Culture Methods for Neisseria Gonorrhoea

Kraus, Stephen J.

doi:10.3109/01485017908988426

Cited by 9 publications

(3 citation statements)

References 32 publications

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“…Another disadvantage of the method is the need of more days for the cultivation process. Some-thing else, gonorrhea-selective culture medium has progressively developed, but the antibiotics which have contributed to the improvement may occasionally inhibit certain sensitive strains (9).…”

Section: Discussionmentioning

confidence: 99%

Detection of common bacterial causes of urethritis in symptomatic men at the STD laboratory of MU-Sofia by microscopy, culture and NAAT

Raykova

2019

SSM

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INTRODUCTION: Urethritis is one of the major causes of morbidity in men. The primary pathogens are Chlamydia trachomatis and Neisseria gonorrhoeae, but also Mycoplasma genitalium. Ureaplasma urealyticum, Trichomonas vaginalis, anaerobes, Herpes simplex virus (HSV), and adenovirus. AIM: The aim of this study was to detect common bacterial causes of urethritis in symptomatic men by Gram stain, culture and nucleic acid amplification techniques (NAAT), and to compare them. MATERIALS AND METHODS: Seventy-eight male patients with clinical symptoms suggestive of urethritis were enrolled in the study. Three urethral samples were obtained from each one -for smear on a glass slide, culture, and NAAT. The glass slides were subjected to Gram stain. Culture on modified Thayer Martin media was used for detection of N. gonorrhoeae. Isolation of C. trachomatis was performed on McCoy cells, afterwards stained for immunofluorescence with anti-lipopolysaccharide monoclonal antibody. Cultivation and ennumeration of Ureaplasma spp. and M. hominis was done with Mycofast Revolution kit. DNA extractions and amplifications by using multiplex Real Time PCR tests were done for all the bacteria. RESULTS: In 30 persons, infections were detected by using different microbiological tests. N. gonorrhoeae was discovered by Gram stain in 5 samples; by cultivation -in 6; by PCR -in 8. C. trachomatis was found in 13 samples with cultivation; by PCR -in 14. Ureaplasma spp. was found in 7 samples with cultivation and in 9 with PCR. M. genitalium was detected only by PCR in 3 samples. M. hominis was negative in all tested swabs. Co-infections with two microorganisms were detected in 4 samples. All the samples with positive results showed increased number of leukocytes on Gram staining. DISCUSSION: Although many infections can be diagnosed on the basis of clinical criteria alone, accurate and timely diagnostic microbiology is essential for the clinical management of patients' infections. In our study the PCR was the most sensitive and rapid method for the diagnosis of urethritis in symptomatic

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Section: Discussionmentioning

confidence: 99%

Detection of common bacterial causes of urethritis in symptomatic men at the STD laboratory of MU-Sofia by microscopy, culture and NAAT

Raykova

2019

SSM

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“…Dengan hasil oksidase-positif, diplokokus Gram-negatif didapatkan dari 100% kultur sampel yang dikirim dalam satu hari, 67% setelah 2 hari, dan 17% setelah 3 hari. Koloni tetap menghasilkan oksidase-positif bahkan setelah 3 hari, tetapi terdapat gonokokus yang autolysis pada pewarnaan gram (Kraus, 2009…”

Section: Pendahuluanunclassified

Pengaruh Lama Waktu Inkubasi Terhadap Morfologi Bakteri Neisseria gonorrhoeae

Adawiyah

Diarti

Tatontos

2020

JURNAL KESEHATAN POLTEKKES KEMENKES RI PANGKALPINANG

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Lama waktu yang digunakan untuk menginkubasi bakteri akan mempengaruhi pertumbuhan bakteri tersebut secara makroskopis dan mikroskopis. Biakan bakteri pada kondisi inkubasi yang lama atau diatas waktu optimum yang diperlukan oleh bakteri untuk tumbuh akan mempengaruhi morfologi bakteri secara mikroskopis, bentuk bakteri akan berbeda dari bentuk dasarnya sehingga tidak dapat diamati dengan jelas karena pengaruh lama waktu inkubasi yang digunakan. Penelitian ini bertujuan untuk mengetahui adanya pengaruh lama waktu inkubasi terhadap morfologi bakteri Neisseria gonorrhoeae, penelitian ini bersifat pre-eksperiment yang menggunakan biakan murni bakteri Neisseria gonorrhoeae (gram negatif) dengan waktu inkubasi 24, 48, 72, dan 96 jam. Hasil preparat bakteri Neisseria gonorrhoeae diperoleh bentuk mikroskopis bakteri coccus pada lama waktu inkubasi 24 dan 48 jam dengan kategori baik. Dari hasil penelitian dapat ditarik kesimpulan bahwa lama waktu inkubasi yang baik digunakan untuk mengamati morfologi bakteri Neisseria gonorrhoeae pada lama waktu inkubasi 24 dan 48 jam.

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“…Neisseria spp. are carboxyphilic (capnophilic) bacteria; they require an atmosphere composed of oxygen and a raised level of CO 2 and therefore grow best in an atmosphere enriched with 5 -10% CO 2 (6,7,8,9). This atmosphere can be provided using a CO 2 incubator, which adds CO 2 gas via a regulator, or by using a CO 2 generating GasPak kit or candle in a culture jar.…”

Section: Introductionmentioning

confidence: 99%

Improvement ofNeisseria gonorrhoeaeculture media to enable growth without CO₂

Snyder

Menkiti

2023

Preprint

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Bacterial culture is the crucial step in diagnosingNeisseria gonorrhoeaeinfections and is the gold standard for determining their antimicrobial resistance profile. However, culture ofNeisseriaspp. can be challenging in resource poor areas, relying on specialist incubators supplying 5% CO2for growth of the bacteria. Even when such incubators are available, the CO2to run them may be scarce; there were CO2shortages during the COVID-19 pandemic, for example. Although culture jars with gas packs or candles can be used, these are inefficient in terms of use of incubator space and researcher time. To achieve simplicity in culturing ofN. gonorrhoeae, the standard Oxoid GC base medium, made with the Kellogg’s glucose and iron supplements was improved with the addition of 0.75 g/L sodium bicarbonate (NaHCO3), which is inexpensive and really available. This improved media was able to sustain gonococcal growth as well as standard GC media in 5% CO2. Chocolate agar and Thayer-Martin agar with sodium bicarbonate was also developed, with all showing good growth ofN. gonorrhoeaewithout the need for atmospheric CO2.

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Culture Methods for Neisseria Gonorrhoea

Cited by 9 publications

References 32 publications

Detection of common bacterial causes of urethritis in symptomatic men at the STD laboratory of MU-Sofia by microscopy, culture and NAAT

Detection of common bacterial causes of urethritis in symptomatic men at the STD laboratory of MU-Sofia by microscopy, culture and NAAT

Pengaruh Lama Waktu Inkubasi Terhadap Morfologi Bakteri Neisseria gonorrhoeae

Improvement ofNeisseria gonorrhoeaeculture media to enable growth without CO₂

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Culture Methods for Neisseria Gonorrhoea

Cited by 9 publications

References 32 publications

Detection of common bacterial causes of urethritis in symptomatic men at the STD laboratory of MU-Sofia by microscopy, culture and NAAT

Detection of common bacterial causes of urethritis in symptomatic men at the STD laboratory of MU-Sofia by microscopy, culture and NAAT

Pengaruh Lama Waktu Inkubasi Terhadap Morfologi Bakteri Neisseria gonorrhoeae

Improvement ofNeisseria gonorrhoeaeculture media to enable growth without CO2

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Improvement ofNeisseria gonorrhoeaeculture media to enable growth without CO₂