Culture-independent analysis of an endophytic core microbiome in two species of wheat: Triticum aestivum L. (cv. ‘Hondia’) and the first report of microbiota in Triticum spelta L. (cv. ‘Rokosz’)

Kuźniar, Agnieszka; Włodarczyk, Kinga; Grządziel, Jarosław; Goraj, Weronika; Gałązka, Anna; Wolińska, Agnieszka

doi:10.1016/j.syapm.2019.126025

Cited by 76 publications

(53 citation statements)

References 43 publications

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“…In Kuźniar et al [9], fungal species diversity was greater in the common wheat variety 'Hondia' than in the spelt wheat variety 'Rokosz,' while in our research the 'Zollernspellz' variety was more species-rich than the common wheat variety. The species diversity of the microbiota that inhabit the root zone may be considerable given that none of the fungal species identified in Kuźniar et al [9] were found in our study. Here, we have demonstrated that the wheat variety 'Dakotana' was the most species rich (40 isolates), while the spelt varieties contained, on average, 29 species ('Badenstern' contained 38 species).…”

Section: Discussioncontrasting

confidence: 59%

“…vulgare L. [72,73]-but only one study has focused on spelt wheat-T. aestivum ssp. spelta L. [9]. In Kuźniar et al [9], fungal species diversity was greater in the common wheat variety 'Hondia' than in the spelt wheat variety 'Rokosz,' while in our research the 'Zollernspellz' variety was more species-rich than the common wheat variety.…”

Section: Discussioncontrasting

confidence: 41%

“…spelta L. [9]. In Kuźniar et al [9], fungal species diversity was greater in the common wheat variety 'Hondia' than in the spelt wheat variety 'Rokosz,' while in our research the 'Zollernspellz' variety was more species-rich than the common wheat variety. The species diversity of the microbiota that inhabit the root zone may be considerable given that none of the fungal species identified in Kuźniar et al [9] were found in our study.…”

Section: Discussioncontrasting

confidence: 41%

“…The protection of crop plants from the effects of global climate change, which may negatively affect yields, is difficult, or even impossible, to implement. However, our knowledge of microbiota is constantly expanding due to the application of different cultivation-independent techniques based on the isolation of all 16S rRNA present in plants, which are, consequently, dedicated to the detection of both cultivable and non-cultivable endophytes [9]. New techniques have significantly improved the understanding of the role of the plant microbiome and have proven that a plant can adapt to a changing environment by involving different components of its microbiome through the selection of at least part of their ecto-and endospheric microbiota to better adapt to environmental constraints [10].…”

Section: Introductionmentioning

confidence: 99%

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Growth and Photosynthetic Activity of Selected Spelt Varieties (Triticum aestivum ssp. spelta L.) Cultivated under Drought Conditions with Different Endophytic Core Microbiomes

Ratajczak

Sulewska

Błaszczyk

et al. 2020

IJMS

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The role of the microbiome in the root zone is critically important for plants. However, the mechanism by which plants can adapt to environmental constraints, especially water deficit, has not been fully investigated to date, while the endophytic core microbiome of the roots of spelt (Triticum aestivum ssp. spelta L.) grown under drought conditions has received little attention. In this study, we hypothesize that differences in the endophytic core of spelt and common wheat root microbiomes can explain the variations in the growth and photosynthetic activity of those plants, especially under drought conditions. Our greenhouse experimental design was completely randomized in a 2 × 4 × 3 factorial scheme: two water regime levels (well-watered and drought), three spelt varieties (T. aestivum ssp. spelta L.: ‘Badenstern’, ‘Badenkrone’ and ‘Zollernspelz’ and one wheat variety: T. aestivum ssp. vulgare L: ‘Dakotana’) and three mycorrhizal levels (autoclaved soil inoculation with Rhizophagus irregularis, control (autoclaved soil) and natural inoculation (non-autoclaved soil—microorganisms from the field). During the imposed stress period, relative water content (RWC), leaf chlorophyll fluorescence, gas exchange and water use efficiency (WUE) were measured. Microscopic observations of the root surface through fungi isolation and identification were conducted. Our results indicate that ‘Badenstern’ was the most drought tolerant variety, followed by ‘Zollernspelz’ and ‘Badenkrone,’ while the common wheat variety ‘Dakotana’ was the most drought sensitive. Inoculation of ‘Badenstern’ with the mycorrhizal fungi R. irregularis contributed to better growth performance as evidenced by increased whole plant and stalk dry matter accumulation, as well as greater root length and volume. Inoculation of ‘Zollernspelz’ with arbuscular mycorrhizal fungi (AMF) enhanced the photochemical efficiency of Photosystem II and significantly improved root growth under drought conditions, which was confirmed by enhanced aboveground biomass, root dry weight and length. This study provides evidence that AMF have the potential to be beneficial for plant growth and dry matter accumulation in spelt varieties grown under drought conditions.

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Section: Discussioncontrasting

confidence: 59%

Section: Discussioncontrasting

confidence: 41%

Section: Discussioncontrasting

confidence: 41%

Section: Introductionmentioning

confidence: 99%

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Growth and Photosynthetic Activity of Selected Spelt Varieties (Triticum aestivum ssp. spelta L.) Cultivated under Drought Conditions with Different Endophytic Core Microbiomes

Ratajczak

Sulewska

Błaszczyk

et al. 2020

IJMS

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“…Pseudomonas is a genus that consists of common bacterial endophytes that colonize different crops species. In a study with wheat cultivars, it even was the only endophytic genus colonizing two cultivars throughout a number of different plant stages -from the endosperm stage to the leaf formation stage (Kuźniar et al 2020). Several strains of Pseudomonas are known to cause induced systemic resistance in plants which protects the plant from insect herbivores, and induce growth promotion in both shoots and roots (e.g.…”

Section: Discussionmentioning

confidence: 99%

Soil inoculation alters the endosphere microbiome of chrysanthemum roots and leaves

et al. 2020

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Aims This study examines how inoculation with live soil influences the assembly of the endosphere microbiome of leaves and roots of chrysanthemum. Methods Sterilized soil was inoculated with 10% soil in which grasses had grown. Chrysanthemum was planted in these soils and control plants were grown in 100% sterilized soil. All plants were exposed to thrips, and leaves and roots were collected from inoculated and control plants that experienced high and low thrips damage. DNA was extracted and the bacterial and fungal community inside roots and leaves was determined using Illumina sequencing. Results Inoculation increased bacterial diversity in root but not in leaf tissues. The endosphere of both roots and leaves was dominated by Pseudomonadaceae. In leaves, the relative abundance of Pseudomonadaceae was higher in inoculated than in control plants, whereas this was opposite in roots. Leaves contained more rare bacterial families than roots. The number of fungal reads was very low and the endopshere did not differ between plants with high or low thrips damage. Conclusions Bacterial communities inside chrysanthemum root and leaf tissues differ considerably. Soil inoculation with entire microbiomes can be used to change root and foliar bacterial microbiomes and this is particularly effective in root tissues.

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Cultromic and Metabarcodic Insights into Saffron-Microbiome Associations

Bhagat

Mansotra

Ambardar

et al. 2022

Compendium of Plant Genomes

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Culture-independent analysis of an endophytic core microbiome in two species of wheat: Triticum aestivum L. (cv. ‘Hondia’) and the first report of microbiota in Triticum spelta L. (cv. ‘Rokosz’)

Cited by 76 publications

References 43 publications

Growth and Photosynthetic Activity of Selected Spelt Varieties (Triticum aestivum ssp. spelta L.) Cultivated under Drought Conditions with Different Endophytic Core Microbiomes

Growth and Photosynthetic Activity of Selected Spelt Varieties (Triticum aestivum ssp. spelta L.) Cultivated under Drought Conditions with Different Endophytic Core Microbiomes

Soil inoculation alters the endosphere microbiome of chrysanthemum roots and leaves

Cultromic and Metabarcodic Insights into Saffron-Microbiome Associations

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