Culture medium modulates the behaviour of human dental pulp-derived cells: Technical Note

Lopez-Cazaux, Séréna; Bluteau, Gilles; Magne, David; Lieubeau, Blandine; Guicheux, Jérôme; Alliot‐Licht, Brigitte

doi:10.22203/ecm.v011a05

Cited by 45 publications

(41 citation statements)

References 41 publications

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“…Interestingly, the pre-incubation with valproic acid induced morphological and growth changes in DPSCs resulting in retraction and spontaneous formation of multilayered nodules. Such cell dynamic process was not observed without pre-treatment nevertheless similar growing pattern was previously reported for DPSCs cultured under high-serum conditions (14) or cultures of perivascular pericytes (19). In addition bFGF, which is known for its effect potentiating osteogenesis in mesenchymal stem cells (16,18), seems to have synergic effect with valproic acid on osteodifferentiation.…”

Section: Discussionsupporting

confidence: 78%

Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions

Karbanová

Soukup

Suchánek

et al. 2010

Acta Med. (Hradec Kralove, Czech Repub.)

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Summary:Dental pulp stem cells (DPSCs) can be easily isolated and cultured in low-serum containing medium supplemented with growth factors PDGF-BB and EGF while exhibiting multipotency and immature phenotypic characteristics. In the present study, we investigated their potential to differentiate towards osteogenic lineages using various culture conditions in order to optimize their therapeutic use. DPSCs were cultured either as a cell monolayer or as three-dimensional (3D) micro-mass structures. Monolayers preincubated with bFGF and valproic acid for one week prior their differentiation were cultured in serum containing standard osteodifferentiation medium for four weeks, which resulted in multilayered nodule formation. Micro-mass structures were cultured for same period either in serum containing medium or under serum-free conditions supplemented with TGF-ß3 with or without BMP-2. Histochemically, we detected massive collagen I and weak calcium phosphate depositions in multilayered nodules. When culture 3D-aggregates in either standard osteodifferentiation medium or serum-free medium containing TGF-ß3, only small amount of collagen I fibres was observed and almost no deposits of calcium phosphate were detected. In contrast, in presence of both TGF-ß3 and BMP-2 in the serum-free medium a significant amount of collagen I fibers/bundles and calcification were detected, which is in line with osteogenic effect of BMP-2. Thus, our data indicate that certain environmental cues can enhance differentiation process of DPSCs into osteogenic lineage, which suggest their possible utilization in tissue engineering.

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Section: Discussionsupporting

confidence: 78%

Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions

Karbanová

Soukup

Suchánek

et al. 2010

Acta Med. (Hradec Kralove, Czech Repub.)

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“…This current study demonstrated that the most appropriate culture media are α-MEM and DMEM supplemented with 20% FBS based on in vivo doubling time of epithelial cells within 5 days [30]. This result disagrees with the results reported by Whitehead (1:1) culture media compared with colon epithelial cells cultivated in α-MEM and DMEM culture media [46].…”

Section: Discussioncontrasting

confidence: 99%

In Vitro Sustained Differentiation of Rat Colon Epithelial Stem Cells

Abu‐Serie¹,

Demellawy²,

El‐Sayed³

et al. 2016

Biochem Anal Biochem

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Colonic epithelium composes of various cell types including alkaline phosphate-expressing absorptive, mucussecreting goblet and neuroendocrine cells that are derived from stem cells through asymmetric division. The continuous renewal of stem cells occurs under the highly coordinated cellular redox state. In the current study, based on a comparison with other culture media, colon epithelial cells were able to be sustained in vitro with normal status for more than two months under the chosen culture condition; α-MEM medium containing 20% fetal bovine serum. The cultured epithelial cells had normal doubling time and normal morphological characteristics as examined by transmission electron microscope. Also, these cultured cells contained functional stem cells and maintained their differentiation potency of colon stem cells, compared with freshly isolated mucosal epithelial cells, as indicated by the maintaining of aldehyde dehydrogenase 1B1 expression (11.31 ± 0.45 to 11.15 ± 0.48), ability to reduce silver nitrate, alkaline phosphate activity (0.513 ± 0.007 mU/μg to 0.438 ± 0.005 mU/μg), mucin secretion (34.71 ± 0.714 μg/ml to 32.93 ± 0.357 μg/ml) in appropriate cellular redox state level (-258.4 ± 1.3 mV to -237.4 ± 3.7 mV). The present study showed sustaining replication potential and functional differentiation of colonic epithelial stem cell population in this culture. The above culture system may be useful as an in vitro model for stemness, toxicological, and carcinogenesis studies.

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“…MEM is the most commonly used medium among others in cellular culture and is considered to have a low concentration of phosphate (1mM) and glucose (1g/L) and high calcium concentration (1.8mM) (Lopez-Cazaux et al 2006). Low glucose DMEM is unique among other media due to the elevated amino acid and vitamins concentrations, which are four times higher than MEM, while possessing the same levels of glucose, calcium and phosphate.…”

Section: Discussionmentioning

confidence: 99%

Potential for in vitro mesoderm differentiation of Wharton's jelly cells from ovine umbilical cord isolated in different culture media

et al. 2016

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RESUMO.-[Potencial de diferenciação in vitro de cé-lulas provenientes da geleia de Wharton de cordões umbilicais ovinos isolados em diferentes meios de cultivo.] A geleia de Wharton do cordão umbilical (GWCU) de mamíferos é uma fonte promissora de células multipotentes, sendo vantajosa por aspectos éticos, facilidade de coleta e não causar teratomas em ensaios pré-clínicos. Em ovinos, células multipotentes já foram isoladas de vários tecidos, no entanto, não existem relatos do isolamento a partir do cordão umbilical nesta espécie. O objetivo do presente estudo foi investigar o melhor meio para o transporte do cordão umbilical, isolar e manter as células da GWCU The mammalian Wharton's jelly of umbilical cord (WJUC) is a promising source of multipotent cells, providing advantages due to ethical implications, ease of collection and the absence of teratomas in pre-clinical trials. Ovine multipotent cells have already been isolated from various tissues, however there are no reports using umbilical cords in this species. This study aimed to investigate the best medium to transport the umbilical cord, to isolate and maintain ovine WJUC cells and to compare in vitro growth and mesodermal differentiation potential. Eight ovine umbilical cords were obtained during parturition, sectioned and transported in six different media: MEM, low glucose DMEM, M199, RPMI 1640, PBS and saline. For each transportation medium, four culture media were used and the tissue was explanted in 24-well plates and cultured in MEM, low glucose DMEM, M199 and RPMI 1640, all with 10% FBS. Every experiment was conducted with low-passage (P2), investigating MTT viability during four days and adipogenic, chondrogenic and osteogenesis differentiation was induced in vitro. The most effective transport medium (p<0.1) was low glucose DMEM. There was no bacterial or fungal contamination from collection. Cells from Wharton's jelly of ovine umbilical cords collected at natural birth possess fibroblastic morphology and the capacity for in vitro differentiation into adipogenic, chondrogenic and osteogenic cell lines. MTT tests and in vitro differentiation experiments revealed that cell culture medium modulates the behavior of cells and is an important factor for proliferation and maintenance of multipotency. Low glucose DMEM was the most suitable medium for the isolation of cells from Wharton's jelly of ovine umbilical cord.

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Culture medium modulates the behaviour of human dental pulp-derived cells: Technical Note

Cited by 45 publications

References 41 publications

Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions

Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions

In Vitro Sustained Differentiation of Rat Colon Epithelial Stem Cells

Potential for in vitro mesoderm differentiation of Wharton's jelly cells from ovine umbilical cord isolated in different culture media

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