Culture expansion of primary human nasal epithelial cells (NEC) isolated with a nasal scraping spoon
Li Hangjin,
Yin Junting,
Wang Yiqin
et al.
Abstract:Objective To obtain high-purity nasal epithelial cells (NEC) while avoiding the irritation experienced by patients during nasal biopsies. Methods This prospective, observational study enrolled patients undergoing surgical treatment for nasal septum deviation. After general anaesthesia, a novel nasal scraping spoon was used to collect epithelial cells from the mid-part of the inferior turbinate. The cells were evenly plated on six-well plates coated with rat tail collagen. The morphology and growth of the cells… Show more
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