The focus of this study is the movement of the fluid contained in a rectangular bioreactor agitated by line-source bubble plumes. The research included visualization of fluid motion by white polystyrene beads and supported by intensive video recording. Water velocity at the surface and at the bottom, as well as the velocity along the depth, have been measured in two aerated rectangular vessels (36 × 10 −3 m 3 and 2 m 3 in volume) using tap water, as a function of the characteristic velocity (qg)0.33. It was confirmed that the best location of the perforated tube was in the middle of tank and the minimum air flow rate to maintain particle suspension was reached when the aerated surface was a rectangular surface in which the length is twice the width. In addition, the relationship between the fluid velocity, air flow rate, height of the liquid and flow patterns were discussed. Finally, a correlation for the velocity in the liquid was developed and found to be adequate in the scale-up of aerated tanks agitated by an air curtain.Aerated vessels have been widely used in many parts of the world for certain biodegradable industrial waste and wastewater treatment processes. They are also found in chemical engineering as unit operations and as fermenters in biotechnology due to their simple construction, low power requirement and low shear Robinson, 1992a, 1992b). These vessels exist under different design, airlifts, and bubble columns. Recently, Backhurst et al. (1991) introduced for the first time in the field of bioengineering a new type of square tank agitated by an air curtain. In these tanks, the air curtain is created by linesource bubble plumes. Unfortunately, much of the published material on aerated vessels has been concerned with the study of bubble columns and airlift and, therefore, very few papers have dealt with square tanks agitated by an air curtain via a perforated pipe. While aerated vessels agitated by line-source bubble plumes differ from bubble columns and airlift, few publications simply report their use in waste water treatments.In the tanks studied, flow patterns are maintained by an air curtain rising within the vessel. The movement produced by this air curtain interchanges fluid between different parts of the vessel, maintaining particle suspension while promoting gentle mixing. The applications of this type of tank are far reaching in the field of biotechnology.