Culture medium pH and stationary phase/chronological aging of different cells

Моргунова, Г. В.; Клебанов, А. А.; Marotta, F; Хохлов, А. Н.

doi:10.3103/s0096392517020109

Cited by 19 publications

(10 citation statements)

References 36 publications

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“…[56][57][58] Chronological aging implies restriction of cell proliferation by some way (preferably by contact inhibition) with further determination of survivability of cells in stationary phase of growth; replicative aging is based on the phenomenon of limited cell proliferation potential. 59,60 Chronological aging is also called stationary phase aging, the latter term being used more often for bacteria 61 and mammalian cells. [62][63][64] Stationary conditions contribute AMPK activation and autophagy initiation, thus investigation of the enzyme's regulatory role in the chronological aging model is of special interest.…”

Section: Caloric Restriction Extends Lifespan In Yeasts In Both Chronmentioning

confidence: 99%

Age‐related AMP‐activated protein kinase alterations: From cellular energetics to longevity

Моргунова

Клебанов

2019

Cell Biochemistry & Function

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Funding informationRussian Foundation for Basic Research, Grant/ Award Number: 18-34-00813 mol_a 5' adenosine monophosphate-activated protein kinase (AMPK) is a key regulator of energy in the cell, which allows the cell/organism to survive with deficit of ATP. Since AMPK is involved in the adaptation to caloric restriction, the role of age-related changes in AMPK activity in both the aging organism and the aging cell is actively investigated in gerontology. Studies on yeast, worms, flies, rodents, and primates have demonstrated an important effect of this regulator on key signalling pathways involved in the aging process. In some cases, researchers conclude that AMPK promotes aging. However, in our opinion, in such cases, we observe a disturbance in the adaptive ability because of the prolonged cell/organism presence in stressful conditions because the functional capacity of any adaptation system is limited. Interestingly, AMPK can regulate metabolic processes in noncell-autonomous manner. The main effects of AMPK activation in the cell are realized in restriction of proliferation and launching autophagy. In tissues of an aging organism, the ability of AMPK to respond to energy deficit decreases; this fact is especially critical for organs that contain postmitotic cells. In this review, we have tried to consider the involvement of AMPK in age-related changes in the cell and in the organism.

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Section: Caloric Restriction Extends Lifespan In Yeasts In Both Chronmentioning

confidence: 99%

Age‐related AMP‐activated protein kinase alterations: From cellular energetics to longevity

Моргунова

Клебанов

2019

Cell Biochemistry & Function

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“…Такое «плечо» возникает в некоторых исследованиях, где изменяют условия культивирования, например, добавляют буфер [23] или изменяют состав питательных сред [11]. Это означает, что только при определённых условиях можно говорить о «старении» дрожжей [24], во многих случаях экспериментаторы изучают вымирание от других причин -истощение питательных веществ или отравление продуктами распада, но не старение.…”

Section: материалы и методыunclassified

Demographic Approaches to the Study of Aging on Cell Cultures

Хохлов

Моргунова

Клебанов

2019

Moscow Univ. Biol.Sci. Bull.

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“…При хронологическом старении дрожжей и "стационарном" старении культивируемых клеток млекопитающих происходит ограничение пролиферации вследствие контактного торможения, в результате чего клетки не обновляются и в них происходят разного рода изменения, сходные с изменениями, характерными для стареющих многоклеточных организмов. Наиболее важные среди них -повреждения ДНК, так как любые другие макромолекулы и органеллы можно заменить [24][25][26]. Ранее было показано, что в условиях этой модельной системы в клетках возникают сшивки ДНК-белок [27] и однонитевые разрывы [28], происходит деметилирование ДНК [29] и ингибирование процесса поли(AДФ-рибозил)ирования белков хроматина [30], изменяется уровень спонтанных сестринских хроматидных обменов [31,32].…”

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