higher than 59% validating the present protocol for mutton snapper.

show abstract

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Inmentioning

confidence: 99%

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Sanches

Oliveira

Serralheiro

et al. 2013

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…However, cobia is an excellent candidate for aquaculture because of its rapid growth, reaching 6-10 kg in 12-14 months, a rate around 3-to 5-fold greater than Atlantic salmon, as well as having excellent flesh quality (Liao et al, 2004;Holt et al, 2007). This species also expresses many other favourable production-related characteristics, including spawning in captivity (Caylor et al, 1994;Arnold et al, 2002;Faulk and Holt, 2006), high survival at post-weaning, ability to withstand shifts in salinity (Faulk and Holt, 2006) and responsiveness to vaccination (Lin et al, 2006). Cobia also adapts to confinement and readily accepts commercially-available extruded diets (Craig and McLean, 2005).…”

Section: Introductionmentioning

confidence: 99%

Physiological roles of fatty acyl desaturases and elongases in marine fish: Characterisation of cDNAs of fatty acyl Δ6 desaturase and elovl5 elongase of cobia (Rachycentron canadum)

et al. 2009

View full text Add to dashboard Cite

In the present paper, we investigated the expression of fatty acyl desaturase and elongase genes in a marine teleost, cobia, a species of great interest due to its considerable aquaculture potential. A cDNA was cloned that, when expressed in yeast, was shown to result in desaturation of 18:3n-3 and 18:2n-6, indicating that it coded for a Δ6 desaturase enzyme. Very low desaturation of 20:4n-3 and 20:3n-6 indicated only trace Δ5 activity. Another cloned cDNA enabled elongation of 18:4n-3, 18:3n-6, 20:5n-3 and 20:4n-6 in the yeast expression system, indicating that it had C18-20 and C20-22 elongase activity. Sequence comparison and phylogenetic analysis confirmed that it was homologous to human ELOVL5 elongase. However, the cobia Elovl5 elongase also had low activity toward C24 HUFA. The cobia Δ6 desaturase had a preference for 18:3n-3, but the elongase was generally equally active with both n-3 and n-6 substrates. Expression of both genes was 1-2 orders of magnitude greater in brain than other tissues suggesting an important role, possibly to ensure sufficient docosahexaenoic acid (DHA, 22:6n-3) synthesis in neural tissues through elongation and desaturation of eicosapentaenoic acid (EPA; 20:5n-3).3

show abstract

“…Amphibians may be particularly well suited to such an approach (20). Indeed, cryopreservation of spermatozoa is widely used to propagate species for commercial purposes (4,13,18) and supply gene pools for managing endangered animals (8,9). However, very little is known about the freezing viability of amphibian spermatozoa (2,(21)(22)(23).…”

mentioning

confidence: 99%

Cryopreservation of Spermatozoa from Freeze-Tolerant and -Intolerant Anurans

1998

View full text Add to dashboard Cite

Spermatozoa of the freeze-tolerant wood frog (Rana sylvatica) were used to develop a general protocol for the frozen storage of amphibian spermatozoa. Tolerance of spermatozoa to cryoprotective agents and freezing in suspension (Ϫ80°C) was determined from rates of sperm lysis and dual-fluorochrome vital dye assays. We tested the efficacy of four cryoprotectants (Me 2 SO, methanol, glycerol, and ethylene glycol), two supplements (fetal bovine serum or glutathione), and combinations of these cryoprotectants and supplements. Me 2 SO and fetal bovine serum were the most effective cryoprotectant and supplement, respectively, in reducing sperm lysis. Vital dye assays showed that viability was greatest for spermatozoa treated with both Me 2 SO and fetal bovine serum. Thus, this combination was used to cryopreserve spermatozoa from the freeze-intolerant anurans, Rana pipiens and Bufo americanus. Recovery of viable spermatozoa was significantly greater for R. sylvatica (mean Ϯ SE ϭ 81.2 Ϯ 9.6%) than for R. pipiens (59.0 Ϯ 2.8%) and B. americanus (47.8 Ϯ 4.1%), perhaps owing to inherent factors promoting its freeze tolerance. Nonetheless, our results support the feasibility of using gamete cryopreservation techniques in programs aimed at the captive propagation of amphibians.

show abstract

Culture of cobia (Rachycentron canadum): cryopreservation of sperm and induced spawning

Cited by 36 publications

References 10 publications

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Physiological roles of fatty acyl desaturases and elongases in marine fish: Characterisation of cDNAs of fatty acyl Δ6 desaturase and elovl5 elongase of cobia (Rachycentron canadum)

Cryopreservation of Spermatozoa from Freeze-Tolerant and -Intolerant Anurans

Contact Info

Product

Resources

About