Culture-independent Characterization of the Microbiome of Healthy Pulp

Widmer, Christopher; Skutas, Jorie; Easson, Cole G.; Lopez, Jose V.; Torneck, Calvin D.; Flax, Michael; Sayın, Taner Cem

doi:10.1016/j.joen.2018.03.009

Cited by 23 publications

(28 citation statements)

References 38 publications

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“…This method can detect bacteria in the presence of 10‐10 2 cells . On the other hand, the sensitivity of culture is approximately 10 4 ‐10 5 cells for target species using non‐selective media, and 10 3 using selective media . Moreover, recent literature has shown the occurrence of bacteria in vital pulp in pristine teeth and no periodontal disease …”

Section: Discussionmentioning

confidence: 99%

“…26 On the other hand, the sensitivity of culture is approximately 10 4 -10 5 cells for target species using non-selective media, and 10 3 using selective media. 27 In conclusion, microbial composition of the RC could be modulated by the presence of periodontal disease, especially in cases of severe periodontal destruction. Moreover, RC microbiota was less complex and diverse than the one found in the PP.…”

Section: Number Of Strainsmentioning

confidence: 92%

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Microbiota of periodontal pockets and root canals in induced experimental periodontal disease in dogs

Gadê-Neto

Rodrigues

Louzada

et al. 2019

J of Invest & Clin Dent

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Aim To investigate the relationship between the microbiota of periodontal pockets (PP) and root canals (RC) in dogs submitted to experimental periodontal disease (ExPD). Methods ExPD was induced by combining cotton and wire ligatures. After 125 days, microbiological samples were collected from PP and RC. Strains isolated from 19 teeth were submitted to DNA extraction, 16S rRNA gene amplification and gene sequencing. Pearson's χ2‐ and Fisher's exact tests and McNemar's test were used when appropriate. Results The number of species in PP was greater than in RC, with prevalence of obligate anaerobes and Gram‐negative bacteria. In the PP predominated Fusobacterium necrophorum, Porphyromonas gingivalis, Prevotella loescheii, Campylobacter gracilis and Veillonella parvula. In the RC samples, 9 had microbial growth, with predominance of the following genera: Staphylococcus, Streptococcus and Neisseria. Eight genera were common to both sites in the same tooth. PP presented a greater number of species than the RC. No significant difference was observed in the species found in PP and RC in the same tooth. Conclusion Microbial composition of the RC could be modulated by the presence of periodontal disease, especially in cases of severe periodontal destruction. RC microbiota was less complex and diverse than the PP.

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Section: Discussionmentioning

confidence: 99%

Section: Number Of Strainsmentioning

confidence: 92%

Microbiota of periodontal pockets and root canals in induced experimental periodontal disease in dogs

Gadê-Neto

Rodrigues

Louzada

et al. 2019

J of Invest & Clin Dent

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“…Though commensal the gram-positive facultative anaerobic genus Staphylococcus may also become an opportunistic pathogen. Jointly Ralstonia, Acinetobacter and Staphylococcus genera contributed to less than 10% of total blood-borne microbiome (7).…”

Section: Deep Caries Microbiomementioning

confidence: 99%

“…Traditional endodontology holds the concept of sterile pulp cavity in caries free and periodontal stable teeth. However, shifting from previous culturing methods to modern microbial molecular technologies such PCR combined with HT (high-throughput) DNA sequencing, it was revealed the existence at least transiently of microorganisms even inside the pulp of presumed clinically healthy teeth (7,8). Such microorganisms, having commensal or beneficial interrelations with host, are termed microbial symbionts (7,9).…”

Section: Deep Caries Microbiomementioning

confidence: 99%

“…Additional to this core group there were also found to a lesser extent bacteria from genus Micrococcus, Corynebacterium, Burkholderia or family Enterobacteriaceae, and Micrococcaceae. This microbiome achieved the endodontic healthy system by transient bacteremia without to follow an external ingress pathway, such as caries or exposed dentinal tubules (7).…”

Section: Deep Caries Microbiomementioning

confidence: 99%

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Molecular Mechanisms of Dentine-Pulp Complex Response Induced by Microbiome of Deep Caries

Alexandru-Andrei

Perlea²,

Gheorghiu³

et al. 2019

ARS Medica Tomitana

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Deep caries progress is associated with tertiary dentin formation and additional reversible or irreversible dental pulp infl ammation. It seems that some particular signs of pain in irreversible pulpitis are associated to a particular caries microfl ora. Streptococcus species, Parvimonas micra and Dialister invisus are prevailing in cases of throbbing pain while Streptococcus mutans is incriminated in sensitivity to vertical percussion of tooth. Continuous pain is thought to be the clinical outcome of Lactobacillus implication. A better understanding of molecular signals and mechanisms induced by microbiome of deep caries that orchestrate the modulation of dental pulp complex response toward tertiary dentinogenesis or pulp infl ammation it is supposed to improve diagnosis and conservative therapies of vital pulp.

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Ancient dental pulp: Masterpiece tissue for paleomicrobiology

Anh

Drancourt

Aboudharam

2020

Molec Gen & Gen Med

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Introduction: Dental pulp with special structure has become a good reference sample in paleomicrobiology-related blood-borne diseases, many pathogens were detected by different methods based on the diagnosis of nucleic acids and proteins. Objectives: This review aims to propose the preparation process from ancient teeth collection to organic molecule extraction of dental pulp and summary, analyze the methods that have been applied to detect septicemic pathogens through ancient dental pulps during the past 20 years following the first detection of an ancient microbe. Methods: The papers used in this review with two main objectives were obtained from PubMed and Google scholar with Results: The analysis of ancient dental pulp should have a careful preparation process with many different steps to give highly accurate results, each step complies with the rules in archaeology and paleomicrobiology. After the collection of organic molecules from dental pulp, they were investigated for pathogen identification based on the analysis of DNA and protein. Actually, DNA approach takes a principal role in diagnosis while the protein approach is more and more used. A total of seven techniques was used and ten bacteria (Yersinia pestis, Bartonella quintana, Salmonella enterica serovar Typhi, Salmonella enterica serovar Paratyphi C, Mycobacterium leprae, Mycobacterium tuberculosis, Rickettsia prowazeki, Staphylococcus aureus, Borrelia recurrentis, Bartonella henselae) and one virus (Anelloviridae) were identified. Y. pestis had the most published in quantity and all methods were investigated for this pathogen, S. aureus and B. recurrentis were identified by three different methods and others only by one. The combining methods interestingly increase the positive rate with ELISA, PCR and iPCR in Yersinia pestis diagnosis. Twenty-seven ancient genomes of Y. pestis and one ancient genome of B. recurrentis were reconstructed.Comparing to the ancient bone, ancient teeth showed more advantage in septicemic diagnosis. Beside pathogen identification, ancient pulp help to distinguish species.

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Culture-independent Characterization of the Microbiome of Healthy Pulp

Cited by 23 publications

References 38 publications

Microbiota of periodontal pockets and root canals in induced experimental periodontal disease in dogs

Microbiota of periodontal pockets and root canals in induced experimental periodontal disease in dogs

Molecular Mechanisms of Dentine-Pulp Complex Response Induced by Microbiome of Deep Caries

Ancient dental pulp: Masterpiece tissue for paleomicrobiology

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