Culture-Independent Multiplexed Detection of Drug-Resistant Bacteria Using Surface-Enhanced Raman Scattering

Abstract

The rapid and accurate detection of bacteria resistance to β-lactam antibiotics is critical to inform optimal treatment and prevent overprescription of potent antibiotics. Here, we present a fast, culture-independent method for the detection of extended-spectrum β-lactamases (ESBLs) using surface-enhanced Raman scattering (SERS). The method uses Raman probes that release sulfur-based Raman active molecules in the presence of β-lactamases. The released thiol molecules can be captured by gold nanoparticles, leading to amplified Raman signals. A broad-spectrum cephalosporin probe R1G and an ESBL-specific probe R3G are designed to enable duplex detection of bacteria expressing broad-spectrum β-lactamases or ESBLs with a detection limit of 103 cfu/mL in 1 h incubation. Combined with a portable Raman microscope, our culturing-free SERS assay has reduced screening time to 1.5 h without compromising sensitivity and specificity.

Construction and application of molecular imprinting-based surface-enhanced Raman scattering sensors

“…具有氧化特性的贵金属信 号易受其他物质的影响, 因此, 研究人员致力于开发具 有良好可回收性、高选择性和灵敏度的稳定SERS基 底 [35] . 为了解决等离子体表面没有特异性亲和力的分 析物的检测难题, 可以通过表面接枝剂对分析物进行 靶向捕获 [55] , 改进传统金属基SERS平台, 开发石墨烯 和半导体等功能材料, 进一步提高SERS灵敏度…”

A review of recent advances in the use of complex metal nanostructures for biomedical applications from diagnosis to treatment

“…By manipulating the shape of nanomaterials and the roughness of their surfaces, one can generate Raman hot spots, or areas on the nanomaterial where the optical fields are localized to enhance the electromagnetic field of the particle (Dai et al, 2023; Willets et al, 2012). As a result, effective nanomaterials for Raman imaging frequently possess edges and sharp points, such as nanostars and nanopyramids, which can have fields enhancement up to 10 15 fold due to their shapes and generation of hot spots (Smith & Gambhir, 2017).…”

Fluorescently Modified NDM-1: A Versatile Drug Sensor for Rapid In Vitro β-Lactam Antibiotic and Inhibitor Screening

“…1 To the best of our knowledge, there is currently no clinically useful NDM-1 inhibitor against NDM-1 in the past decades. 1,14 Because of their serious threat to human health, 15 there is an urgent need for searching potent drugs against NDM-type βlactamases. To this end, effective drug sensors based on NDMtype β-lactamases must be available for the discovery of new drug candidates and for antibiotic/inhibitor screening in vitro.…”