Culture media for the isolation of Cronobacter spp

Druggan, Patrick; Iversen, Carol

doi:10.1016/j.ijfoodmicro.2009.09.008

Cited by 48 publications

(34 citation statements)

References 66 publications

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“…However, this value may be an underestimate, as the method specified in ISO/TS 22964 was used for isolation as the best alternative available in 2006, when the study has started. This method is now known to miss some strains unable to grow in mLST media and/or at 45°C (Iversen and Forsythe 2007;Druggan and Iversen 2009). Advanced procedures have been recently recommended for effective isolation of Cronobacter spp., e.g.…”

Section: Discussionmentioning

confidence: 99%

“…identification method (Druggan and Iversen 2009). In our study it was used as a principal identification test for Cronobacter genus confirmation and it 100% agreed with AFLP and 16S rRNA sequencing classification.…”

Section: Discussionmentioning

confidence: 99%

“…Commercial biochemical systems or PCR-based assays are available for food and clinical microbiological laboratories (Fanjat et al 2007;Stoop et al 2009;Druggan and Iversen 2009). Generally, phenotype analysis may provide unreliable identification as long as it is based on unstable expression of the markers (Drudy et al 2006;Druggan and Iversen 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Generally, phenotype analysis may provide unreliable identification as long as it is based on unstable expression of the markers (Drudy et al 2006;Druggan and Iversen 2009).…”

Section: Introductionmentioning

confidence: 99%

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Biochemical and molecular characterization of Cronobacter spp. (formerly Enterobacter sakazakii) isolated from foods

Turcovský

Kuniková

Drahovská

et al. 2010

Antonie van Leeuwenhoek

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The aim of this study was to identify and characterize Cronobacter spp. isolated from a range of foods. A total of 71 Cronobacter strains were isolated from 602 foods in our laboratory. The highest contamination was observed in foods of plant origin, e.g. spices, teas, chocolate, nuts, pastries and vegetables. On the basis of genus and species identification performed using genus-specific PCR, 16S rRNA sequencing and AFLP genotyping, most of the strains belonged to Cronobacter sakazakii. Biochemical profiling by the tests included in API 20E, complemented with relevant additional tests, classified the strains into 13 biogroups. AFLP genotyping facilitated discrimination of six main groups at the 70% similarity level and strain grouping correlated clearly with species identification. Our results indicate that molecular typing by AFLP may be applied as a useful tool not only for direct comparison of Cronobacter isolates, providing traceability, but also for the reliable species classification. Moreover, tracing of these bacteria in a wider variety of foods should be important to enhance the knowledge of their transmission.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Generally, phenotype analysis may provide unreliable identification as long as it is based on unstable expression of the markers (Drudy et al 2006;Druggan and Iversen 2009).…”

Section: Introductionmentioning

confidence: 99%

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Biochemical and molecular characterization of Cronobacter spp. (formerly Enterobacter sakazakii) isolated from foods

Turcovský

Kuniková

Drahovská

et al. 2010

Antonie van Leeuwenhoek

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“…However, the surveillance of the infections and number of incidences in different age groups are not sufficient to provide the exact number of infections attributed to this pathogen. At least 111 cases have been reported in infants and children of whom 26 were fatal 1,13,59,60,61 . Only a few of these cases are well described and most of them occurred sporadically, making epidemiological investigations impossible.…”

Section: Disease Caused By Cronobacter Sakazakiimentioning

confidence: 99%

Cronobacter sakazakii (Enterobacter sakazakii): An Emerging Foodborne Pathogen

Fakruddin¹,

Rahaman²,

Ahmed³

et al. 2013

Int J of Biomed & Adv Res

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Cronobacter sakazakii (Enterobacter sakazakii) is an emerging pathogen that causes necrotizing enterocolitis, sepsis, and meningitis. This bacterium is opportunistic pathogen and is linked with life-threatening infections in neonates and elderly persons. It is considered as ubiquitous organism and can be found in a broad range of foods & food ingredients and in water, in a variety of areas, including hospitals and houses though outbreaks most commonly associated with the ingestion of contaminated powdered infant formula (PIF). International Commission on Microbiological Specifications for Foods has ranked C. sakazakii a “severe hazard for restricted populations.” It poses high tolerance to environmental stresses such as osmotic stress, elevated temperature etc. and decontamination processes and has broad antibiotic resistance and resistance to bile salts and disinfectants. Cronobacter sakazakii may survive in macrophage cells and efficiently attach to and invade epithelial cell lines, produce exopolysaccharide, form biofilm and has active efflux pumps. Controlling the organism in the production environment, thereby reducing dissemination, necessitates the provision of suitable diagnostic tools. Appropriate measures by parents, food and infant formula manufacturers, and health care providers, as well as understanding of the pathogenesis, are important in the prevention of C. sakazakii-related infections.

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