Culture of 5-day horse embryos in microdroplets for 10 to 20 days

The Effect of Embryo Biopsy and Vitrification on the Development Potential of Equine Embryos

“…The embryos expanded by the third day of culture and later hatched from their zona, rather than the zona thinning and flaking off as occurs in vivo (Hinrichs et al, 1990). According to Hinrichs et al (1990), the results of their study indicate that horse embryos can consistently maintain growth and expansion for 10 days in vitro.…”

“…According to Hinrichs et al (1990), the results of their study indicate that horse embryos can consistently maintain growth and expansion for 10 days in vitro.…”

“…Vitrified embryos can be rapidly warmed within a straw and directly transferred into the uterus of a donor mare (Carnevale, 2006). Hinrichs et al (1990) were the first to describe equine embryo development in long-term culture (10-20 days). The investigators recovered morulae stage embryos via non-surgical lavage and placed them in culture media.…”

“…All 20 embryos were cultured in DMEM/Hams F-12 medium under oil at 37°C in 5% CO 2 in air (Hinrichs et al, 1990). Embryos were monitored for expansion and hatching.…”

“…The twenty embryos were divided into a biopsy (n=10) and control group (n=10). The biopsy group underwent microaspiration biopsy using a micromanipulator to obtain a small tissue sample from the embryo.Both groups were then vitrified using a commercially available technique originally described by Carnevale (2006) at Colorado State University.All 20 embryos were cultured in DMEM/Hams F-12 medium under oil at 37°C in 5% CO 2 in air (Hinrichs et al, 1990). Embryos were monitored for expansion and hatching.…”