Culture Medium Modulates Proinflammatory Conditions of Human Pancreatic Islets Before Transplantation

Marzorati, Simona; Antonioli, Barbara; Nano, Rita; Maffi, Paola; Piemonti, Lorenzo; Giliola, C.; Secchi, Antonio; Lakey, Jonathan R.T.; Bertuzzi, Federico

doi:10.1111/j.1600-6143.2006.01512.x

Cited by 44 publications

(33 citation statements)

References 18 publications

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“…Treatment of islets with antioxidants [164,166,167] or anti-inflammatory agents [168,169] promotes islet survival by reducing the infiltration of macrophages and production of proinflammatory cytokines [170]. The protease inhibitor a1-antitrypsin inhibits thrombin, and promotes early graft survival [171].…”

Section: Loss Of Beta Cells During Islet Isolation and Primary Non-fumentioning

confidence: 99%

Beta cell apoptosis in diabetes

et al. 2009

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Apoptosis of beta cells is a feature of both type 1 and type 2 diabetes as well as loss of islets after transplantation. In type 1 diabetes, beta cells are destroyed by immunological mechanisms. In type 2 diabetes abnormal levels of metabolic factors contribute to beta cell failure and subsequent apoptosis. Loss of beta cells after islet transplantation is due to many factors including the stress associated with islet isolation, primary graft non-function and allogeneic graft rejection. Irrespective of the exact mediators, highly conserved intracellular pathways of apoptosis are triggered. This review will outline the molecular mediators of beta cell apoptosis and the intracellular pathways activated.

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Section: Loss Of Beta Cells During Islet Isolation and Primary Non-fumentioning

confidence: 99%

Beta cell apoptosis in diabetes

et al. 2009

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“…In the study by Winter et al [42] , the combined administration of vitamin C and E, but not vitamin A and selenium, led to a significant improvement in functional islet graft survival, associated with augmented islet engraftment. In contrast, Brown et al [40] and Marzorati et al [26] showed that the combination of vitamin E and C was unable to improve graft survival or decrease in-vitro MCP-1 release, respectively.…”

mentioning

confidence: 94%

“…metabolites and enzyme systems as well as reacting directly with oxidants in the cells. When GSH was added to culture medium, in contrast to vitamin E and C, it reduced MCP-1 [26] . L-glutamine as a precursor of GSH was shown to increase islet yield when added intra-ductally during human pancreatic islet isolation.…”

Section: Rat → Ratmentioning

confidence: 99%

“…Table 1 lists the various antioxidant agents which have been added to islets during the isolation procedure or to the culture medium. [26] , 2006 [29] , 2005 L-glutamine (5 mmol/L) Human Pancreas were manually perfused through the main pancreatic duct with either the standard HBSS or with HBSS + L-glutamine…”

Section: Antioxidant Recruitment In Islet Transplantation Studiesmentioning

confidence: 99%

“…In a different study [26] , the use of vitamin E and ascorbic acid did not decrease the release of monocyte chemoattractant protein 1 (MCP-1) from human islets. MCP-1 is a chemokine secreted from pancreatic islets to recruit the immune system and plays an important role in the clinical outcome of islet transplantation because of its proinflammatory property [47,48] .…”

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confidence: 99%

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Islet transplantation and antioxidant management: A comprehensive review

Monfared¹,

Larijani

Abdollahi

2009

WJG

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Islet transplantation as a promising treatment for type 1 diabetes has received widespread attention. Oxidative stress plays an essential role in cell injury during islet isolation and transplantation procedures. Antioxidants have been used in various studies to improve islet transplantation procedures. The present study reviews the role of oxidative stress and the benefits of antioxidants in islet transplantation procedures. The bibliographical databases Pubmed and Scopus were searched up to November 2008.All relevant human and animal in-vivo and in-vitro studies, which investigated antioxidants on islets, were included. Almost all the tested antioxidants used in the in-vitro studies enhanced islet viability and insulin secretion. Better control of blood glucose after transplantation was the major outcome of antioxidant therapy in all in-vivo studies. The data also indicated that antioxidants improved islet transplantation procedures. Although there is still insufficient evidence to draw definitive conclusions about the efficacy of individual supplements, the benefits of antioxidants in islet isolation procedures cannot be ignored.

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The potential role of multifunctional human amniotic epithelial cells in pancreatic islet transplantation

Murray

Zafar

Qureshi

et al. 2021

J Tissue Eng Regen Med

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Pancreatic islet cell transplantation has proven efficacy as a treatment for type 1 diabetes mellitus, chiefly in individuals who are refractory to conventional insulin replacement therapy. At present its clinical use is restricted, firstly by the limited access to suitable donor organs but also due to factors associated with the current clinical transplant procedure which inadvertently impair the long‐term functionality of the islet graft. Of note, the physical, biochemical, inflammatory, and immunological stresses to which islets are subjected, either during pretransplant processing or following implantation are detrimental to their sustained viability, necessitating repeated islet infusions to attain adequate glucose control. Progressive decline in functional beta (β)‐cell mass leads to graft failure and the eventual re‐instatement of exogenous insulin treatment. Strategies which protect and/or preserve optimal islet function in the peri‐transplant period would improve clinical outcomes. Human amniotic epithelial cells (HAEC) exhibit both pluripotency and immune‐privilege and are ideally suited for use in replacement and regenerative therapies. The HAEC secretome exhibits trophic, anti‐inflammatory, and immunomodulatory properties of relevance to islet graft survival. Facilitated by β‐cell supportive 3D cell culture systems, HAEC may be integrated with islets bringing them into close spatial arrangement where they may exert paracrine influences that support β‐cell function, reduce hypoxia‐induced islet injury, and alter islet alloreactivity. The present review details the potential of multifunctional HAEC in the context of islet transplantation, with a focus on the innate capabilities that may counter adverse events associated with the current clinical transplant protocol to achieve long‐term islet graft function.

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Culture Medium Modulates Proinflammatory Conditions of Human Pancreatic Islets Before Transplantation

Cited by 44 publications

References 18 publications

Beta cell apoptosis in diabetes

Beta cell apoptosis in diabetes

Islet transplantation and antioxidant management: A comprehensive review

The potential role of multifunctional human amniotic epithelial cells in pancreatic islet transplantation

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