Culture factors in relation to the success of human in vitro fertilization and embryo transfer

Quinn, Patrick; Warnes, Graham M.; Kerin, John F.; Kirby, Christine A.

doi:10.1016/s0015-0282(16)47591-6

Cited by 125 publications

(38 citation statements)

References 14 publications

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“…Thus, researchers may consider practicing the sterile technique strictly and selecting culture instruments carefully. Washing the instruments with the culture medium immediately before use is recommended in some special cases 151, 152, 153…”

Section: Current Issues With Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

374

278

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BackgroundCell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media.MethodsA literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords.ResultsAt the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances.ConclusionsFurther improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.

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Section: Current Issues With Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

374

278

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“…The oviducts were divided into three parts representing the ampullary segment, the ampullary-isthmic junction and the isthmic segment. The three segments of both oviducts were flushed with 5 ml Hepes-buffered Whittingham's T6 culture medium adjusted to pH 7-4 (Quinn et al, 1984). The flushings were checked for the presence of ova and embryos under a stereomicroscope (Wild M8).…”

Section: Methodsmentioning

confidence: 99%

Effect of endometriosis on ovulation, ovum pickup, fertilization and tubal transport in the rabbit

Dunselman

Land²,

Bouckaert³

et al. 1988

Reproduction

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Summary. In 25 rabbits (Group E) endometrium from the right uterine horn was transplanted onto the peritoneum. In 25 rabbits (Group C) fat was transplanted. After a recovery period of 12 weeks the does were mated, and killed 24 h later. In Group E the implants had changed into cysts of 5\p=n-\15mm in diameter. Histological examination revealed endometrial glands and stroma in every specimen. Periadenexal adhesions did not develop in any animal. No differences were found between Groups C and E in the number of corpora lutea, the recovery rate, the fertilization rate and the transport of fertilized ova. These findings indicate that endometrial implants in the rabbit have no influence on the ovulatory mechanism, the pickup function of the oviduct, the fertilization rate or on the transport of fertilized ova. Taking into account the restrictions of a rabbit model, it is suggested that the decreased fecundity in mild endometriosis in the human may be caused by disturbances in post-fertilization events, i.e. development of the pre-implantation embryo or implantation.

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“…Embryos were cultured in drops of M16 medium (Fulton and Whittingham, 1978) or modified M16, referred to as T6 medium (Quinn et al, 1984), under paraffin oil (Merck, and Corning), in (Falcon or Sterilin) Petri dishes, at 37°C (40°C), in the atmosphere of 5% CO 2 in the air. Paraffin oil was equilibrated with the medium in the above mentioned conditions for 24 h before starting the culture.…”

Section: Isolation Of Embryos and Culture In Vitromentioning

confidence: 99%

Trophoblastic vesicles as carriers of embryonic cells for mammalian cloning

Modliński¹,

Stefanski²,

Gorniewska³

et al. 2004

J. Anim. Feed Sci.

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Inner cell mass-free blastocysts, also referred to as trophoblastic vesicles (TVs) could be used as carriers to be injected with in vitro cultured embryonic cells and to generate clones of animals, providing an alternative method of mammalian cloning. Here we compare three methods of obtaining mouse TVs. Culturing preimplantation embryos in heat shock conditions brings about up to 35% of TVs having up to 47 cells and the efficiency is dependent on the stage of cultured embryos. Live birth was obtained after transfer of ES cell-injected TVs to recipient mice. Culture with radioactive precursor yields up to 55% of TVs, but only at concentrations supplying 37% TVs is their cell number sufficiently high. The effects of PMA treatment are 47% of TVs.

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Culture factors in relation to the success of human in vitro fertilization and embryo transfer

Cited by 125 publications

References 14 publications

Animal‐cell culture media: History, characteristics, and current issues

Animal‐cell culture media: History, characteristics, and current issues

Effect of endometriosis on ovulation, ovum pickup, fertilization and tubal transport in the rabbit

Trophoblastic vesicles as carriers of embryonic cells for mammalian cloning

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