Culture Medium and Protein Supplementation in the Generation and Maturation of Dendritic Cells

Royer, Pierre‐Joseph; Tanguy-Royer, Séverine; Ebstein, Frédéric; Sapede, C.; Thézenas, Simon; Barbieux, Isabelle; Oger, Romain; Grégoire, Marc

doi:10.1111/j.1365-3083.2006.001757.x

Cited by 29 publications

(23 citation statements)

References 30 publications

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“…In a previous study we demonstrate that the combination of TNF-α and poly I:C is the most effi cient in inducing the complete activation of human DCs compared to a large variety of maturation molecules or cytokine cocktails (Spisek, 2001;. These two molecules are also relevant for clinical applications of this maturation process (Mc IlRoy et al, 2003;Royer et al, 2006). In this study, we show that DC maturation requires second signals delivered by activated CD4+ T cells a few hours after exposure to peripheral maturation stimuli to fully initiate CD8+ T cell responses.…”

Section: Discussionmentioning

confidence: 99%

Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses

et al. 2012

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Dendritic cells (DCs) are professional antigen-presenting cells involved in the control and initiation of immune responses. In vivo, DCs exposed at the periphery to maturation stimuli migrate to lymph nodes, where they receive secondary signals from CD4+ T helper cells. These DCs become able to initiate CD8+ cytotoxic T lymphocyte (CTL) responses. However, in vitro investigations concerning human monocyte-derived DCs have never focused on their functional properties after such sequential maturation. Here, we studied human DC phenotypes and functions according to this sequential exposure to maturation stimuli. As fi rst signals, we used TNF-α/polyI:C mimicking infl ammatory and pathogen stimuli and, as second signals, we compared activated CD4+ T helper cells to a combination of CD40-L/ IFN-γ. Our results show that a sequential activation with activated CD4+ T cells dramatically increased the maturation of DCs in terms of their phenotype and cytokine secretion compared to DCs activated with maturation stimuli delivered simultaneously. Furthermore, this sequential maturation led to the induction of CTL with a long-term effector and central memory phenotypes. Thus, sequential delivery of maturation stimuli, which includes CD4 + T cells, should be considered in the future to improve the induction of long-term CTL memory in DC-based immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses

et al. 2012

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“…Several media meet the guidelines of GMP and can be used for DC generation. Successful studies have been performed using CellGro media (Cellgenix) or X-VIVO media (Lonza) (Schuler-Thurner et al, 2000; Royer et al, 2006; Schadendorf et al, 2006; Tuettenberg et al, 2006). Importantly, depending on local regulations for GMP media, there are major differences in the quality of generated DC.…”

Section: Protocols For Generation Of Tolerogenic and Immunogenic Dendmentioning

confidence: 99%

Costimulatory Molecules on Immunogenic Versus Tolerogenic Human Dendritic Cells

Hubo¹,

Trinschek²,

et al. 2013

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Dendritic cells (DC) are sentinels of immunity, essential for homeostasis of T cell-dependent immune responses. Both functions of DC, initiation of antigen-specific T cell immunity and maintenance of tissue-specific tolerance originate from distinct stages of differentiation, immunogenic versus tolerogenic. Dependent on local micro milieu and inflammatory stimuli, tissue resident immature DC with functional plasticity differentiate into tolerogenic or immunogenic DC with stable phenotypes. They efficiently link innate and adaptive immunity and are ideally positioned to modify T cell-mediated immune responses. Since the T cell stimulatory properties of DC are significantly influenced by their expression of signal II ligands, it is critical to understand the impact of distinct costimulatory pathways on DC function. This review gives an overview of functional different human DC subsets with unique profiles of costimulatory molecules and outlines how different costimulatory pathways together with the immunosuppressive cytokine IL-10 bias immunogenic versus tolerogenic DC functions. Furthermore, we exemplarily describe protocols for the generation of two well-defined monocyte-derived DC subsets for their clinical use, immunogenic versus tolerogenic.

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“…For instance, batch-to-batch variability of serum or feeder cells can influence the characteristics of in vitro differentiated DCs. [13] Here, we describe a novel serum- and feeder cell-free method that robustly and repetitively produces monocytic lineage cells from human ESCs/iPSCs.…”

Section: Introductionmentioning

confidence: 99%

Robust and Highly-Efficient Differentiation of Functional Monocytic Cells from Human Pluripotent Stem Cells under Serum- and Feeder Cell-Free Conditions

et al. 2013

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Monocytic lineage cells (monocytes, macrophages and dendritic cells) play important roles in immune responses and are involved in various pathological conditions. The development of monocytic cells from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is of particular interest because it provides an unlimited cell source for clinical application and basic research on disease pathology. Although the methods for monocytic cell differentiation from ESCs/iPSCs using embryonic body or feeder co-culture systems have already been established, these methods depend on the use of xenogeneic materials and, therefore, have a relatively poor-reproducibility. Here, we established a robust and highly-efficient method to differentiate functional monocytic cells from ESCs/iPSCs under serum- and feeder cell-free conditions. This method produced 1.3×106±0.3×106 floating monocytes from approximately 30 clusters of ESCs/iPSCs 5–6 times per course of differentiation. Such monocytes could be differentiated into functional macrophages and dendritic cells. This method should be useful for regenerative medicine, disease-specific iPSC studies and drug discovery.

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Culture Medium and Protein Supplementation in the Generation and Maturation of Dendritic Cells

Cited by 29 publications

References 30 publications

Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses

Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses

Costimulatory Molecules on Immunogenic Versus Tolerogenic Human Dendritic Cells

Robust and Highly-Efficient Differentiation of Functional Monocytic Cells from Human Pluripotent Stem Cells under Serum- and Feeder Cell-Free Conditions

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